Possibilities in identification of genomic species of Burkholderia cepacia complex by PCR and RFLP

• Autorzy: Navratilova L. , Chroma M. , Hanulik V. , Raclavsky V.
• Języki publikacji: EN

Abstrakty

EN

The strains belonging to Burkholderia cepacia complex are important opportunistic pathogens in immunocompromised patients and cause serious diseases. It is possible to obtain isolates from soil, water, plants and human samples. Taxonomy of this group is difficult. Burkholderia cepacia complex consists of seventeen genomic species and the genetic scheme is based on recA gene. Commonly, first five genomovars occurre in humans, mostly genomovars II and III, subdivision IIIA. Within this study we tested identification of first five genomovars by PCR with following melting analysis and RFLP. The experiments were targeted on eubacterial 16S rDNA and specific gene recA, which allowed identification of all five genomovars. RecA gene appeared as more suitable than 16S rDNA, which enabled direct identification of only genomovars II and V; genomovars I, III and IV were similar within 16S rDNA sequence.

Słowa kluczowe

EN

possibility; identification; genomic species; Burkholderia cepacia; polymerase chain reaction; RFLP method; bacteria; human pathogen; human disease

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2013
• Tom: 62
• Numer: 4
• Opis fizyczny: p.373-376,fig.,ref.

Twórcy

autor

Navratilova L.

• Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 775 15 Olomouc, Czech Republic

autor

Chroma M.

• Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 775 15 Olomouc, Czech Republic

autor

Hanulik V.

• Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 775 15 Olomouc, Czech Republic

autor

Raclavsky V.

• Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, 775 15 Olomouc, Czech Republic

Bibliografia

• Dřevínek P., H. Hrbáčová, O. Cinek, J. Bartošová, O. Nyč, A. Nemec and P. Pohunek. 2002. Direct PCR detection of Burkholderia cepacia complex and identification of its genomovars by using sputum as source of DNA. J. Clin. Microbiol. 40: 3485-3488.
• Frangollas D.D., E. Mahenthiralingam, S. Rae, J.M. Raboud, A.G.F. Davidson, R. Wittman and P.G. Wilcox. 1999. Burkholderia cepacia in cystic fibrosis: variable disease course. Am. J. Respir. Crit. Care. Med. 160: 1572-1577.
• Govan J.R.W. V.P. and Deretic. 1996. Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol. Rev. 60: 539-574.
• Henry D.A., M.E. Campbell, J.J. LiPuma J.J. and D.P. Speert. 1997. Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple nex selective medium. J. Clin. Microbiol. 35: 614-619.
• Henry D.A., E. Mahenthiralingam, P. Vandamme, T. Coenye and D.P. Speert. 2001. Phenotypic methods for determining genomovar statut of the Burkholderia cepacia complex. J. Clin. Microbiol. 39: 1073-1078.
• Isles A., I. Maduskey, M. Corey, R. Gold, C. Prober, P. Fleming and H. Levison. 1984. Pseudomonas cepacia infection in cystic fibrosis: an emerging problem. J. Pediatr. 104: 206-210. Jones A.M., M.E. Dodd, J.R.W. Govan, V. Barcus, C.J. Doherty, J. Morris and A.K. Webb. 2004. Burkholderia cenocepacia and Burkholderia multivorans: influence on survival in cystic fibrosis. Thorax 59: 948-951.
• Liu W, L. Li, M. Asaduzzaman and F. Zhu. 2012. Popular molecular markers in bacteria. Mol. Gen. Microbiol. Virusol. 27: 103-107.
• Mahenthiralingam E., A. Baldwin and P. Vandamme. 2002. Burkholderia cepacia complex infection in patients with cystic fibrosis. J. Med. Microbiol. 51: 533-538.
• Mahenthiralingam E., J. Bischof, S.K. Byrne, C. Radomski, J.E. Davies, Y. AV-Gay and P. Vandamme. 2000b. DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamensis, Burkholderia multivorans, Burkholderia stabilis and Burkholderia cepacia genomovar I and III. J. Clin. Microbiol. 38: 3165-3173.
• Mahenthiralingam E., T. Coenye, J.W Chung, D.P. Speert, J.R.W. Govan, P. Taylor and P. Vandamme. 2000a. Diagnostically and experimentally useful panel of strains from the Burkholderia cepacia complex. J. Clin. Microbiol. 38: 910-913.
• McDowell A., E. Mahenthiralingam, J.E. Moore, K.E. Dunbar, A.K. Webb, M.E. Dodd, S.L. Martin, B.C. Millar, C.J. Scott, M. Crowe and J.S. Elborn. 2001. PCR-based detection and identification of Burkholderia cepacia complex pathogens in sputum from cystic fibrosis patients. J. Clin. Microbiol. 39: 4247-4255.
• McMenamin J.D., T.M. Zaccone, T. Coenye, P. Vandamme and J.J. LiPuma. 2000. Misidentification of Burkholderia cepacia in US cystic fibrosis treatment centers - an analysis of 1,051 recent sputum isolates. Chest. 117: 1661-1665.
• Valnaere E., J.J. LiPuma, A. Baldwin, D. Henry, E. De Brandt, E. Mahenthiralingam, D. Speert, C. Dowson and P. Vandamme. 2008. Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. Int. J. Syst. Evol. Microbiol. 58: 1580-1590.
• Van Belkum A. 2003. High-throughput epidemiologic typing in clinical microbiology. Clin. Microbiol. Infect. 9: 86-100.
• Vonberg R.P., S. Häußler, P. Vandamme and I. Steinmetz. 2006. Identification of Burkholderia cepacia complex pathogens by rapid-cycle PCR with fluorescent hybridization probes. J. Med. Microbiol. 55: 721-727.
• Whitby P.W., K.B. Carter, K.L. Hatter, J.J. LiPuma and T.L. Stull. 2000. Identification of members of the Burkholderia cepacia complex by species-specific PCR. J. Clin. Microbiol. 38: 2962-2965.
• Zahariadis G., M.H. Levy and J.L. Burns. 2003. Cepacia-like syndrome caused by Burkholderia multivorans. Can. J. Infect. Dis. 14: 123-125.