PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Czasopismo

2014 | 59 | 4 |

Tytuł artykułu

Genetic characterization of Toxoplasma gondii isolates from chickens in India by GRA6 gene sequence analysis

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
PCR-RFLP and nucleotide sequencing based genotyping of Toxoplasma gondii Indian isolates (Izatnagar and Chennai isolates and Chennai clone) vis-a vis RH-IVRI strain was conducted by targeting GRA6 as genetic marker. The 791 bp GRA6 product was PCR amplified from the genomic DNA of different T. gondii Indian isolates, including the RH-IVRI strain. Tru1I restriction endonuclease based PCR-RFLP of GRA6 sequence produced polymorphic digestion pattern that discriminated the virulent RH-IVRI strain (as type I) from the moderately virulent local isolates as type III. The PCR amplicon of T. gondii GRA6 from RH-IVRI strain as well as from the local isolates were cloned in cloning vector and custom sequenced. The nucleotide and deduced amino acid sequences of T. gondii isolates were aligned with that of the type I, II and III strains (RH, BEVERLEY, ME49, C56, TONT and NED) available in public domain and analyzed in silico using MEGA version 4.0 software. Nucleotide sequencing and phylogenetic analysis of GRA6 marker from the Indian isolates revealed a close genetic relationship with type III strains of T. gondii. Further, detection of a single nucleotide polymorphism (SNP) at positions 162 and 171 of the GRA6 marker, established the lineage of Indian isolates as type III. This is the first report on characterization of T. gondii lineage as type III in selective chicken population of India based on PCR-RFLP and sequence analysis of GRA6 gene.

Wydawca

-

Czasopismo

Rocznik

Tom

59

Numer

4

Opis fizyczny

p.666-674,fig.,ref.

Twórcy

autor
  • Veterinary Dispensary, Kudugi, Karnataka, 586121, India
  • Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, India
autor
  • Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, India
autor
  • Post Graduate Research Institute in Animal Sciences, Kattupakkam, 603203, India
autor
  • Division of Temperate Animal Husbandry, Mukteswsar 263138, India
  • Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, India

Bibliografia

  • Ajzenberg D., Banuls A.L., Su C., Dumetre A., Demar M., Carme B., Darde M.L. 2004. Genetic diversity, clonality and sexuality in Toxoplasma gondii. International Journal for Parasitology, 34, 1185–1196. DOI: http://dx.doi.org/ 10.1016/j.ijpara.2004.06.007.
  • Ajzenberg D., Banuls A.L., Tibayrenc M., Darde M.L. 2002a. Microsatellite analysis of Toxoplasma gondii shows considerable polymorphism structured into two main clonal groups. International Journal for Parasitology, 32, 27–38. DOI: http://dx.doi.org/ 10.1016/S0020-7519(01)00301-0.
  • Ajzenberg D., Cogne N., Paris L., Bessieres M.H., Thulliez P., Filisetti D., Pelloux H., Marty P., Darde M.L. 2002b. Genotype of 86 Toxoplasma gondii isolates associated with human congenital toxoplasmosis, and correlation with clinical findings. Journal of Infectious Diseases, 186, 684–689. DOI: http://dx.doi.org/ 10.1086/342663.
  • Belfort-Neto R., Nussenblatt V., Rizzo L., Mucciolo C., Silveira C., Nussenblatt R., Khan A., Sibley L.D., Belfort R. 2007. High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, southern Brazil. Anais da Academia Brasileira de Ciências, 79, 111–114. DOI: http://dx.doi.org/ 10.1590/S0001-37652007000100013.
  • Cesbron-Delauw M.F. 1994. Dense granule organelles of Toxoplasma gondii: Their role in the host-parasite relationship. Parasitology Today, 10, 293–296. DOI: http://dx.doi.org/ 10.1016/0169-4758(94)90078-7.
  • Coppens I., Andries M., Liu J.L., Cesbron-Delauw M.F. 1999. Intracellular trafficking of dense granule proteins in Toxoplasma gondii and experimental evidences for a regulated exocytosis. European Journal of Cell Biology, 78, 463–474. DOI.: http://dx.doi.org/ 10.1016/S0171-9335(99)80073-9.
  • Costa J.M., Darde M.L., Assoline B., Vidaud M., Bretagne S. 1997. Microsatellite in the Beta-Tubulin gene of Toxoplasma gondii as a new genetic marker for use in direct screening of amniotic fluids. Journal of Clinical Microbiology, 35, 2542–2545.
  • Darde M.L., Bouteille B., Pestre-Alexandre M. 1992. Isoenzyme analysis of 35 Toxoplasma gondii isolates and the biological and epidemiological implications. Journal of Parasitology, 78, 786–794. DOI: http://dx.doi.org/ 10.2307/3283305.
  • Decoster A., Darcy F., Capron A. 1988. Recognition of Toxoplasma gondii-excreted and secreted antigens by human sera from acquired and congenital toxoplasmosis: identification of markers of acute and chronic infection. Clinical and Experimental Immunology, 73, 76–82.
  • Dhumne M., Sengupta C., Kadival G., Rathinaswamy A., Velumani A. 2007. National seroprevalence of Toxoplasma gondii in India. Journal of Parasitology, 93, 1520–1521. DOI: 10.1645/GE-1159.1.
  • Dubey J.P., Gennari S.M., Labruna M.B., Camargo L.M.A., Vianna M.C.B., Marcet P.L., Lehmann T. 2006a. Characterization of Toxoplasma gondii isolates in free-range chickens from Amazon, Brazil. Journal of Parasitology, 92, 36–40. DOI: http://dx.doi.org/ 10.1645/GE-655R.1.
  • Dubey J.P., Applewhaite L., Sundar N., Velmurugan G.V., Bandini L.A., Kwok O.C., Hill R., Su C. 2007a. Molecular and biological characterization of Toxoplasma gondii isolates from free-range chickens from Guyana, South America, identified several unique and common parasite genotypes. Parasitology, 18, 1–7. DOI: http://dx.doi.org/ 10.1017/S0031182007003083.
  • Dubey J.P., Beattie C.P. 1988. Toxoplasmosis of Animals and Man. CRC Press, Boca Raton, Florida.
  • Dubey J.P. (1998). Toxoplasmosis, sarcocystosis, isosporosis, and cyclosporosis. In: Palmer S.R., Soulsby E.J.L., Simpson D.J.H., (Ed). Zoonoses. Oxford: Oxford University Press, 579–597.
  • Dubey J.P., Huong L.T.T., Sundar N., Su C. 2007b. Genetic characterization of Toxoplasma gondii isolates in dogs from Vietnam suggests their South American origin. Veterinary Parasitology, 146, 347–351. DOI:10.1016/j.vetpar.2007.03.008.
  • Dubey J.P., Rajendran C., Ferreira L.R., Martins J., Kwok O.C.H., Hill D.E., Villena I., Zhou H., Su C., Jones J.L. 2011. High prevalence and genotypes of Toxoplasma gondii isolated from goats, from a retail meat store, destined for human consumption in the USA. International Journal of Parasitology, 41, 827–833. DOI:10.1016/j.ijpara.2011.03.006.
  • Dubey J.P., Su C., Oliveira J., Morales J.A., Bolanos R.V., Sundar N., Kwok O.C.H. Shen S.K. 2006b. Biological and genetic characterization of Toxoplasma gondii isolates in freerange chickens from Costa Rica, Central America. Veterinary Parasitology, 139, 29–36. DOI:10.1016/j.vetpar.2006.02.031.
  • Dubey J.P., Zhu X.Q., Sundar N., Zhang H., Kwok O.C., Su C. 2007c. Genetic and biologic characterization of Toxoplasma gondii isolates of cats from China. Veterinary Parasitology, 145, 352–356. DOI:10.1016/j.vetpar.2006.12.016.
  • Edvinsson B., Darde M.L., Pelloux H., Evengard B. 2007. Rapid genotyping of Toxoplasma gondii by pyrosequencing. Clinical Microbiology and Infection, 13, 424–429. DOI: http://dx.doi.org/ 10.1111/j.1469-0691.2006.01661.x.
  • Fazaeli A., Carter P.E., Darde M.L., Pennington T.H. 2000. Molecular typing of Toxoplasma gondii strains by GRA6 gene sequence analysis. International Journal for Parasitology, 30, 637–642. DOI: http://dx.doi.org/ 10.1016/S0020-7519(00)00036-9.
  • Gross U., Muller W.A., Knapp S., Heesemann J. 1991. Identification of a virulence-associated antigen of Toxoplasma gondii by a mouse monoclonal antibody. Infection and Immunity, 59, 4511–4516.
  • Hill D., Dubey J.P. 2002. Toxoplasma gondii: Transmission, diagnosis and prevention. Clinical Microbiology and Infection, 8, 634–640. DOI: http://dx.doi.org/ 10.1046/j.1469-0691.2002.00485.x.
  • Howe D.K., Honore S., Derouin F., Sibley L.D. 1997. Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis. Journal of Clinical Microbiology, 35, 1411–1414.
  • Howe D.K., Sibley L.D. 1995. Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease. Journal of Infectious Diseases, 172, 1561–1566. DOI: http://dx.doi.org/ 10.1093/infdis/172.6.1561.
  • Jungersen G., Jensen L., Rask M.R., Lind P. 2002. Non-lethal infection parameters in mice separate sheep Type II Toxoplasma gondii isolates by virulence. Comparative Immunology, Microbiology and Infectious Diseases, 25, 187–195. DOI: http://dx.doi.org/ 10.1016/S0147-9571(01)00039-X.
  • Khan A., Dubey J.P., Su C., Ajioka J.W., Rosenthal B.M., Sibley L.D. 2011 Genetic analyses of atypical Toxoplasma gondii strains reveal a fourth clonal lineage in North America. International Journal of Parasitology, 41:645–655. DOI: 10.1016/j.ijpara.2011.01.005.
  • Khan A., Jordan C., Muccioli C., Vallochi A.L., Rizzo L.V., Belfort Jr. R., Vitor R. W.A., Silveira C., Sibley L. D. 2006. Genetic divergence of Toxoplasma gondii strains associated with ocular Toxoplasmosis, Brazil. Emerging Infectious Diseases, 12, 942–949. DOI: 10.3201/eid1206.060025.
  • Khan A., Su C., German M., Storch G.A., Clifford D.B., Sibley L.D. 2005. Genotyping of Toxoplasma gondii strains from immunocompromised patients reveals high prevalence of type I strains. Journal of Clinical Microbiology, 43, 5881–5887. DOI: 10.1128/JCM.43.12.5881-5887.2005.
  • Lecordier L., Moleon-Borodowsky I., Dubremetz J.F., Tourvieille B., Mercier C., Deslee D., Capron A., Cesbron-Delauw. 1995. Characterization of a dense granule antigen of Toxoplasma gondii (GRA6) associated to the network of the parasitophorous vacuole. Molecular and Biochemical Parasitology, 70, 85–94. DOI: http://dx.doi.org/ 10.1016/0166-6851(95)00010-X.
  • Lehmann T., Marcet P.L., Graham D.H., Dahl E.R., Dubey J.P. 2006. Globalization and the population structure of Toxoplasma gondii. Proceedings of the National Academy of Sciences of the United States of America, 103, 11423–11428. DOI: 10.1073/pnas.0601438103.
  • Lin A., Shin E.H., Kim T.Y., Park J.H., Guk S.M., Chai J.Y. 2005. Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii. Korean Journal of Parasitology, 43, 27–32. DOI: http://dx.doi.org/ 10.3347/kjp.2005.43.1.27.
  • Miller M.A., Grigg M.E., Kreuder C., James E.R., Melli A.C., Crosbie P.R., Jessup D.A., Boothroyd J.C., Brownstein D., Conrad P.A. 2004. An unusual genotype of Toxoplasma gondii is common in California sea otters (Enhydra lutris nereis) and is associated with mortality. International Journal for Parasitology, 34, 275–284. DOI: http://dx.doi.org/ 10.1016/j.ijpara.2003.12.008.
  • Mondragon R., Howe D.K., Dubey J.P., Sibley L.D. 1998. Genotypic analysis of Toxoplasma gondii isolates from pigs. Journal of Parasitology, 84, 639–641. DOI: http://dx.doi.org/ 10.2307/3284743.
  • More G., Pardini L., Basso W., Machuca M., Bacigalupe D., Villanueva M.C., Schares G., Venturini M.C., Venturini L. 2010. Toxoplasmosis and genotyping of Toxoplasma gondii in Macropus rufus and Macropus giganteus in Argentina. Veterinary Parasitology, 169, 57–61. DOI: 10.1016/j.vetpar.2009.12.004.
  • Owen M.R., Trees A.J. 1999. Genotyping of Toxoplasma gondii associated with abortion in sheep. Journal of Parasitology, 85, 382–384. DOI: http://dx.doi.org/ 10.2307/3285654.
  • Pena H.F.J., Gennari S.M., Dubey J.P., Su C. 2008. Population structure and mouse-virulence of Toxoplasma gondii in Brazil. International Journal for Parasitology, 38, 561–569. DOI: 10.1016/j.ijpara.2007.09.004.
  • Petersen E., Edvinsson B., Lundgren B., Benfield T., Evengard B. 2006. Diagnosis of pulmonary infection with Toxoplasma gondii in immunocompromised HIV-positive patients by realtime PCR. European Journal of Clinical Microbiology & Infectious Diseases, 25, 401–404. DOI: 10.1007/s10096-006-0156-5.
  • Quan J.H., Kim T.Y., Choi I.U., Lee Y.H. 2008. Genotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis. Korean Journal of Parasitology, 46, 105–108. DOI: 10.3347/kjp.2008.46.2.105.
  • Ragozo A.M.A., Pena H.F.J., Yai L.E.O., Su C., Gennari S.M. 2010. Genetic diversity among Toxoplasma gondii isolates of small ruminants from Brazil: Novel genotypes revealed. Veterinary Parasitology, 170, 307–312. DOI: 10.1016/j.vetpar.2010.02.024.
  • Singh H., Tewari A.K., Mishra A.K., Maharana B.R., Rao J.R., Raina O.K. 2011a. Molecular cloning, comparative sequence analysis and prokaryotic expression of GRA5 protein of Toxoplasma gondii. Indian Journal of Animal Sciences, 81, 209–15.
  • Singh H., Tewari A.K., Mishra A.K., Maharana B.R., Rao J.R., Raina O.K. 2011b. Molecular cloning and comparative sequence analysis of open reading frame of SAG2 gene of Toxoplasma gondii. Journal of Veterinary Parasitology, 25, 107–112.
  • Smith D.D., Frenkel J.K. 2003. Immunological comparison of 124 isolates of Toxoplasma gondii. Parasitology Research, 91, 332–337. DOI: http://dx.doi.org/ 10.1007/s00436-003-0886-6.
  • Soaresa R.M., Silveira L.H., da Silva A.V., Ragozoa A., Galli S., Lopes E.G., Gennari S.M., Pena H.F.J. 2011. Genotyping of Toxoplasma gondii isolates from free range chickens in the Pantanal area of Brazil. Veterinary Parasitology, 178, 29–34. DOI: 10.1016/j.vetpar.2010.12.037.
  • Sousa S., Ajzenberg D., Marle M., Aubert D., Villena I., Costa J.C., Darde M. L. 2009. Selection of polymorphic peptides from GRA6 and GRA7 sequences of Toxoplasma gondii strains to be used in serotyping. Clinical and Vaccine Immunology, 16, 1158–1169. DOI: 10.1128/CVI.00092-09.
  • Sreekumar C. 2001. PCR based diagnosis and genotyping of Toxoplasma gondii. Ph.D. thesis, Deemed University, IVRI, Izatnagar, U.P. India.
  • Sreekumar C. Grahamb D.H. Dahl E., Lehmann T., Raman M., Bhalerao D.P., Vianna M.C.B., Dubey J.P. 2003. Genotyping of Toxoplasma gondii isolates from chickens from India. Veterinary Parasitology, 118, 187–194. DOI: http://dx.doi.org/ 10.1016/j.vetpar.2003.10.018.
  • Su C., Khan A., Zhou P., Majumdar D., Ajzenberg D., Dardé M.L., Zhu X.Q., Ajioka J.W., Rosenthal B.M., Dubey J.P., Sibley L.D. 2012. Globally diverse Toxoplasma gondii isolates comprise six major clades originating from a small number of distinct ancestral lineages. Proceedings of the National Academy of Sciences, 109, 5844–5849. DOI: 10.1073/pnas.1203190109.
  • Su C., Zhang X., Dubey J.P. 2006. Genotyping of Toxoplasma gondii by multilocus PCR-RFLP markers: A high resolution and simple method for identification of parasites. International Journal for Parasitology, 36, 841–848. DOI: 10.1016/j.ijpara.2006.03.003.
  • Sudan V., Tewari A.K., Singh H., Saravanan B.C., Sankar M. 2012. Molecular characterization of surface antigen 3 (SAG 3) gene of Toxoplasma gondii RH-IVRI strain. Journal of Parasitic Diseases, 36, 210–214. DOI: 10.1007/s12639-012-0107-2.
  • Tamura K., Dudley J., Nei M., Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599. DOI: 10.1093/molbev/msm092.
  • Tenter A.M., Heckeroth A.R., Weiss L.M. 2000. Toxoplasma gondii: From animal to humans. International Journal for Parasitology, 30, 1217–1258. DOI: http://dx.doi.org/ 10.1016/S0020-7519(00)00124-7.
  • Velmurugan G.V. Dubey J.P., Su C. 2008. Genotyping studies of Toxoplasma gondii isolates from Africa revealed that the archetypal clonal lineages predominate as in North America and Europe. Veterinary Parasitology, 155, 314–318. DOI: 10.1016/j.vetpar.2008.04.021.
  • Velmurugan G.V., Su C., Dubey J.P. 2009. Isolate designation and characterization of Toxoplasma gondii isolates from pigs in the United States. Journal of Parasitology, 95, 95–99. DOI: 10.1645/GE-1746.1.
  • Zakimi S., Kyan H., Oshiro M., Sugimoto C., Xuenan X., Fujisaki K. 2006. Genetic characterization of GRA6 genes from Toxoplasma gondii from pigs in Okinawa, Japan. Journal of Veterinary Medical Science, 68, 1105–1107. DOI: http://dx.doi.org/ 10.1292/jvms.68.1105.
  • Zhou P., Zhang H., Lin R.Q., Zhang D.L., Song H.Q., Su C., Zhu X.Q. 2009. Genetic characterization of Toxoplasma gondii isolates from China. Parasitology International, 58, 193–195. DOI: 10.1016/j.parint.2009.01.006.
  • Zia-Ali N., Fazaeli A., Khoramizadeh M., Ajzenberg D., Darde M., Keshavarz-Valian H. 2007. Isolation and molecular characterization of Toxoplasma gondii strains from different hosts in Iran. Parasitology Research, 101, 111–115. DOI: 10.1007/s00436-007-0461-7.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-c8da282e-fb1c-48dd-8ccf-a68840959cdd
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.