Comparative analysis of essential oil composition and oil containing glands in Ocimum sanctum L. (Holy basil) under ambient and supplemental level of UV-B through gas chromatography–mass spectrometry and scanning electron microscopy

• Autorzy: Kumari R. , Agrawal S. B.
• Języki publikacji: EN

Abstrakty

EN

Field grown plants of holy basil (Ocimum sanctum L.) were treated with biologically effective supplemental ultraviolet-B (sUV-B) radiation (ambient +1.8 kJ m⁻² day⁻¹) to evaluate its effect on specialized oil glands and quantity and quality of the essential oil. The total yield of essential oil increased significantly by 42.0% after sUV-B treatment. Gas chromatography–mass spectrometry analysis showed that most important flavor compounds β-caryophyllene, germacrene-D, ethyl linoleolate, β-elemene and camphenol increased significantly after sUV-B treatment. Moreover, the level of main compound eugenol was not affected significantly. Scanning electron microscopy analysis showed turgidity of oil glands after sUV-B exposure and it was well correlated with the higher yield of essential oil. The result is of specific interest because if other parameters remain constant, sUV-B may affect the production of the essential oil in Ocimum sanctum L.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 4
• Opis fizyczny: p.1093-1101,fig.,ref.

Twórcy

autor

Kumari R.

• Department of Botany, Banaras Hindu University, Varanasi 221005, India

autor

Agrawal S. B.

• Department of Botany, Banaras Hindu University, Varanasi 221005, India

Bibliografia

• Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectrometry. Allured Publishing Corporation, Carol Stream
• Allman MA, Pena MM, Pang D (1995) Supplementation with flaxseed oil versus sunflower seed oil in healthy young men consuming a low fat diet: effects on platelet composition and function. Eur J Clin Nutr 49:169–178
• Asha MK, Prashanth D, Murli B, Padmaja R, Amit A (2001) Anthelmintic activity of essential oil of Ocimum sanctum and eugenol. Fitoterapia 72:669–670
• Caldwell MM (1971) Solar ultraviolet radiation and the growth and development of higher plants. In: Giese AC (ed) Phytophysiology, vol 6. Academic Press, New York, pp 131–177
• Chang X, Alderson PG, Wright CJ (2009) Enhanced UV-B radiation alters basil (Ocimum basilicum L.) growth and stimulates the synthesis of volatile oils. J Hortic For 1:027–031
• Clevenger JH (1928) Apparatus for the determination of volatile oil. J Am Pharm Assoc 17:346
• Dasgupta R, Bhaumik DK (1995) Upper and lower tolerance limits of atmospheric ozone level and extreme value distribution. Indian J Stat 57(series B):182–199
• Gang DR, Wang JH, Dudareva N, Nam KH, Simon JE, Lewinsohn E, Pichersky E (2001) An investigation of the storage and bioynthesiss of phenylpropenes in basil. Plant Physiol 125:539–555
• Green AES, Cross KR, Smith LA (1980) Improved analytic characterization of ultraviolet skylight. Photochem Photobiol 31:59–65
• Guenette SA, Rodd A, Marier JF, Beaudry F, Vachon P (2007) Pharmacokinetics of eugenol and its effects on thermal hypersensitivity in rats. Eur J Pharmacol 562:60–67
• Hannan MA, Marenah L, Ali L, Rokeya B, Flatt PR, Abdel-Wahab YHA (2006) Ocimum sanctum leaf extracts stimulate insulin secretion from perfused pancreas, isolated islets and clonal pancreatic β-cells. J Endocrinol 189:127–136
• He M, Du M, Fan M, Bian Z (2007) In vitro activity of eugenol against Candida albicans biofilms. Mycopathologia 163:137–143
• Hidema J, Kumagai T (2006) Sensitivity of rice to ultraviolet-B radiation. Ann Bot 97:933–942
• Hussain AI, Anwar F, Sherazi STH, Przybylski R (2008) Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chem 108:986–995
• Ioannidis D, Bonner L, Johnson CB (2002) UV-B is required for normal development of oil glands in Ocimum basilicum L. (sweet basil). Ann Bot 90:453–460
• Johnson CB, Kirby J, Naxakis G, Pearson S (1999) Substantial UV-Bmediated induction of essential oils in sweet basil (Ocimum basilicum L.). Phytochemistry 51:507–510
• Juteau F, Masotti V, Bessiere JM, Dherbomez M, Viano J (2002) Antibacterial and antioxidant activities of Artemisia annua essential oil. Fitoterapia 73:532–535
• Kakani VG, Reddy KR, Zhao D, Shailja K (2003) Field crop responses to ultraviolet-B radiation: a review. Agric For Meteor 120:191–218
• Karousou R, Grammatikopoulos G, Lanaras T, Manetas Y, Kokkini S (1998) Effect of enhanced UV-B radiation on Mentha spicata essential oil. Phytochemistry 49:2273–2277
• Kothari SK, Bhattacharya AK, Ramesh S (2004) Essential oil yield and quality of methyl eugenol rich Ocimum tenuiflorum L., (syn. O. sanctum L.) grown in south India as influenced by method of harvest. J Chromatography A 1054:67–72
• Kumar A, Shukla R, Singh P, Dubey NK (2009) Chemical composition, antifungal and antiaflatoxigenic activities of Ocimum sanctum L. essential oil and its safety assessment as plant based antimicrobial. Food Chem Toxicol. doi:10/1016/j.fct. 2009.11.028
• Kumari R, Agrawal SB, Sarkar A (2009a) Evaluation of changes in oil cells and composition of essential oil in Lemongrass (Cymbopogon citratus (D.C.) Stapf.) due to supplemental ultraviolet-B irradiation. Curr sci 97:1137–1142
• Kumari R, Agrawal SB, Singh S, Dubey NK (2009b) Supplemental ultraviolet-B induced changes in essential oil composition and total phenolics of Acorus calamus L., (Sweet flag). Ecotoxicol Environ safety 72:2013–2019
• Maffei M, Scannerini S (2000) UV-B effect on photomorphogenesis and essential oil composition in peppermint (Mentha piperita L.). J Essent Oil Res 12:523–529
• McKenzie RL, Aucamp PJ, Bais AF, Bjorn LO, Ilyase M (2007) Changes in biologically active ultraviolet radiation reaching the earth’s surface. Photochem Photobiol Sci 6:218–231
• Medeiros R, Passos GF, Vitor CE, Koepp J, Mazzuco TL, Pianowski LF, Campos MM, Calixtol JB (2007) Effect of two active compounds obtained from the essential oil of Cordia verbenacea on the acute inflammatory responses elicited by LPS in the rat paw. British J Pharmacol 151:618–627
• Mondal S, Mahapatra SC, Mirdha BR, Naik SN (2007) Antimicrobial activities of essential oils obtained from fresh and dried leaves of Ocimum sanctum (L.) against enteric bacteria and yeast. In: ISHS Acta Horticult 756 International Symposium on Medicinal and Nutraceutical Plants
• Mukherji SP (1987) Ocimum- cheap source of Eugenol. Science Reporter P-599
• Muthuraman A, Diwan V, Jaggi AS, Singh N, Singh D (2008) Ameliorative effects of Ocimum sanctum in sciatic nerve transection-induced neuropathy in rats. J Ethnopharmacol 120:56–62
• Nitz GM, Schnitzler WH (2004) Effect of PAR and UV-B radiation on the quality and quantity of the essential oil in sweet basil (Ocimum basilicum L.). Acta Horticult (ISHS) 659:375–381
• O’Brien TP, McCully ME (1981) The study of plant structure, principles and selected methods. Thermacarphi Pty Ltd, Melbourne, Australia
• Prakash P, Gupta N (2005) Therapeutic uses of Ocimum sanctum Linn (tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian J Physiol Pharmacol 49:125–131
• Rathore D, Agrawal SB, Singh A (2003) Influence of supplemental UV-B radiation and mineral nutrients on biomass, pigments and yield of two cultivars of wheat. Int J Biotron 32:1–15
• Sahoo A, Sarkar S, Singh RP, Kafatos M, Summers ME (2005) Declining trend of total ozone column over the northern parts of India. Int J Remote Sens 26:3433–3440
• Simic A, Rancic A, Sokovic MD, Ristic M, Grujic-Jovanovic S, Vukojevic J, Marin PD (2008) Essential oil composition of Cymbopogon winterianus and Carum carvi and their antimicrobial activities. Pharmaceut Biol 46:437–441
• Tiku AB, Abraham SK, Kale RK (2004) Eugenol as an in vivo radioprotective agent. J Rad Res 45:435–440
• Tundis R, Loizzo MR, Bonesi M, Menichini F, Dodaro D, Passalacqua NG, Statti G, Menichini F (2009) In vitro cytotoxic effects of Senecio stabianus Lacaita (Asteraceae) on human cancer cell lines. Nat Prod Res 23:1707–1718
• Vani SR, Cheng SF, Chuah CH (2009) Comparative Study of Volatile Compounds from Genus Ocimum. Am J Applied Sci 6:523–528
• Wei Y, Zhang X, Wei L, Yang XS (2005) Analysis of the constituents of volatile oil from Pimpinella candolleana Wight et Arn. J Guiyang Trad Chinese Med Univ 27:56–57
• Zheljazkov VD, Cantrell CL, Evans WB, Ebelhar MW, Coker C (2008) Yield and composition of ocimum basilicum L. and ocimum sanctum L. grown at four locations. Hortic Sci 43:737–774

Uwagi

PL

Rekord w opracowaniu