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2014 | 58 | 1 |

Tytuł artykułu

Characterisation of Staphylococcus aureus and Staphylococcus aureus – like strains isolated from table eggs

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was to provide a detailed phenotypic and genotypie charaeterisation of Staphylococcus aureus strains and the group of microorganisms with unusual biochemical patterns (called Staphylococcus aureus-Mke) isolated from table chicken eggs. All of the strains tested exhibited resistance to at least one of the 17 antibiotics tested, and 55.55% of isolates were found to be resistant to five or more of them. PCR used for detection of the methicillin resistance gene (mecA) confirmed the presence of a specific product of 533 bp in the case of two of the isolated S. aureus-hke strains. Analysis of the phylogenetic relationship between eight of S. aureus and ten S. aureus-like strains distinguished 18 macrorestriction profiles following digestion with Smal endonuclease, indicating that there were no identical strains with the same macrorestriction profile. However, the presence of methicillin-resistant strains indicates a serious risk to consumer health.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

58

Numer

1

Opis fizyczny

p.57-63,fig.,ref.

Twórcy

autor
  • Sub-Department of Preventive Veterinary and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland
autor
  • Sub-Department of Preventive Veterinary and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland
autor
  • Department of Microbiology, Institute of Biology, University of Bialystok, 15-950 Bialystok, Poland

Bibliografia

  • 1. Akineden O., Hassan A.A., Schneider E., Usleber E.: A coagulase- negative variant of Staphylococcus aureus from bovine mastitis milk. J Dairy Res 2011, 78, 38-42.
  • 2. Bannerman T.L.: Staphylococcus, Micrococcus, and other catalase positive cocci that grow aerobically. In: Manual of clinical microbiology, ASM Press, Washington D.C, 2003, pp. 264-282.
  • 3. Baumann-Popczyk A., Sadkowska-Todys M.: Foodbome infections and intoxications in Poland in 2010. Przegl Epidemiol 2012, 66, 241-248.
  • 4. Brakstad O.G., Maeland J.A., Tveten Y.: Multiplex polymerase chain reaction for detection of genes for Staphylococcus aureus thermonuclease and methicillin resistance and correlation with oxacillin resistance. APMIS 1993, 101, 681-688.
  • 5. Bystroń J., Podkowik M., Piasecki T., Wieliczko A., Molenda J., Bania J.: Genotypes and enterotoxin gene content of S. aureus isolates from poultry. Vet Microbiol 2010, 144, 498-501.
  • 6. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement 2011, 31, 68-78.
  • 7. Crass B.A., Bergdoll M.S.: Involvement of coagulase- negative staphylococci in toxic shock syndrome. J Clin Microbiol 1986, 23, 43-45.
  • 8. Cunha M.L.R.S., Peresi E., Calsolari R.A.O., Junior J.P.A.: Detection of enterotoxins genes in coagulase-negative staphylococci isolated from foods. Brazil J Microbiol 2006, 37, 70-74.
  • 9. Dinges M.M, Onvin P.M., Schlievert P.M.: Exotoxins of Staphylococcus aureus. Clin Microbiol Rev 2000, 13, 16-34.
  • 10. Goh S.H., Byrne S.K., Zhang J.L., Chow A.W.: Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphism. J Clin Microbiol 1992, 30, 1642 1645.
  • 11. Graham J.C., Murphy O.M., Stewart D., Keams A.M., Galloway A., Freeman R.: Comparison of PCR detection of mecA with methicillin and oxacillin disc susceptibility testing in coagulase-negative staphylococci. J Antimicrob Chemother 2000, 45,111-113.
  • 12. Hussain Z., Stoakes L., Lannigan R., Longo S., Nancekivell B.: Evaluation of screening and commercial methods for detection of methicillin resistance in coagulase-negative staphylococci. J Clin Microbiol 1998, 36, 273-274.
  • 13. Isigidi B.K., Mathieu A.M., Devriese L.A., Godard C., Van Hoof J.: Enterotoxin production in different Staphylococcus aureus biotypes isolated from food and meat plants. J Appl Bacteriol 1992, 72, 16-20.
  • 14. Kloss W.E., Bannerman T.L.: Update on clinical significance of coagulase-negative staphylococci. Clin Microbiol Rev 1994, 7, 117-140.
  • 15. Lotter L P., Genigeorgis C.A.: Isolation of coagulase-positive variants from coagulase-negative enterotoxigenic staphylococci. Zentralbl Bakteriol Orig A 1977, 239, 18-30.
  • 16. Luczak-Kadlubowska A., Krzyszton Russjan J., Hryniewicz W.: Characteristics of Staphylococcus aureus strains isolated in Poland in 1996 to 2004 that were deficient in species-specific proteins. J Clin Microbiol 2006, 44, 4018-4024.
  • 17. MacFaddin J.F.: Coagulase test. In: Biochemical test for identification of medical bacteria, Williams & Wilkins, Philadelphia, 2000, pp. 105-119.
  • 18. Malinowski E., Lassa H., Kłossowska A., Smulski S., Kaczmarowski M.: Atypical Staphylococcus aureus as an aetiological agent of mastitis in cows. Bull Vet Inst Pulawy 2009, 53, 383-387.
  • 19. Phonimdaeng P., O'Reilly M., Nowlan P., Bramley A.J., Foster T.J.: The coagulase of Staphylococcus aureus 8325-4 sequence analysis and virulence of site-specific coagulase- deficient mutants. Mol Microbiol 1990, 4, 393-404.
  • 20. Priest F.G., Austin B.: Modem Bacterial Taxonomy, Chapman & Hall, London, 1993, p. 25.
  • 21. Pyzik E., Marek A.: Characterization of bacteria of the genus Staphylococcus isolated from the eggs of Japanese quail (Coturnix coturnix japonica). Polish J Vet Sci 2012, 15, 767-772.
  • 22. Shimizu A., Berkhoff H.A., Kloss W.E., George C.G., Ballad D.N.: Genomic DNA fingerprinting, using pulsed-field gel electrophoresis of Staphylococcus intermedins isolated from dogs. Am J Vet Res 1996, 57, 1458-1462.
  • 23. Tenover F.C., Arbeit R.D., Goering R.V., Mickelsen P.A., Murray B.E., Persing D.H., Swaminathan B.: Interpreting chromosomal DNA restriction patterns produced by pulsed - field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995, 33, 2233-2239.
  • 24. Veras J.F., do Carmo L.S., Tong L.C., Shupp J.W., Cummings C., Dos Santos D.A., Cerqueira M M., Cantini A., Nicoli J.R., Jett M.: A study of the enterotoxygenicity of coagulase-negative and coagulase-positive staphylococcal isolates from food poisoning outbreaks in Minas Gerais, Brazil. Int J Infect Dis 2008, 12, 410- 415.
  • 25. Voss A., Doebbeling B.N.: The Worldwide prevalence of methicillin resistant Staphylococcus aureus. Int J Antimicrob Agents 1995, 5, 101-106.
  • 26. Wilkinson B.J., Maxwell S., Schaus S.M.: Classification and characteristics of coagulase negative, methicillin resistant staphylococci. J Clin Microbiol 1980, 12, 161-166.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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