Influence of different oxygen and nitrogen mixtures on the survival of worker bees after anesthesia with carbon dioxide

• Autorzy: Madras-Majewska B. , Ochnio L. , Ochnio M. , Zajdel B. , Kaminski Z. , Ostaszewska T. , Kamaszewski M.
• Języki publikacji: EN

Abstrakty

EN

Bee anesthesia has long been widely used in scientific research and for practical purposes. Bee anesthesia is usually induced with carbon dioxide. It is required for such procedures as populating mating hives and cages, introducing queen bees and instrumental insemination. The oxygen to nitrogen ratio may play a very important role in the process of awakening. The aim of the experiment was to investigate the survival rate of worker bees after the application of different concentrations of oxygen and nitrogen in their recovery from anesthesia. The general purpose was to determine the optimal gas mixture for the fastest recovery and the longest survival of bees. The results of this study provide a basis for future experiments on queen bees aimed at accelerating the awakening from anesthesia with carbon dioxide during insemination as well as the initiation of laying eggs. The lowest bee mortality in the first days after awakening from anesthesia was observed in the group awoken in a mixture of 48.6% O₂ and 51.4% N₂, but the highest average survival rate was noted in the group awoken in 60% O₂ and 40% N₂.

Słowa kluczowe

EN

oxygen mixture; nitrogen mixture; survival; honey bee worker; bee; anesthesia; carbon dioxide

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2014
• Tom: 70
• Numer: 12
• Opis fizyczny: p.770-773,fig.,ref.

Twórcy

autor

Madras-Majewska B.

• Apiculture Division, Faculty of Animal Sciences Warsaw University of Life Sciences - SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland

autor

Ochnio L.

• Department of Informatics, Faculty of Applied Mathematics and Informatics, Warsaw University of Life Sciences - SGGW, Warsaw, Poland

autor

Ochnio M.

• Apiculture Division, Faculty of Animal Sciences Warsaw University of Life Sciences - SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland

autor

Zajdel B.

• Apiculture Division, Faculty of Animal Sciences Warsaw University of Life Sciences - SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland

autor

Kaminski Z.

• Apiculture Division, Faculty of Animal Sciences Warsaw University of Life Sciences - SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland

autor

Ostaszewska T.

• Department of Ichthybiology and Fisheries, Warsaw University of Life Sciences - SSGW, Warsaw, Poland

autor

Kamaszewski M.

• Department of Ichthybiology and Fisheries, Warsaw University of Life Sciences - SGGW, Warsaw, Poland

Bibliografia

• 1. Austin G. H.: Effect of carbon dioxide anesthesia on the bee behavior and expectation on life. Bee World 1955, 36, 45-47.
• 2. Bieńkowska M., Gerula D., Panasiuk B.: Instrumental insemination of honey bee queens during flight activity predisposition period. 1. onest of oviposition. J. Apic. Sci. 2011, 55(2), 53-66.
• 3. Bieńkowska M., Panasiuk B., Węgrzynowicz P., Gerula D.: Effect of different carbone dioxide gas concentrations used during the insemination of honey bee queens on starting oviposition. J. Apic. Sci. 2012, 56(1), 125-135.
• 4. Chuda-Mickiewicz B., Prabucki J., Samborski J., Perużyński G.: Wpływ dwutlenku węgla na dojrzewanie i wartość rozrodczą trutni. Pszczel. Zesz. Nauk. 2002, 34, 16.
• 5. Czekońska K.: Influence of carbon dioxide on Nosema apis infection of honeybees (Apis mellifera). J. Invertebr. Pathol. 2007, 95(2), 84-86.
• 6. Czekońska K.: The effect of different concentrations of carbon dioxide (CO₂) in a mixture with air or nitrogen upon the survival of the honey bee (Apis mellifera). J. Apicult. Res. 2009, 48(1), 67-71.
• 7. Ebadi E.: CO2 and low temperature narcosis on honey bees. Environ. Entomol. 1980, 9(1), 144-147.
• 8. Hoback W. W., Stanley D. W.: Insects in hypoxia. J. Insect. Physiol. 2001, 47, 533-542.
• 9. Konopacka Z.: Wpływ narkozy CO₂ i N₂O na wyniki sztucznego unasienniania matek pszczelich. Pszcz. Zesz. Nauk. 1991, 35, 3-17.
• 10. Madras-Majewska B., Jasiński Z.: Badanie wpływu różnych stężeń głównych składników powietrza tlenu i azotu na wybudzanie pszczół robotnic. XLIII Nauk. Konf. Pszczel. Puławy 2006, p. 79-80.
• 11. Madras-Majewska B., Kamiński Z., Zajdel B.: The survival and the awaking time of the worker bees after carbon dioxide anesthesia and gas treatment with different oxygen and nitrogen concentration, Ann. Warsaw Univ. of Life Sci. – SGGW, Anim. Sci. 2011, 49, 109-113.
• 12. Nerum K. van, Buelens H.: Hypoxia-controlled winter metabolism in honeybees (Apis mellifera). Comp. Biochem. Physiol. A 1997, 117, 445-455.
• 13. Ribbands C. R.: Changes of behavior of honey-bees following their recovery from anesthesia. J Exp. Biol. 1950, 27, 302-310.
• 14. Simpson J.: Effect of some anaesthetics on honeybees: nitrous, oxide, carbon dioxide, ammonium nitrate smoker fumes. Bee World 1954, 35(8), 149-155.
• 15. Skowronek W.: Wpływ dwutlenku węgla na funkcjonowanie corpora allata u robotnic pszczoły miodnej (Apis mellifera L.). Pszczel. Zesz. Nauk. 1982, 26, 3-13.
• 16. Skowronek W.: Wpływ dwutlenku węgla na pszczoły. Prace Instytutu Sadownictwa i Kwiaciarstwa. Seria D, nr 13. Monografie i Rozprawy. Puławy 1982.
• 17. Skowronek W., Jaycox E.: Wpływ CO₂ na pszczoły robotnice. Pszczel. Zesz. Nauk. 1974, 18, 107-117.
• 18. Weyel W., Wegener G.: Adenine nucleotide metabolism during anoxia and postanoxic recovery in insects. Experimentia 1996, 52, 474-480.
• 19. Wilde J., Sobiechowski K.: The effect of carbon dioxide treatment on mating of virgin honeybee queens. Pol. J. Natur. Sc. 2002, 12(3), 73-85.
• 20. Wilkaniec Z., Maciejewska M.: Przeżywalność robotnic pszczoły miodnej (Apis mellifera L.) usypianych różnymi sposobami w badaniach laboratoryjnych. Pszczel. Zesz. Nauk. 1995, 1, 7-10.