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2013 | 35 | 07 |
Tytuł artykułu

Antimicrobial and antiviral activity-guided fractionation from Scutia buxifolia Reissek extracts

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Antimicrobial and antiviral activities of the fractions from Scutia buxifolia stem bark and leaves were evaluated. Best antimicrobial results occurred with the ethyl acetate (EA) and n-butanolic (NB) fractions from the leaves against Micrococcus sp. (minimal inhibitory concentration— MIC = 62.5 μg/ml), and NB fraction from stem bark and leaves against Klebsiella pneumoniae and Enterococcus faecalis (MIC = 62.5 μg/ml). The most active fractions were selected and fractioned into silica column to perform an in vitro antibiofilm assay, which evidenced subfractions EA2 and EA3 as the more active against Candida albicans (biofilm inhibitory concentration— BIC = 582 ± 0.01 lg/ml) and Staphylococcus aureus (BIC = 360 ± 0.007 lg/ml), respectively. The NB (selectivity index—SI = 25.78) and the EA (SI = 15.97) fractions from the stem bark, and the EA (SI = 14.13) fraction from the leaves exhibited a potential antiviral activity towards Herpes Simplex Virus type 1 whereas EA2 and EA3 subfractions from leaves (SI = 12.59 and 10.06, respectively), and NB2 subfraction from stem bark (SI = 12.34) maintained this good activity. Phenolic acids and flavonoids (gallic acid, chlorogenic acid, caffeic acid, rutin, isoquercitrin, quercitrin and quercetin) were identified by HPLC and may be partially responsible for the antimicrobial and antiherpes activities observed. The results obtained in this study showed that Scutia buxifolia has antibiofilm and anti-herpetic activities and that these properties are reported for the first time for this species.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
35
Numer
07
Opis fizyczny
p.2229-2239,fig.,ref.
Twórcy
autor
  • Laborato´rio de Fitoquı´mica, Departamento de Farma´cia Industrial, UFSM, Universidade Federal de Santa Maria, Campus Camobi, pre´dio 26, sala 1115, Santa Maria, RS 97105-900, Brazil
autor
  • Laborato´rio de Virologia, Departamento de Medicina Veterina´ria Preventiva, UFSM, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil
autor
  • Laborato´rio de Pesquisas Micolo´gicas, Departamento de Microbiologia e Parasitologia da UFSM, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazi
  • Laborato´rio de Fitoquı´mica, Departamento de Farma´cia Industrial, UFSM, Universidade Federal de Santa Maria, Campus Camobi, pre´dio 26, sala 1115, Santa Maria, RS 97105-900, Brazil
autor
  • Laborato´rio de Fitoquı´mica, Departamento de Farma´cia Industrial, UFSM, Universidade Federal de Santa Maria, Campus Camobi, pre´dio 26, sala 1115, Santa Maria, RS 97105-900, Brazil
autor
  • Laborato´rio de Virologia, Departamento de Medicina Veterina´ria Preventiva, UFSM, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil
autor
  • Laborato´rio de Virologia, Departamento de Medicina Veterina´ria Preventiva, UFSM, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil
autor
  • Laborato´rio de Pesquisas Micolo´gicas, Departamento de Microbiologia e Parasitologia da UFSM, Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazi
  • Laborato´rio de Microbiologia Clı´nica, Cieˆncias da Sau´de, UNIFRA, Centro Universita´rio Franciscano, Santa Maria, RS 97010-032, Brazil
  • Laborato´rio de Microbiologia Clı´nica, Cieˆncias da Sau´de, UNIFRA, Centro Universita´rio Franciscano, Santa Maria, RS 97010-032, Brazil
  • Laborato´rio de Fitoquı´mica, Departamento de Farma´cia Industrial, UFSM, Universidade Federal de Santa Maria, Campus Camobi, pre´dio 26, sala 1115, Santa Maria, RS 97105-900, Brazil
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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