Nowsze możliwości diagnozy ciąży u przeżuwaczy - ograniczenia, zalety, wykorzystanie

• Autorzy: Jaskowski J.M. , Herudzinska M. , Kmiecik J. , Kierbic A. , Jaskowski B.M. , Gehrke M.

Warianty tytułu

EN

New possibilities for pregnancy diagnosis in ruminants: Limitations, advantages, use

• Języki publikacji: PL

Abstrakty

EN

Early pregnancy diagnosis in ruminants, especially in high production herds, is one of the most important components of reproduction management. Reducing the time between insemination and the exclusion or confirmation of pregnancy is of major economic importance. That is why the traditional method of pregnancy examination by transrectal palpation is increasingly complemented by additional tests, such as ultrasonography. An alternative to these methods of pregnancy diagnosis are also simple field tests. The best known are tests for progesterone and estrone sulphate concentrations. Over the last few years, the measuring of pregnancyassociated glycoproteins (PAGs) has also been gaining popularity. In cattle, this method can be used as early as the 28th day of pregnancy, when the plasma concentration of PAGs exceeds 1ng/ml. Maximum values of pregnancy-associated glycoproteins are observed about day 240 after fertilization. After calving, their level quickly decreases. In sheep, the concentration of PAGs increases significantly in the 3rd and 4th weeks of gestation and reaches its maximum level around the 9th week. In goats, the concentration of PAGs differs between races, but its measurement can be quite a reliable indirect method of pregnancy diagnosis. The PAGs value in this species grows until about the 8th week after insemination and, depending on the breed, reaches a maximum value of 50 to 69 ng/ml. One limitation for the use of PAGs field tests may be false positives which occur after the death of the embryo. Similarly, an increased PAGs concentration can persists for up to about 70 days after calving. Moreover, the PAGs concentration is also affected by the health, age and milk production of the female. The advantages of field tests are the simplicity of performance, the ease of reading and interpreting the results and the short time needed to complete the entire procedure. Field tests based on the commonly used ELISA method are characterized by low invasiveness (blood, milk), combined with high sensitivity and specificity. Their rapid application under field conditions makes them an excellent extension of diagnostic capabilities, especially in large herds of cows.

Słowa kluczowe

PL

przezuwacze; bydlo; krowy; rozrod zwierzat; diagnostyka ciazy; diagnostyka wczesna; glikoproteiny ciazowe; bialko PAG; testy ciazowe dla bydla; testy PAG; dokladnosc; testy terenowe

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2018
• Tom: 74
• Numer: 06
• Opis fizyczny: s.349-355,tab.,bibliogr.

Twórcy

autor

Jaskowski J.M.

• Centrum Weterynarii, Uniwersytet im. Mikołaja Kopernika w Toruniu, ul.Gagarina 7, 87-100 Toruń

autor

Herudzinska M.

• Centrum Weterynarii, Uniwersytet im. Mikołaja Kopernika w Toruniu, ul.Gagarina 7, 87-100 Toruń

autor

Kmiecik J.

• Centrum Weterynarii, Uniwersytet im. Mikołaja Kopernika w Toruniu, ul.Gagarina 7, 87-100 Toruń

autor

Kierbic A.

• Centrum Weterynarii, Uniwersytet im. Mikołaja Kopernika w Toruniu, ul.Gagarina 7, 87-100 Toruń

autor

Jaskowski B.M.

• Katedra Rozrodu z Kliniką Zwierząt Gospodarskich, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy we Wrocławiu, pl.Grunwaldzki 49, 50-366 Wrocław

autor

Gehrke M.

• Centrum Weterynarii, Uniwersytet im. Mikołaja Kopernika w Toruniu, ul.Gagarina 7, 87-100 Toruń

Bibliografia

• Al-Hassan M. J., Al-Samawi K. A.: Seasonal variations in serum pregnancy associated glycoproteins during early pregnancy in Aardi goats in central Saudi Arabia. Asian Pac. J. Reprod. 2017, 226-230.
• Barbato O., Menchetti L., Sousa N. M., Malfatti A., Brecchia G., Canali C., Beckers J. F., Barile V. L.: Pregnancy-associated glycoproteins (PAGs) concentrations in water buffaloes (Bubalus bubalis) during gestation and the postpartum period. Theriogenology 2017, 97, 73-77.
• Carvalho P. D., Consentini C. C, Weaver S. R., Barletta R. V., Hernandez L. L., Fricke P. M.: Temporarily decreasing progesterone after timed artificial insemination decreased expression of interferon-tau stimulated gene 15 (ISG15) in blood leukocytes, serum PSPB concentrations, and embryo size in lactating Holstein cows. J. Dairy Sci. 2017, 100, 3233-3242.
• Commun L., Velek K., Barbry J. B., Pun S., Rice A., Mestek A., Egli C., Leterme S.: Detection of pregnancy-associated glycoproteins in milk and blood as a test for early pregnancy in dairy cows. J. Vet. Diagn. Invest. 2016, 28, 207-213.
• Dufour S., Durocher J., Dubuc J., Dendukuri N., Hassan S., Buczinski S.: Bayesian estimation of sensitivity and specificity of a milk pregnancy-associated glycoprotein-based ELISA and of transrectal ultrasonographic exam for diagnosis of pregnancy at 28-45 days following breeding in dairy cows. Prev. Vet. Med. 2017, 140, 122-133.
• El Amiri B., Sousa N. M., Alvarez Oxiley A., Hadarbach D., Beckers J. F.: Pregnancy-associated glycoprotein (PAG) concentration in plasma and milk samples for early pregnancy diagnosis in Lacaune dairy sheep. Res. Vet. Sci. 2015, 99, 30-36.
• Engelke J., Feldmann M., Gundling N., Gundelach Y., Egli C., Hoedemaker M., Piechotta M.: Pregnancy diagnosis by detection of pregnancy associated glycopoteins in milk: evaluation of a commercial available ELISA. Berl. Munch. Tierarztl. Wochenschr. 2015, 128, 402-408.
• Fricke P. M., Ricci A., Carvalho P. D., Mason C., Amundson M. C.: Milk vs. Blood – Which is Best for PAG Pregnancy Prediction? Western Dairy Management Conference, Reno, March 3-5, 2015.
• Gajewski Z., Melo de Sousa N., Konopka A. et al.: Determination of PAG in the milk and blood in cow’s in comparison to other examination methods for pregnancy diagnosis. Proc. XXV WBC, Budapest 2008, p. 92-97.
• Gajewski Z., Pawliński B., Wehrend A., Petrajkis-Golobow M., Siewruk K., Trela M., Dąbrowski M., Aniołek A.: Endokrynologia wczesnej ciąży u bydła, [w:] Gajewski Z., Wehrend A. (red.): Rozród bydła – wybrane zagadnienia. Apra-wetpress s.c., Warszawa 2014, s. 12-17.
• Gajewski Z., Petrajtis-Gołobów M., Melo de Sousa N., Beckers J. F., Pawliński B., Wehrend A.: Comparison of accuracy of pregnancy-associated glycoprotein (PAG) concentration in blood and milk for early pregnancy diagnosis in cows. Schweiz. Arch. Tierheilkd. 2014, 156, 585-590.
• Gandy B., Tucker W., Ryan P., Williams A., Tucker A., Moore A., Godfrey R., Willard S.: Evaluation of the early conception factor (ECF™) test for the detection of non pregnancy in dairy cattle. Theriogenology 2001, 56, 637-647.
• García-Ispierto I., Rosselló-Visa M. A., Serrano-Pérez B., Mur-Novales R., Sousa de N. M., Beckers J. F., López-Gatius F.: Plasma concentrations of pregnancy-associated glycoproteins I and II and progesterone on day 28 post-AI as markers of twin pregnancy in dairy cattle. Livestock Science 2016, 192, 44-47.
• Ghaffari Laleh V., Ghaffari Laleh R., Pirany N., Moghadaszadeh Ahrabi M.: Measurement of EPF for detection of cow pregnancy using rosette inhibition test. Theriogenology 2008, 70, 105-107.
• Giordano J. O., Guenther J. N., Lopes G. Jr., Fricke P. M.: Changes in serum pregnancy-associated glycoprotein, pregnancy specific protein B, and progesterone concentrations before and after induction of pregnancy loss in lactating dairy cows. J. Dairy Sci. 2012, 95, 683-697.
• González F., Cabrera F., Batista M., Rodrı́guez N., Álamo D., Sulon J., Beckers J. F., Gracia A.: A comparison of diagnosis of pregnancy in the goat via transrectal ultrasound scanning, progesterone, and pregnancy-associated glycoprotein assays. Theriogenology 2004, 62, 1108-1115.
• Hooshmandabbasi R., Zerbe H., Bauersachs S., de Sousa N. M., Boos A., Klisch K.: Pregnancy-associated glycoproteins in cows with retained fetal membranes. Theriogenology 2017, 105, 158-163.
• Jaśkowski J. M., Gehrke M., Herudzińska M., Jaśkowski B. M., Kulus J.: Szybki test ciążowy u krów – wstępne badania. Weterynaria w Terenie 2017 (w druku).
• Jaśkowski J. M., Herudzińska M., Kmiecik J., Jaśkowski B. M., Kierbić A., Kulus J.: Wstępne uwagi dotyczące wykorzystania testu terenowego do wykrywania wczesnej ciąży u krów. Lecznica Dużych Zwierząt 2017 (w druku).
• Karen A., Sousa N. M., Beckers J. F., Bajcsy Á. C., Tibold J., Mádl I., Szenci O.: Comparison of a commercial bovine pregnancy-associated glycoprotein ELISA test and a pregnancy-associated glycoprotein radiomimmuno assay test for early pregnancy diagnosis in dairy cattle. Anim. Reprod. Sci. 2015, 159, 131-137.
• Kornmatitsuk B., Dahl E., Ropstad E., Beckers J. E., Gustafsson H., Kindahl H.: Endocrine profiles, haematology and pregnancy out comes of late pregnant Holstein dairy heifers sired by bulls giving a high or low incidence of stillbirth. Acta. Vet. Scand. 2004, 45, 47-68.
• Kornmatitsuk B., Veronesi M. C., Madej A., Dahl E., Ropstad E., Beckers J. F., Forsberg M., Gustafsson H., Kindahl H.: Hormonal measurements in late pregnancy and parturition in dairy cows – possible tools to monitor foetal well being. Anim. Reprod. Sci. 2002, 72, 153-164.
• Lamglait B., Rambaud T.: Diagnosis of pregnancy in Barbary sheep (Ammotragus lerivia) using a bovine assay for pregnancy-associated glycoproteins. J. Zoo. Wildl. Med. 2017, 48, 525-528.
• Lawson B. C., Shahzad A. H., Dolecheck K. A., Martel E. L., Velek K. A., Ray D. L., Lawrence J. C., Silvia W. J.: A pregnancy detection assay using milk samples: evaluation and considerations. J. Dairy Sci. 2014, 97, 6316-6325.
• LeBlanc S. J.: Short communication: Field evaluation of a pregnancy confirmation test using milk samples in dairy cows. J. Dairy Sci. 2013, 96, 2345-2348.
• López-Gatius F., Garbayo J. M., Santolaria P., Yániz J., Ayad A., de Sousa N. M., Beckers J. F.: Milk production correlates negatively with plasma levels of pregnancy-associated glycoprotein (PAG) during the earlyfetal period in high producing dairy cows with live fetuses. Domest. Anim. Endocrinol. 2007, 32, 29-42.
• Max A.: Problemy z rozpoznawaniem ciąży u krów. Życie Wet. 2013, 88, 11.
• Mayo L. M., Moore S. G., Poock S. E., Silvia W. J., Lucy M. C.: Technical note: Validation of a chemical pregnancy test in dairy cows that uses whole blood, shortened incubation times and visual readout. J. Dairy Sci. 2016, 99, 7634-7641.
• Mercadante P. M., Ribeiro E. S., Risco C., Ealy A. D.: Associations between pregnancy-associated glycoproteins and pregnancy outcomes, milk yield, parity, and clinical diseases in high-producing dairy cows. J. Dairy Sci. 2016, 99, 3031-3040.
• Nation D. P., Malmo J., Davis G. M., Macmillan K. L.: Accuracy of bovine pregnancy detection using transrectal ultrasonography at 28 to 35 days after insemination. Aust. Vet. J. 2003, 81, 63-65.
• Pohler K. G., Pereira M. H., Lopes F. R., Lawrence J. C., Keisler D. H., Smith M. F., Vasconcelos J. L., Green J. A.: Circulating concentrations of bovine pregnancy-associated glycoproteins and late embryonic mortality in lactating dairy herds. J. Dairy Sci. 2016, 99, 1584-1594.
• Power S. G., Challis J. R.: Tissue-specific concentration changes of estrone and estradiol during spontaneous and ACTH-inducedparturition in sheep. Can. J. Physiol. Pharmacol. 1987, 65, 130-135.
• Ricci A., Carvalho P. D., Amundson M. C., Fourdraine R. H., Vincenti L., Fricke P. M.: Factors associated with pregnancy-associated glycoprotein (PAG) levels in plasma and milk of Holstein cows during early pregnancy and their effect on the accuracy of pregnancy diagnosis. J. Dairy Sci. 2015, 98, 2502-2514.
• Ricci A., Carvalho P. D., Amundson M. C., Fricke P. M.: Characterization of luteal dynamics in lactating Holstein cows for 32 days after synchronization of ovulation and timed artificial insemination. J. Dairy Sci. 2017 Oct 4. pii: S0022-0302(17)30911-6. doi: 10.3168/jds.2017-13293. [Epub ahead of print]
• Roberts J. N., Byrem T. M., Grooms D. L.: Application of an ELISA Milk Pregnancy Test in Beef Cows. Reprod. Domest. Anim. 2015, 50, 651-658.
• Roberts J. N., May K. J., Veiga-Lopez A.: Time-dependent changes in pregnancy-associated glycoproteins and progesterone in commercial crossbred sheep. Theriogenology 2017, 89, 271-279.
• Sasser R. G., Ruder C. A.: Detection of early pregnancy in domestic ruminants. J. Reprod. Fertil. Suppl. 1987, 34, 261-271.
• Serrano-Pérez B., Garcia-Ispierto I., Sousa N. M., Beckers J. F., Almería S., López-Gatius F.: Gamma Interferon Production and Plasma Concentrations of Pregnancy-Associated Glycoproteins 1 and 2 in Gestating Dairy Cows Naturally Infected With Neospora caninum. Reprod. Domest. Anim. 2014, 49, 275-280.
• Shahin M.: Pregnancy-associated glycoprotein (PAG) profiles in cows and goats and attempts to measure PAG in milk. Diss. Doktor., Georg-August-Universität, Göttingen 2013.
• Shahin M., Friedrich M., Gauly M., Beckers J. F., Holtz W.: Pregnancyassociated glycoprotein (PAG) pattern and pregnancy detection in Boer goats using an ELISA with different antisera. Small Rum. Res. 2013, 113, 141-144.
• Silva E., Sterry R. A., Kolb D., Mathialagan N., McGrath M. F., Ballam J. M., Fricke P. M.: Accuracy of a pregnancy-associated glycoprotein ELISA to determine pregnancy status of lactating dairy cows twenty-seven days after timed artificial insemination. J. Dairy Sci. 2007, 90, 4612-4622.
• Silva E., Sterry R. A., Kolb D., Mathialagan N., McGrath M. F., Ballam J. M., Fricke P. M.: Effect of interval to resynchronization of ovulation on fertility of lactating Holstein cows when using transrectal ultrasonography or a pregnancy-associated glycoprotein enzyme-linked immunosorbent assay to diagnose pregnancy status. J. Dairy Sci. 2009, 92, 3643-3650.
• Sinedino L. D., Lima F. S., Bisinotto R. S., Cerri R. L., Santos J. E.: Effect of early or late resynchronization based on different methods of pregnancy diagnosis on reproductive performance of dairy cows. J. Dairy Sci. 2014, 97, 4932-4941.
• Sousa N. M., Ayad A., Beckers J. F., Gajewski Z.: Pregnancy-associated glycoproteins (PAG) as pregnancy markers in the ruminants. J. Physiol. Pharmacol. 2006, 57, Suppl 8, 153-171.
• Stahmann F., Gauly M., Borstel von U. K., Holtz W.: Pre-Analytic Sources of Error with the Serum PAG-Pregnancy Test for Cows. J. Agr. Sci. 2015, 7, 10-40.
• Szenci O., Beckers J. F., Humblot P., Sulon J., Sasser G., Taverne M. A. M., Varga J., Baltusen R., Schekk G.: Comparison of ultrasonography, bovine pregnancy-specific protein B, and bovine pregnancy-associated glycoprotein 1 tests for pregnancy detection in dairy cows. Theriogenology 1998, 50, 77-88.
• Tandiya U., Nagar V., Yadav V. P., Ali I., Gupta M., Dangi S. S., Hyder I., Yadav B., Bhakat M., Chouhan V. S., Khan F. A., Maurya V. P., Sarkar M.: Temporal changes in pregnancy-associated glycoproteins across different stages of gestation in the Barbari goat. Anim. Reprod. Sci. 2013, 142, 141-148.
• Wynands E. M., LeBlanc S. J., Kelton D. F.: Short communication: The effect of storage conditions and storage duration on milk ELISA results for pregnancy diagnosis. J. Dairy Sci. 2017 Oct 4. pii: S0022-0302(17)30897-4. doi: 10.3168/jds.2017-12799. [Epub ahead of print]
• Zamfirescu S., Anghel A., Nadolu D., Dobrin N.: Plasmatic profiles of pregnancy-associated glycoprotein and progesterone levels during early pregnancy in carpathian goat. Annals RSCB 2011, 16, 50-53.

Identyfikatory

DOI

10.21521/mw.6016