Antimicrobial and antiviral activity-guided fractionation from Scutia buxifolia Reissek extracts

• Autorzy: Boligon A.A. , Kubica T. F. , Mario D. N. , de Brum T. F. , Piana M. , Weiblen R. , Lovato L. , Alvez S. H. , Santos R. C. V. , dos Santos Alvez C. F. , Athayde M. L.
• Języki publikacji: EN

Abstrakty

EN

Antimicrobial and antiviral activities of the fractions from Scutia buxifolia stem bark and leaves were evaluated. Best antimicrobial results occurred with the ethyl acetate (EA) and n-butanolic (NB) fractions from the leaves against Micrococcus sp. (minimal inhibitory concentration— MIC = 62.5 μg/ml), and NB fraction from stem bark and leaves against Klebsiella pneumoniae and Enterococcus faecalis (MIC = 62.5 μg/ml). The most active fractions were selected and fractioned into silica column to perform an in vitro antibiofilm assay, which evidenced subfractions EA2 and EA3 as the more active against Candida albicans (biofilm inhibitory concentration— BIC = 582 ± 0.01 lg/ml) and Staphylococcus aureus (BIC = 360 ± 0.007 lg/ml), respectively. The NB (selectivity index—SI = 25.78) and the EA (SI = 15.97) fractions from the stem bark, and the EA (SI = 14.13) fraction from the leaves exhibited a potential antiviral activity towards Herpes Simplex Virus type 1 whereas EA2 and EA3 subfractions from leaves (SI = 12.59 and 10.06, respectively), and NB2 subfraction from stem bark (SI = 12.34) maintained this good activity. Phenolic acids and flavonoids (gallic acid, chlorogenic acid, caffeic acid, rutin, isoquercitrin, quercitrin and quercetin) were identified by HPLC and may be partially responsible for the antimicrobial and antiherpes activities observed. The results obtained in this study showed that Scutia buxifolia has antibiofilm and anti-herpetic activities and that these properties are reported for the first time for this species.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 07
• Opis fizyczny: p.2229-2239,fig.,ref.

Twórcy

autor

Boligon A.A.

• Laborato´rio de Fitoquı´mica, Departamento de Farma´cia Industrial, UFSM, Universidade Federal de Santa Maria, Campus Camobi, pre´dio 26, sala 1115, Santa Maria, RS 97105-900, Brazil

autor

Kubica T. F.

• Laborato´rio de Virologia, Departamento de Medicina Veterina´ria Preventiva, UFSM, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil

autor

Mario D. N.

• Laborato´rio de Pesquisas Micolo´gicas, Departamento de Microbiologia e Parasitologia da UFSM, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazi

autor

de Brum T. F.

• Laborato´rio de Fitoquı´mica, Departamento de Farma´cia Industrial, UFSM, Universidade Federal de Santa Maria, Campus Camobi, pre´dio 26, sala 1115, Santa Maria, RS 97105-900, Brazil

autor

Piana M.

• Laborato´rio de Fitoquı´mica, Departamento de Farma´cia Industrial, UFSM, Universidade Federal de Santa Maria, Campus Camobi, pre´dio 26, sala 1115, Santa Maria, RS 97105-900, Brazil

autor

Weiblen R.

• Laborato´rio de Virologia, Departamento de Medicina Veterina´ria Preventiva, UFSM, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil

autor

Lovato L.

• Laborato´rio de Virologia, Departamento de Medicina Veterina´ria Preventiva, UFSM, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil

autor

Alvez S. H.

• Laborato´rio de Pesquisas Micolo´gicas, Departamento de Microbiologia e Parasitologia da UFSM, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazi

autor

Santos R. C. V.

• Laborato´rio de Microbiologia Clı´nica, Cieˆncias da Sau´de, UNIFRA, Centro Universita´rio Franciscano, Santa Maria, RS 97010-032, Brazil

autor

dos Santos Alvez C. F.

• Laborato´rio de Microbiologia Clı´nica, Cieˆncias da Sau´de, UNIFRA, Centro Universita´rio Franciscano, Santa Maria, RS 97010-032, Brazil

autor

Athayde M. L.

• Laborato´rio de Fitoquı´mica, Departamento de Farma´cia Industrial, UFSM, Universidade Federal de Santa Maria, Campus Camobi, pre´dio 26, sala 1115, Santa Maria, RS 97105-900, Brazil

Bibliografia

• Artani N, Marifa Y, Hanafi M (2006) Isolation and identification of active antioxidant compound from Star fruit (Averrhoa carambola) Mistletoe (Dendrophthoe pentandra L.) Miq. ethanol extract. J Appl Sci 68:1659–1663
• Boligon AA, Pereira RP, Feltrin AC, Machado MM, Janovik V, Rocha JBT, Athayde ML (2009) Antioxidant activities of flavonol derivatives from the leaves and stem bark of Scutia buxifolia Reiss. Bioresour Technol 100:6592–6598
• Boligon AA, Janovik V, Feltrin AC, Machado MM, Frohlich JK, Athayde ML (2010) Phytoconstituents isolated from dichloromethane fraction of Scutia buxifolia Reissek stem bark. Lat Am J Pharm 29(3):450–453
• Boligon AA, Brum TF, Frohlich JK, Froeder ALF, Athayde ML (2012a) HPLC/DAD profile and determination of total phenolics, flavonoids, tannins and alkaloids contents of Scutia buxifolia Reissek stem bark. Res J Phytochem 6(3):84–91
• Boligon AA, Janovik V, Frohlich JK, Spader TB, Froeder ALF, Alves SH, Athayde ML (2012b) Antimicrobial and cytotoxic activities of leaves, twigs and stem bark of Scutia buxifolia Reissek. Nat Prod Res 26:939–944
• Boligon AA, Agertt V, Janovik V, Cruz RC, Campos MMA, Guillaume D, Athayde ML, dos Santos ARS (2012c) Antimycobacterial activity of the fractions and compounds from Scutia buxifolia. Brazilian J Pharmacog 22:45–52
• Bourne KZ, Bourne N, Reising SF, Stanberry LR (1999) Plant products as topical microbicide candidates: assessment of in vitro and in vivo activity against herpes simplex virus type 2. Antivir Res 42:219–226
• Chiang LC, Chiang W, Chang MY, Ng LT, Lin CC (2002) Antiviral activity of Plantago major extracts and related compounds in vitro. Antivi Res 55(1):53–62
• Chiang LC, Chiang W, Liu MC, Lin CC (2003) In vitro antiviral activities of Caesalpinia pulcherrima and its related flavonoids. J Antimicrob Chemother 52:194–198
• Cowan MM (1999) Plant products as antimicrobial agents. Clin Microbiol Rev 12:564–582
• Duarte MCT, Leme EE, Delarmelina C, Soares AA, Figuera GM, Sartoratto A (2007) Activity of essential oils from Brazilian medicinal plants on Escherichia coli. J Ethnopharmacol 111:197–201
• Ellof JN (2004) Quantifying the bioactivity of plant extracts during screening and bioassay guided fractionation. Phytomedicine 11:370–371
• Esquenazi D, Wigg MD, Miranda MMFS, Rodrigues HM, Tostes JBF, Rozental S, Silva AJR, Alviano CS (2002) Antimicrobial and antiviral activeities of polyphenolics from Cocos nucifera Linn. (Palmae) husk fiber extract. Res Microbiol 153:647–652
• Fontenelle ROS, Morais SM, Brito EH, Brihante RS, Cordeiro RA, Nascimento NR, Kerntopf MR, Sidrim JJ, Rocha MF (2008) Antifungal activity of essential oils of Croton species from the Brazilian Caatinga biome. J Appl Microbiol 104:1383–1390
• Freitas AM, Almeida MTR, Andrighetti-Fro¨hner CR, Cardozo FTGS, Barardi CRM, Farias MR, Simo˜es CMO (2009) Antiviral activity-guided fractionation from Araucaria angustifolia leaves extract. J Ethnopharmacol 126:512–517
• Gescher K, Ku¨hn J, Hafezi W, Louis A, Derksen A, Deters A, Lorentzen E, Hensel A (2011) Inhibition of viral adsorption and penetration by an aqueous extract from Rhododendron ferrugineum L. as antiviral principle against herpes simplex virus type-1. Fitoterapia 82(3):408–413
• Gilbert C, Bestman-Smith J, Boivin G (2002) Resistance of herpesviruses to antiviral drugs; Clinical impacts and molecular mechanisms. Drug Resist Updates 5(2):88–114
• Gravina HD, Tafuri NF, Silva Ju´nior A, Fietto JLR, Oliveira TT, Diaz MAN, Almeida MR (2011) In vitro assessment of the antiviral potential of trans-cinnamic acid, quercetin and morin against equid herpesvirus1. Res Veterinary Sci 91(3):e158–e162
• Harborne JB, Williams CA (2000) Advances in flavonoid research since 1992. Phytochemistry 55:481–504
• Harriott MM, Noverr MC (2009) Candida albicans and Staphylococcus aureus from polymicrobial biofilms: effects on antimicrobial resistance. Antimicrob Agents Chemother 56(9): 3914–3922
• Haslam E (1996) Natural polyphenols (vegetable tannins) as drugs: possible modes of action. J Nat Prod 59:205–215
• Hatano T, Kusuda M, Inada K, Ogawa T, Shiota S, Tsuchiya T, Yoshida T (2005) Effects of tannins and related polyphenols on methicillin-resistant Staphylococcus aureus. Phytochemistry 66:2047–2055
• ICH (2005) Validation of Analytical Procedures: Text and Methodology Q2 (R1). Available online: http://www.ich.org accessed (accessed on 24 April 2012)
• Jabra-Rizk MA, Meiller TF, James CE, Shirtliff ME (2006) Effect of farnesol on Staphylococcus aureus biofilm formation and antimicrobial susceptibility. Antimicrob Agents Chemother 50(4):1463–1469
• Ji-Dong Gu, Brook B, Ralph M (2001) Protection of catheter surfaces from adhesion of Pseudomonas aeruginosa by a combination of silver ions and lectins. World J Microbiol & Biotechnol 17(2):173–179
• Khan MTH, Ather A, Thompson KD, Gambari R (2005) Extracts and molecules from medicinal plants against herpes simplex viruses. Antivir Res 67(2):107–119
• Kite P, Eastwood K, Sugden S, Percival SL (2004) Use of in vivogenerated biofilms from hemodialysis catheters to test the efficacy of a novel antimicrobial catheter lock for biofilm eradication in vitro. J Clin Microbiol 42(7):3073–3076
• Kratz JM, Andrighetti-Fröhner CR, Kolling DJ, Leal PC, Cirne-Santos CC, Yunes RA, Nunes RJ, Trybala E, Bergstrom T, Frugulhetti IC, Barardi CRM, Simo˜es CMO (2008) Anti-HSV-1 and anti-HIV-1 activity of gallic acid and pentyl gallate. Mem Inst Oswaldo Cruz 103(5):437–442
• Lückemeyer DD, Müller VDM, Moritz MIG, Stoco PH, Schenkel EP, Barardi CRM, Reginatto FH, Simöes CMO (2012) Effects of Ilex paraguariensis A. St. Hil. (Yerba Mate) on Herpes Simplex Virus Types 1 and 2 Replication. Phytother Res 26(4):535–540
• Maldaner G, Marangon P, Ilha V, Caro MSB, Burrow RA, Dalcol II, Morel AF (2011) Cyclopeptide alkaloids from Scutia buxifolia Reiss. Phytochemistry 72:804–809
• Merritt JH, Kadouri DE, O’Toole GA (2005) Growing and analyzing static biofilms. Curr Protoc Microbiol Chapter 1: Unit 1B.1 Morel FA, Maldaner G, Ilha V, Missau F, Silva FU, Dalcol I (2005) Cyclopeptide alkaloids from Scutia buxifolia Reiss and their antimicrobial activity. Phytochemistry 66:2571–2576
• Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63
• National Committee for Clinical Laboratory Standards (2002) Reference method for broth dilution antifungal susceptibility testing of yeast: approved standard, document M27–A2 Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: document M7–A5. National Comittee for Clinical Laboratory Standards, Wayne
• Oliveira I, Souza A, Valentäo P, Andrade PB, Ferreira ICRF, Ferreres F, Bento A, Seabra R, Estevinho L, Pereira JA (2007) Hazel (Corylus avellana L.) leaves as source of antimicrobial and antioxidative compounds. Food Chem 105:1018–1025
• Pellet PE, Roizman B (2007) The family Herpesviridae: a brief introduction. In: Knipe DM, Howley PM (eds) Fields virology. Lippincott Williams & Wilkins, Philadelphia, pp 2479–2499
• Peng SC, Cheng CY, Sheu F, Su CH (2008) The antimicrobial activity of heyneanol A extracted from the root of Taiwanese wild grape. J Appl Microbiol 105:485–491
• Pereira JA, Pereira APG, Ferreira ICFR, Valentäo P, Andrade PB, Seabra R, Estevinho L, Bento A (2006) Table olives from Portugal: phenolics compounds, antioxidant potential and antimicrobial activity. J Agr Food Chem 54:8425–8431
• Rangasamy O, Raoelison G, Rakotoniriana FE, Cheuk K, Urverg-Ratsimamanga S, Quetin-Leclercq J, Gurib-Fakim A, Subratty AH (2007) Screening for anti-infective properties of several medicinal plants of the Mauritians flora. J Ethnopharmacol 109:331–337
• Reed LJ, Muench H (1938) A simple method of estimating fifty per cent endpoints. Am J Hyg 27:493–494
• Simöes CMO, Falkenberg M, Mentz LA, Schenkel EP, Amoros M, Girre L (1999) Antiviral activity of south Brazilian medicinal plant extracts. Phytomedicine 6:205–214
• Soberón JR, Sgariglia MA, Quiroga EN, Vattuone MA (2007) Antibacterial activity of plant extracts from northwestern Argentina. J Appl Microbiol 102:1450–1461
• Stewart PS, Costerton JW (2001) Antibiotic resistance of bacteria in biofilms. Lancet 358:135–138
• Suárez B, Álvarez AL, Garcıa YD, Barrio GD, Lobo AP, Parra F (2010) Phenolic profiles, antioxidant activity and in vitro antiviral properties of apple pomace. Food Chem 120(1): 339–342
• Trevisan G, Maldaner G, Velloso NA, Sant’Anna GS, Ilha V, Gewehr CCV, Rubin MA, Morel AM, Ferreira J (2009) Antinociceptive effects of 14-membered cyclopeptide alkaloids. J Nat Prod 72:608–612
• Uozaki M, Yamasaki H, Katsuyama Y, Higuchi M, Higuti T, Koyama H (2007) Antiviral effect of octyl gallate against DNA and RNA viruses. Antivir Res 73:85–91
• Vanden Berghe DA, Vlietinck AJ, Van Hoof L (1986) Plant products as potential antiviral agents. Bull Inst Pasteur 84:101–147
• Wasicky R, Wasicky M, Joachimovits R (1964) Erstuntersuchungen und Coronilha Scutia buxifolia Reissek. Planta Med 12:13–25

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