Spermidine and abscisic acid-mediated phosphorylation of a cytoplasmic protein from rice root in response to salinity stress

• Autorzy: Gupta K. , Gupta B. , Ghosh B. , Sengupta D. N.
• Języki publikacji: EN

Abstrakty

EN

Importance of higher polyamines, spermidine, and spermine, in relation to the mechanism and adaptation to combat abiotic stress has been well established in cereals. Owing to their polycationic nature at physiological pH, polyamines bind strongly to negative charges in cellular components such as nucleic acids, various proteins, and phospholipids. To study the physiological role of polyamine during salinity stress, phosphorylation study was carried out in cytosolic soluble protein fraction isolated from the roots of salt tolerant (Nonabokra) and salt sensitive (M-1-48) rice cultivars treated with none (control), NaCl (150 mM, 16 h), spermidine (1 mM, 16 h) or with abscisic acid (100 µM, 16 h).A calcium independen auto regulatory 42 kDa protein kinase was found to phosphorylate myelin basic protein and casein but not histone. Interestingly, this was the only protein to be phosphorylated in root cytosolic fraction during NaCl/ abscisic acid/spermidine treatment indicating its importance in salinity mediated signal transduction. This is the first report of polyamine as well as abscisic acid induced protein kinase activity in rice root in response to salinity stress.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 1
• Opis fizyczny: p.29-40,fig.,ref.

Twórcy

autor

Gupta K.

• Plant Molecular Biology Division, Bose Institute, 93/1 A.P.C. Road, Kolkata 700009, India
• Plant Molecular Biology, Department of Botany, Bethune College, 181-Bidhan Sarani, Kolkata 700006, India

autor

Gupta B.

• Plant Molecular Biology Division, Bose Institute, 93/1 A.P.C. Road, Kolkata 700009, India
• Molecular Biology Laboratory, Department of Biotechnology, Presidency University, 86/1 College Street, Kolkata 700073, India

autor

Ghosh B.

• Plant Molecular Biology Division, Bose Institute, 93/1 A.P.C. Road, Kolkata 700009, India

autor

Sengupta D. N.

• Plant Molecular Biology Division, Bose Institute, 93/1 A.P.C. Road, Kolkata 700009, India

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