PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 35 | 07 |
Tytuł artykułu

Expression analysis of dehydrin multigene family across tolerant and susceptible barley (Hordeum vulgare L.) genotypes in response to terminal drought stress

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Dehydrins are one of the characteristic families of plant proteins that usually accumulate in response to drought. In the present study, gene expressions of dehydrin multigene family (13 genes) were examined in flag leaves of tolerant (Yousef) and susceptible (Moroco) barley varieties under terminal drought to characterize the involvement of dehydrins in the adaptive processes. The stomatal conductance, RWC, and Chl a, b contents had more reduction in Moroco than the Yousef which has more elevated osmotic adjustment. Drought stress increased significantly MDA and electrolyte leakage levels, but greater in Moroco, indicating a poor protection of cell and cytoplasmic membrane in this variety. Yousef variety had no reduction in grain yield under drought condition. Five genes (Dhn1, Dhn3, Dhn5, Dhn7 and Dhn9) were exclusively induced in Yousef under drought stress. In the stress condition, relative gene expression of Dhn3, Dhn9 had the direct correlations (P\0.05) with Chl a, b contents, osmotic adjustment, stomatal conductance, plant biomass and grain yield, and the negative correlations (P\0.05) with MDA and electrolyte leakage levels. The results supported the impending functional roles of dehydrin Kn and particularly YnSKn types in dehydration tolerance of barley during the reproductive stage.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
35
Numer
07
Opis fizyczny
p.2289-2297,fig.,ref.
Twórcy
autor
  • Department of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran
autor
  • Molecular Physiology Department, Agriculture Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
autor
  • Department of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran
  • Molecular Physiology Department, Agriculture Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
  • Department of Plant Breeding, Faculty of Agronomy Sciences, University of Tehran, Tehran, Iran
autor
  • Molecular Physiology Department, Agriculture Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
autor
  • Department of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran
Bibliografia
  • Abedini R, shahbazi M, shobbar ZS, pishkam R, ebrahimi A (2012) Expression analysis of dehydrins gene family in barley tolerant and sensitive cultivars and wild genotype under drought conditions. Iran J Plant Biol 11:39–46
  • Acar O, Türkan I, Özdemir F (2001) Superoxide dismutase and peroxidase activities in drought sensitive and resistant barley (Hordeum vulgare L.) varieties. Acta Physiol Plant 23(3): 351–356
  • Bannayan M, Sanjani S, Alizadeh A, Lotfabadi SS, Mohamadian A (2010) Association between climate indices, aridity index, and rainfed crop yield in northeast of Iran. Field Crops Res 118(2):105–114
  • Brini F, Hanin M, Lumbreras V, Amara I, Khoudi H, Hassairi A, Pages M, Masmoudi K (2007) Overexpression of wheat dehydrin DHN-5 enhances tolerance to salt and osmotic stress in Arabidopsis thaliana. Plant Cell Rep 26(11):2017–2026
  • Brini F, Saibi W, Amara I, Gargouri A, Masmoudi K, Hanin M (2010) Wheat Dehydrin DHN-5 Exerts a Heat-Protective Effect on. Beta-Glucosidase and Glucose Oxidase Activities. Biosci Biotechnol Biochem 74(5):1050–1054
  • Cattivelli L, Rizza F, Badeck F-W, Mazzucotelli E, Mastrangelo AM, Francia E, Mare C, Tondelli A, Stanca AM (2008) Drought tolerance improvement in crop plants: an integrated view from breeding to genomics. Field Crops Res 105(1):1–14
  • Ceccarelli S, Grando S, Baum M, Udupa SM (2004) Breeding for drought resistance in a changing climate. Challenges and strategies of dryland agriculture. CSSA Special Publication No. 32. Madison, WI
  • Choi D-W, Zhu B, Close T (1999) The barley (Hordeum vulgare L.) dehydrin multigene family: sequences, allele types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv Dicktoo. TAG. Theor Appl Genet 98(8):1234–1247
  • Close TJ (2006) Dehydrins: a commonalty in the response of plants to dehydration and low temperature. Physiol Plant 100(2):291–296
  • Du J-B, Yuan S, Chen Y-E, Sun X, Zhang Z-W, Xu F, Yuan M, Shang J, Lin H–H (2011) Comparative expression analysis of dehydrins between two barley varieties, wild barley and Tibetan hulless barley associated with different stress resistance. Acta Physiol Plant 33(2):567–574
  • Ederli L, Pasqualini S, Batini P, Antonielli M (1997) Photoinhibition and oxidative stress: effects on xanthophyll cycle, scavenger enzymes and abscisic acid content in tobacco plants. J Plant Physiol 151(4):422–428
  • Fischer R, Sayre K, Reynolds M (2005) Osmotic adjustment in wheat in relation to grain yield under water deficit environments. Agron J 97(4):1062–1071
  • Habibi G (2012) Exogenous salicylic acid alleviates oxidative damage of barley plants under drought stress. Acta Biol Szeged 56(1):57–63
  • Hara M, Fujinaga M, Kuboi T (2004) Radical scavenging activity and oxidative modification of citrus dehydrin. Plant Physiol Biochem 42(7):657–662
  • Hu L, Wang Z, Du H, Huang B (2010) Differential accumulation of dehydrins in response to water stress for hybrid and common Bermuda grass genotypes differing in drought tolerance. J Plant Physiol 167(2):103–109
  • Koag M-C, Wilkens S, Fenton RD, Resnik J, Vo E, Close TJ (2009) The K-segment of maize DHN1 mediates binding to anionic phospholipid vesicles and concomitant structural changes. Plant Physiol 150(3):1503–1514
  • Mahajan S, Tuteja N (2005) Cold, salinity and drought stresses: an overview. Arch Biochem Biophys 444(2):139–158
  • Nylander M, Svensson J, Palva ET, Welin BV (2001) Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana. Plant Mol Biol 45(3):263–279
  • Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9):2002–2007
  • Puhakainen T, Hess MW, Mäkelä P, Svensson J, Heino P, Palva ET (2004) Overexpression of multiple dehydrin genes enhances tolerance to freezing stress in Arabidopsis. Plant Mol Biol 54(5):743–753
  • Rodrıguez M, Canales E, Borrás-Hidalgo O (2005) Molecular aspects of abiotic stress in plants. Biotecnologı´a Aplicada 22(1):1–10
  • Rorat T, Szabala BM, Grygorowicz WJ, Wojtowicz B, Yin Z, Rey P (2006) Expression of SK 3-type dehydrin in transporting organs is associated with cold acclimation in Solanum species. Planta 224(1):205–221
  • Sun X, Yuan S, Lin H–H (2006) Salicylic acid decreases the levels of dehydrin-like proteins in Tibetan hulless barley leaves under water stress. Zeitschrift fur Naturforschung C J Biosci 61(3–4):245–250
  • Svensson J, Ismail AM, Tapio Palva E, Close TJ (2002) Dehydrins. In: Storey KB, Storey JB (eds) Cell and molecular responses to stress, vol 3. Elsevier Science, Amsterdam
  • Tommasini L, Svensson JT, Rodriguez EM, Wahid A, Malatrasi M, Kato K, Wanamaker S, Resnik J, Close TJ (2008) Dehydrin gene expression provides an indicator of low temperature and drought stress: transcriptome-based analysis of barley (Hordeum vulgare L.). Funct Integr Genomics 8(4):387–405
  • Wójcik-Jagła M, Rapacz M, Barcik W, Janowiak F (2012) Differential regulation of barley (Hordeum distichon) HVA1 and SRG6 transcript accumulation during the induction of soil and leaf water deficit. Acta Physiol Plant 34:2069–2078
Uwagi
rekord w opracowaniu
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-c65ea654-8d43-4a92-b95d-447771ab22c6
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.