Non- negative matrix factorization:a novel method to determine reference spectra of fluorescent dyesion a cellular environment

• Autorzy: Neher E.
• Języki publikacji: EN

Abstrakty

EN

All spectrally resolved fl uorescence-based methods depend critically on the availability of accurate reference spectra. Very often spectra in a cellular environment do not agree with those measured in a cuvette. Also, for a given measurement instrument-specifi c corrections have to be applied. We recently developed a method to extract reference spetra from standard fl uorescence image stacks, such as those provided by a Zeiss Meta microscope. This method also allows one to separate spectral components of several simultaneously present dyes on a pixel by pixel basis. It should be ideal to complement lux-FRET measurements, providing reference spectra in the presence of autofl uorescence.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.278

Twórcy

autor

Neher E.

• Department of MembraneBiophysics, Max-Plank-Institute for Biophysical Chemistry, Munchem, Germany