Identification of changes in Triticum aestivum L. leaf proteome in response to drought stress by 2D-PAGE and MALDI-TOF/TOF mass spectrometry

• Autorzy: Zhang H. , Zhang L. , Lv H. , Yu Z. , Zhang D. , Zhu W.
• Języki publikacji: EN

Abstrakty

EN

Drought is an abiotic stress that strongly influences plant growth, development and productivity. To gain a better understanding of the drought-stress responses at physiological and molecular level in wheat plants (Triticum aestivum cv. KTC86211), we performed a comparative physiological and proteomics analysis. Eight-day-old wheat seedlings were treated with polyethylene glycolsimulated drought stress for 0, 24, 48 and 72 h. Drought treatment resulted in alterations of morphology, increased relative electrolyte leakage and reduced length and weight on leaf and root. Stress-induced proteome changes were analyzed by two-dimensional gel electrophoresis in conjunction with MALDI-TOF/TOF. Twenty-three spots differed significantly between control and treated plants following 48 h of drought stress, with 19 upregulated, and 4 downregulated, in leaf tissues. All of the differentially expressed protein spots were identified, revealing that the majority of proteins altered by drought treatment were involved in reactive oxygen species scavenging enzymes and photosynthesis. Other proteins identified were involved in protein metabolism, cytoskeleton structure, defense response, acid metabolism and signal transduction. All proteins might contribute cooperatively to reestablish cellular homeostasis under drought stress. The present study not only provides new insights into the mechanisms of acclimation and tolerance to drought stress in wheat plants, but also provides clues for improving wheat’s drought tolerance through breeding or genetic engineering.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 06
• Opis fizyczny: p.1385-1398,fig.,ref.

Twórcy

autor

Zhang H.

• State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A and F University, Yangling, Shaanxi, People's Republic of China
• College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, People's Republic of China

autor

Zhang L.

• State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A and F University, Yangling, Shaanxi, People's Republic of China

autor

Lv H.

• State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A and F University, Yangling, Shaanxi, People's Republic of China

autor

Yu Z.

• State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A and F University, Yangling, Shaanxi, People's Republic of China

autor

Zhang D.

• State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A and F University, Yangling, Shaanxi, People's Republic of China

autor

Zhu W.

• State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A and F University, Yangling, Shaanxi, People's Republic of China

Bibliografia

• Abdrakhamanova A, Wang Q, Khokhlova L, Nick P (2003) Is microtubule disassembly a trigger for cold acclimation? Plant Cell Physiol 44:676–686
• Alam I, Lee DG, Kim KH, Park CH, Sharmin SA, Lee H et al (2010) Proteome analysis of soybean roots under waterlogging stress at an early vegetative stage. J Biosci 35:49–62
• Aranjuelo I, Molero G, Erice G, Avice JC, Nogués S (2011) Plant physiology and proteomics reveals the leaf response to drought in alfalfa (Medicago sativa L.). J Exp Bot 62:111–123
• Ashraf M, Harrisb PJC (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci 166:3–16
• Bazargani MM, Sarhadi E, Bushehri AS, Matros A, Mock HP, Naghavi M et al (2011) A proteomics view on the role of drought-induced senescence and oxidative stress defense in enhanced stem reserves remobilization in wheat. J Proteomics 74:1959–1973
• Bhushan D, Pandey A, Choudhary MK, Datta A, Chakraborty S, Chakraborty N (2007) Comparative proteomics analysis of differentially expressed proteins in chickpea extracellular matrix during dehydration stress. Mol Cell Proteomics 6:1868–1884
• Bogeat-Triboulot MB, Brosché M, Renaut J, Jouve L, Le TD, Fayyaz P et al (2007) Gradual soil water depletion results in reversible changes of gene expression, protein profiles, ecophysiology, and growth performance in Populus euphratica, a poplar growing in arid regions. Plant Physiol 143:876–892
• Bradford MM (1976) A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
• Brenchley R, Spannagl M, Pfeifer M, Barker GLA, D’Amore R, Allen AM et al (2012) Analysis of the bread wheat genome using whole-genome shotgun sequencing. Nature 49:705–710
• Briat JF, Ravet K, Arnaud N, Duc C, Boucherez J, Touraine B, Cellier F, Gaymard F (2010) New insights into ferritin synthesis and function highlight a link between iron homeostasis and oxidative stress in plants. Ann Bot 105:811–822
• Candiano G, Bruschi M, Musante L, Santucci L, Ghiggeri GM, Carnemolla B, Orecchia P, Zardi L, Righetti PG (2004) Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25:1327–1333
• Cao WH, Liu J, He XJ, Mu RL, Zhou HL, Chen SY et al (2007) Modulation of ethylene responses affects plant salt-stress responses. Plant Physiol 143:707–719
• Caruso G, Cavaliere C, Guarino C, Gubbiotti R, Foglia P, Laganà A (2008) Identification of changes in Triticum durum L. leaf proteome in response to salt stress by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Anal Bioanal Chem 391:381–390
• Caruso G, Cavaliere C, Foglia P, Gubbiotti R, Samperi R, Lagana A (2009) Analysis of drought responsive proteins in wheat (Triticum durum) by 2D-PAGE and MALDI-TOF mass spectrometry. Plant Sci 177:570–576
• Chaves MM, Flexas J, Pinheiro C (2009) Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann Bot 103:551–560
• Cheng L, Gao X, Li S, Shi M, Javeed H, Jing X et al (2010) Proteomic analysis of soybean [Glycine max (L.) Meer.] seeds during imbibition at chilling temperature. Mol Breed 26:1–17
• Chinnusamy V, Schumaker K, Zhu JK (2004) Molecular genetic perspectives on crosstalk and specificity in abiotic stress signalling in plants. J Exp Bot 55:225–236
• Chivasa S, Ndimba BK, Simon WJ, Robertson D, Yu XL, Knox JP et al (2002) Proteomic analysis of the Arabidopsis thaliana cell wall. Electrophoresis 23:1754–1765
• Cramer GR, Ergül A, Grimplet J, Tillett RL, Tattersall EAR, Bohlman MC et al (2007) Water and salinity stress in grapevines: early and late changes in transcript and metabolite profiles. Funct Integr Genomics 7:111–134
• Damerval C, Vienne DD, Zivy M, Thiellement H (1986) Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophoresis 7:52–54
• Ermolayev V, Weschke W, Manteuffel R (2003) Comparison of Al-induced gene expression in sensitive and tolerant soybean cultivars. J Exp Bot 54:2745–2756
• Foyer CH, Lelandais M, Kunert KJ (1994) Photooxidative stress in plants. Physiol Plant 92:696–717
• Gao F, Zhou Y, Zhu W, Li X, Fan LM, Zhang G (2009) Proteomic analysis of cold stress-responsive proteins in Thellungiella rosette leaves. Planta 230:1033–1046
• García-Lorenzo M (2007) The role of proteases in plant development. Dissertation, Umeå University
• Gazanchian A, Hajheidari M, Sima NK, Salekdeh GH (2007) Proteome response of Elymus elongatum to severe water stress and recovery. J Exp Bot 58:291–300
• Hajheidari M, Eivazi A, Buchanan BB, Wong JH, Majidi I, Salekdeh GH (2007) Proteomics uncovers a role for redox in drought tolerance in wheat. J Proteome Res 6:1451–1460
• Harb A, Krishnan A, Ambavaram MMR, Pereira A (2010) Molecular and physiological analysis of drought stress in Arabidopsis reveals early responses leading to acclimation in plant growth. Plant Physiol 154:1254–1271
• Hernández JA, Olmos E, Corpas FJ, Sevilla F, de1 Rio LA (1995) Salt-induced oxidative stress in chloroplasts of pea plants. Plant Sci 105:151–167
• Hoagland DR, Arnon DI (1938) The water-culture method for growing plants without soil. Calif Agric Exp Stn Circ 347:1–39
• Jacquot JP, Lancelin JM, Meyer Y (1997) Thioredoxins: structure and function in plant cells. New Phytol 136:543–570
• Jiang Y, Yang B, Harris NS, Deyholos MK (2007) Comparative proteomic analysis of NaCl stress-responsive proteins in Arabidopsis roots. J Exp Bot 58:3591–3607
• Jiang S, Liang X, Li X, Wang S, Lv D, Ma C, Li X, Ma W, Yan Y (2012) Wheat drought-responsive grain proteome analysis by linear and nonlinear 2-DE and MALDI-TOF mass spectrometry. Int J Mol Sci 13:16065–16083
• Jorge I, Navarro RM, Lenz C, Ariza D, Jorrín J (2006) Variation in the holm oak leaf proteome at different plant developmental stages, between provenances and in response to drought stress. Proteomics 6:207–214
• Kamal AHM, Cho K, Choi JS, Bae KH, Komatsu S, Uozumi N, Woo SH (2013) The wheat chloroplastic proteome. J Proteomics 93:326–342
• Kim KY, Park SW, Chung YS, Chung CH, Kim JI, Lee JH (2004) Molecular cloning of low-temperature-inducible ribosomal proteins from soybean. J Exp Bot 55:1153–1155
• Kingston-Smith AH, Foyer CH (2000) Bundle sheath proteins are more sensitive to oxidative damage than those of the mesophyll in maize leaves exposed to paraquat or low temperatures. J Exp Bot 51:123–130
• Kmiec B, Glaser E (2012) A novel mitochondrial and chloroplast peptidasome, PreP. Physiol Plant 145:180–186
• Kosmala A, Bocian A, Rapacz M, Jurczyk B, Zwierzykowski Z (2009) Identification of leaf proteins differentially accumulated during cold acclimation between Festuca pratensis plants with distinct levels of frost tolerance. J Exp Bot 60:3595–3609
• Kosová K, Vítámvás P, Práši IT, Renaut J (2011) Plant proteome changes under abiotic stress-contribution of proteomics studies to understanding plant stress response. J Proteomics 74:1301–1322
• Lee DG, Ahsan N, Lee SH, Kang KY, Bahk JD, Lee IJ et al (2007) A proteomic approach in analyzing heat-responsive proteins in rice leaves. Proteomics 7:3369–3383
• Lu DB, Sears RG, Paulsen GM (1989) Increasing stress resistance by in vitro selection for abscisic acid insensitivity in wheat. Crop Sci 29:939–943
• Luan S, Kudla J, Rodriguez-Concepcion M, Yalovsky S, Gruissem W (2002) Calmodulins and calcineurin B-like proteins: calcium sensors for specific signal response coupling in plants. Plant Cell 14:S389–S400
• Manaa A, Ahmed HB, Smiti S, Faurobert M (2011) Salt-stress induced physiological and proteomic changes in Tomato (Solanum lycopersicum) seedlings. OMICS 15:801–809
• McDowell NG (2011) Mechanisms linking drought, hydraulics, carbon metabolism, and vegetation mortality. Plant Physiol 155:1051–1059
• Noutoshi Y, Ito T, Seki M, Nakashita H, Yoshida S, Marco Y, Shirasu K, Shinozaki K (2005) A single amino acid insertion in the WRKY domain of the Arabidopsis TIR-NBS-LRR-WRKY-type disease resistance protein SLH1 (sensitive to low humidity 1) causes activation of defense responses and hypersensitive cell death. Plant J 43:873–888
• O’Farrel PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021
• Parker R, Flowers TJ, Moore AL, Harpham NV (2006) An accurate and reproducible method for proteome profiling of the effects of salt stress in the rice leaf lamina. J Exp Bot 57:1109–1118
• Parry MA, Andralojc PJ, Mitchell RA, Madgwick PJ, Keys AJ (2003) Manipulation of Rubisco: the amount, activity, function and regulation. J Exp Bot 54:1321–1333
• Peng Z, Wang M, Li F, Lv H, Li C, Xia G (2009) A proteomic study of the response to salinity and drought stress in an introgression strain of bread wheat. Mol Cell Proteomics 8:2676–2686
• Pereira S, Pissarra J, Sunkel C, Salema R (1996) Tissue-specific distribution of glutamine synthetase in potato tubers. Ann Bot 77:429–432
• Plomion C, Lalanne C, Claverol S, Meddour H, Kohler A, Bogeat-Triboulot MB et al (2006) Mapping the proteome of poplar and application to the discovery of drought stress responsive proteins. Proteomics 6:6509–6527
• Portis AR Jr (2003) Rubisco activase—Rubisco’s catalytic chaperone. Photosynth Res 751:11–27
• Pradet-Balade B, Boulme F, Beug H, Mullner EW, Garcia-Sanz JA (2001) Translation control: bridging the gap between genomics and proteomics? Trends Biochem Sci 26:225–229
• Ravet K, Touraine B, Boucherez J, Briat JF, Gaymard F, Cellier F (2009) Ferritins control interaction between iron homeostasis and oxidative stress in Arabidopsis. Plant J 57:400–412
• Rey P, Pruvot G, Becuwe N, Eymery F, Rumeau D, Peltier G (1998) A novel thioredoxin-like protein located in the chloroplast is induced by water deficit in Solanum tuberosum L. plants. Plant J 13:97–107
• Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett J (2002) Proteomic analysis of rice leaves during drought stress and recovery. Proteomics 2:1131–1145
• Schroda M, Vallon O, Wollman FA, Beck CF (1999) A chloroplast-targeted heat shock protein 70 (HSP70) contributes to the photoprotection and repair of photosystem II during and after photoinhibition. Plant Cell 11:1165–1178
• Smalle J, Vierstra RD (2004) The ubiquitin 26S proteasome proteolytic pathway. Annu Rev Plant Biol 55:555–590
• Taylor NL, Heazlewood JL, Day DA, Millar AH (2005) Differential impact of environmental stresses on the pea mitochondrial proteome. Mol Cell Proteomics 4:1122–1133
• Thomashow MF (2001) So what’s new in the field of plant cold acclimation? Lots! Plant Physiol 125:89–93
• Toorchi M, Yukawa K, Nouri MZ, Komatsu S (2009) Proteomics approach for identifying osmotic-stress-related proteins in soybean roots. Peptides 30:2108–2117
• Uribe R, Jay D (2009) A review of actin binding proteins: new perspectives. Mol Biol Rep 36:121–125
• Veljovic-Jovanovic S, Kukavica B, Stevanovic B, Navari-Izzo F (2006) Senescence- and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica. J Exp Bot 57:1759–1768
• Vincent D, Lapierre C, Pollet B, Cornic G, Negroni L, Zivy M (2005) Water deficits affect caffeate O-methyltransferase, lignification, and related enzymes in maize leaves: a proteomic investigation. Plant Physiol 137:949–960
• Vincent D, Ergul A, Bohlman MC, Tattersall EAR, Tillett RL, Wheatley MD et al (2007) Proteomic analysis reveals differences between Vitis vinifera L. cv. Chardonnay and cv. Cabernet Sauvignon and their responses to water deficit and salinity. J Exp Bot 58:1873–1892
• Wang W, Vinocur B, Shoseyov O, Altman A (2004) Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci 9:244–252
• Waraich EA, Ahmad R, Saifullah Ashraf MY, Ehsanullah (2011) Role of mineral nutrition in alleviation of drought stress in plants. Aust J Crop Sci 5:764–777
• Xiao X, Yang F, Zhang S, Korpelainenc H, Li C (2009) Physiological and proteomic responses of two contrasting Populus cathayana populations to drought stress. Physiol Plant 136:150–168
• Yamaguchi M, Valliyodan B, Zhang J, Lenoble ME, Yu O, Rogers EE, Nguyen HT, Sharp RE (2010) Regulation of growth response to water stress in the soybean primary root. I. Proteomic analysis reveals region-specific regulation of phenylpropanoid metabolism and control of free iron in the elongation zone. Plant Cell Environ 33:223–224
• Yamori W, Takahashi S, Makino A, Price GD (2011) The roles of ATP synthase and the cytochrome b6/f complexes in limiting chloroplast electron transport and determining photosynthetic capacity. Plant Physiol 155:956–962
• Yan SP, Zhang QY, Tang ZC, Su WA, Sun WN (2006) Comparative proteomic analysis provides new insights into chilling stress responses in rice. Mol Cell Proteomics 5:484–496
• Yang F, Wang Y, Miao L (2010) Comparative physiological and proteomic responses to drought stress in two poplar species originating from different altitudes. Physiol Plant 139:388–400
• Yang F, Jørgensen AD, Li H, Søndergaard I, Finnie C, Svensson B, Jiang D, Wollenweber B, Jacobsen S (2011) Implications of high-temperature events and water deficits on protein profiles in wheat (Triticum aestivum L. cv. Vinjett) grain. Proteomics 11:1684–1695
• Ye Z, Rodriguez R, Tran A, Hoang H, Santos D, Brown S, Vellanoweth R (2000) The developmental transition to flowering represses ascorbate peroxidase activity and induces enzymatic lipid peroxidation in leaf tissue in Arabidopsis thaliana. Plant Sci 158:115–127
• Yoshimura K, Masuda A, Kuwano M, Yokota1 A, Akashi1 K (2008) Programmed proteome response for drought avoidance/tolerance in the root of a C3 xerophyte (wild watermelon) under water deficits. Plant Cell Physiol 49:226–241
• Zhang J, Kirkham MB (1994) Drought-stress-induced changes in activities of superoxide dismutase, catalase, and peroxidase in wheat species. Plant Cell Physiol 35:785–791

Identyfikatory

DOI

10.1007/s11738-014-1517-9