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2013 | 35 | 07 |

Tytuł artykułu

Expression analysis of dehydrin multigene family across tolerant and susceptible barley (Hordeum vulgare L.) genotypes in response to terminal drought stress

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Dehydrins are one of the characteristic families of plant proteins that usually accumulate in response to drought. In the present study, gene expressions of dehydrin multigene family (13 genes) were examined in flag leaves of tolerant (Yousef) and susceptible (Moroco) barley varieties under terminal drought to characterize the involvement of dehydrins in the adaptive processes. The stomatal conductance, RWC, and Chl a, b contents had more reduction in Moroco than the Yousef which has more elevated osmotic adjustment. Drought stress increased significantly MDA and electrolyte leakage levels, but greater in Moroco, indicating a poor protection of cell and cytoplasmic membrane in this variety. Yousef variety had no reduction in grain yield under drought condition. Five genes (Dhn1, Dhn3, Dhn5, Dhn7 and Dhn9) were exclusively induced in Yousef under drought stress. In the stress condition, relative gene expression of Dhn3, Dhn9 had the direct correlations (P\0.05) with Chl a, b contents, osmotic adjustment, stomatal conductance, plant biomass and grain yield, and the negative correlations (P\0.05) with MDA and electrolyte leakage levels. The results supported the impending functional roles of dehydrin Kn and particularly YnSKn types in dehydration tolerance of barley during the reproductive stage.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

35

Numer

07

Opis fizyczny

p.2289-2297,fig.,ref.

Twórcy

autor
  • Department of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran
autor
  • Molecular Physiology Department, Agriculture Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
autor
  • Department of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran
  • Molecular Physiology Department, Agriculture Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
  • Department of Plant Breeding, Faculty of Agronomy Sciences, University of Tehran, Tehran, Iran
autor
  • Molecular Physiology Department, Agriculture Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
autor
  • Department of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran

Bibliografia

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  • Brini F, Saibi W, Amara I, Gargouri A, Masmoudi K, Hanin M (2010) Wheat Dehydrin DHN-5 Exerts a Heat-Protective Effect on. Beta-Glucosidase and Glucose Oxidase Activities. Biosci Biotechnol Biochem 74(5):1050–1054
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  • Choi D-W, Zhu B, Close T (1999) The barley (Hordeum vulgare L.) dehydrin multigene family: sequences, allele types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv Dicktoo. TAG. Theor Appl Genet 98(8):1234–1247
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  • Sun X, Yuan S, Lin H–H (2006) Salicylic acid decreases the levels of dehydrin-like proteins in Tibetan hulless barley leaves under water stress. Zeitschrift fur Naturforschung C J Biosci 61(3–4):245–250
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Bibliografia

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