Effects of storage in different semen extenders on the pre - freezing and post - thawing quality of boar spermatozoa

• Autorzy: Dziekonska A. , Zasiadczyk L. , Lecewicz M. , Strzezek R. , Koziorowska-Gilun M. , Fraser R. , Mogielnicka-Brzozowska M. , Kordan W.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to investigate the effects of storage of semen in different commercial extenders on the pre-freezing and post-thawing quality of boar spermatozoa. Semen was diluted in BTS, Androhep (AH) and Gedil (GD), stored for 24 h at 17°C, and then frozen in accordance with the cryopreservation protocol. Analyses of the quality of spermatozoa included: motility, normal apical ridge (NAR) acrosome, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), measurements of ATP content and activity of superoxidase dismutase (SOD) and glutathione peroxidase (GPx). Prior to the freezing process, no significant effect of the extender on the sperm quality parameters was noted. After thawing the spermatozoa it was demonstrated that the type of extender used influenced PMI, MMP, ATP content and activity of GPx. In the AH extender the percentage of spermatozoa with PMI and ATP content in spermatozoa was significantly higher (P<0.05) as compared to the BTS or GD extenders. In addition, semen stored in the AH was characterised by a statistically higher (P<0.05) percentage of spermatozoa with MMP and increased activity of GPx as compared with the BTS. The results obtained indicate that for the cryopreservation process, boar spermatozoa stored for 24 hours in liquid state can be used. However, the type of extender used prior to freezing may have a significant effect on the post- thawing quality of the spermatozoa. The AH extender better secured the quality of thawed boar spermatozoa as compared with the BTS or GD.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 4
• Opis fizyczny: p.733-740,ref.

Twórcy

autor

Dziekonska A.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

autor

Zasiadczyk L.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

autor

Lecewicz M.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

autor

Strzezek R.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

autor

Koziorowska-Gilun M.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

autor

Fraser R.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

autor

Mogielnicka-Brzozowska M.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

autor

Kordan W.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

Bibliografia

• Agarwal A, Makker K, Sharma R (2008) Clinical relevance of oxidative stress in male factor infertility: an update. Am J Reprod Immunol 59: 2-11.
• Alvarez JG, Storey BT (1992) Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation. J Androl 13: 232-241.
• Alvarez JG, Storey BT (1995) Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 42: 334-346.
• Bailey JL, Lessard C, Jacques J, Bréque C, Dobrinski I, Zeng W, Galantino-Homer HL (2008) Cryopreservation of boar semen and its future importance to the industry. Theriogenology 70: 1251-1259.
• Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C (2000) Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol Reprod Dev 55: 282-288.
• Blank M, Soo L, Britten JS (1976) Adsorption of albumin on rabbit sperm membranes. J Membr Biol 29: 401-409.
• Cerolini S, Maldjian A, Surai P, Noble R (2000) Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Anim Reprod Sci 58: 99-111.
• Didion BA, Braun GD, Duggan MV (2013) Field fertility of frozen boar semen: a retrospective report comprising over 2600 AI services spanning a four year period. Anim Reprod Sci 137: 189-196.
• Dubé C, Beaulieu M, Reyes-Moreno C, Guillemette C, Bailey JL (2004) Boar sperm storage capacity of BTS and Androhep Plus: viability, motility, capacitation, and tyrosine phosphorylation. Theriogenology 62: 874-886.
• Dziekońska A, Fraser L, Strzezek J (2009) Effect of different storage temperatures on the metabolic activity of spermatozoa following liquid storage of boar semen. J Anim Feed Sci 18: 638-649.
• Dziekońska A, Fraser L, Majewska A, Lecewicz M, Zasiadczyk Ł, KordanW (2013) Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17 degrees C. Pol J Vet Sci 16: 517-525.
• El-Kon I (2011) Testing Usability of Bovine Serum Albumin (BSA) for Preservation of Egyptian Buffalo Semen. American-Eurasian J Agric & Environ Sci 11: 495-502.
• Fraser L, Dziekońska A, Strzeżek R, Strzeżek J (2007) Dialysis of boar semen prior to freezing-thawing: its effects on post-thaw sperm characteristics. Theriogenology 67: 994-1003.
• Fraser L, Strzeżek J (2007) Effect of different procedures of ejaculate collection, extenders and packages on DNA integrity of boar spermatozoa following freezing-thawing. Anim Reprod Sci 99: 317-329.
• Funahashi H, Sano T (2005) Select antioxidants improve the function of extended boar semen stored at 10 degrees C. Theriogenology 63: 1605-1616.
• Gadea J (2003) Review: Semen extenders used in the artificial insemination of swine. Spanish J Agric Res 1: 17-27.
• Garner DL, Johnson LA (1995) Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod 53: 276-284.
• Gączarzewicz D, Piasecka M, Udała J, Błaszczyk B, Stankiewicz T, Laszczyńska M (2010) Plasma membrane changes during the liquid storage of boar spermatozoa: a comparison of methods. Acta Vet Hung 58: 105-116.
• Gączarzewicz D, Udała J, Piasecka M, Błaszczyk B, Stankiewicz T (2015) Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability. Tur J Biol 39: 582-594.
• Gerrits RJ, Lunney JK, Johnson LA, Pursel VG, Kraeling RR, Rohrer GA, Dobrinsky JR (2005) Perspectives for artificial insemination and genomics to improve global swine populations. Theriogenology 63: 283-299.
• Guthrie HD, Welch GR (2006) Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry. J Anim Sci 84: 2089-2100.
• Guthrie HD, Welch GR (2012) Effects of reactive oxygen species on sperm function. Theriogenology 78: 1700-1708.
• Harrison RA, Dott HM, Foster GC (1982) Bovine serum albumin, sperm motility, and the „dilution effect.” J Exp Zool 222: 81-88.
• Jelezarsky L, Vaisberg C, Chaushev T, Sapundjiev E (2008) Localization and characterization of glutathione peroxidase (GPx) in boar accessory sex glands, seminal plasma, and spermatozoa and activity of GPx in boar semen. Theriogenology 69: 139-145.
• Johnson LA, Weitze KF, Fiser P, Maxwell WM (2000) Storage of boar semen. Anim Reprod Sci 62: 143-172.
• Klimont M, Wrona Z, Brodzki P, Krakowski L (2015) Influence of season and storage of boar semen on the degree of DNA fragmentation of sperm. Med Weter 71: 171-175.
• Kowalowka M, Wysocki P, Fraser L, Strzeżek J (2008) Extracellular superoxide dismutase of boar seminal plasma. Reprod Domest Anim 43: 490-496.
• Koziorowska-Gilun M, Koziorowski M, Fraser L, Strzeżek J (2011) Antioxidant defence system of boar cauda epididymidal spermatozoa and reproductive tract fluids. Reprod Domest Anim 46: 527-533.
• Peńa FJ, Garcta BM, Samper JC, Aparicio IM, Tapia JA, Ferrusola CO (2011) Dissecting the molecular damage to stallion spermatozoa: the way to improve current cryopreservation protocols? Theriogenology 76: 1177-1186.
• Roca J, Hernández M, Carvajal G, Vázquez JM, Martinez EA (2006) Factors influencing boar sperm cryosurvival. J Anim Sci 84: 2692-2699.
• Sariözkan S, Türk G, Cantürk F, Yay A, Eken A, Akçay A (2013) The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology 67: 1-6.
• Strzeżek J (2002) Secretory activity of boar seminal vesicle glands. Reprod Biol 2: 243-266.
• Strzeżek J, Glogowski J, Hopfer E, Wojtkiewicz K (1985) The „Kortowska” method of boar’s semen freezing. Med Weter 41: 349-353.
• Thomas CA, Garner DL, DeJarnette JM, Marshall CE (1998) Effect of cryopreservation of bovine sperm organelle function and viability as determined by flow cytometry. Biol Reprod 58: 786-793.
• Watson PF (1975) Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet Rec 97: 12-15.
• Wrona Z, Klimont M, Krakowski L (2009) Attempt at estimating the correlation between selected parameters of boar spermatozoa movement indicators and the time of spermatozoa storage in standard conditions. Med Weter 65: 194-197.
• Zhang XG, Yan GJ, Hong JY, Su ZZ, Yang GS, Li QW, Hu JH (2015) Effects of bovine serum albumin on boar sperm quality during liquid storage at 17°C. Reprod Domest Anim 50: 263-269.

Identyfikatory

DOI

10.1515/pjvs-2015-0095