Effect of L-cysteine in extender on post-thaw quality of Sahiwal bull semen

• Autorzy: Ansari M.S. , Rakha B. A. , Akhter S.
• Języki publikacji: EN

Abstrakty

EN

The study was conducted to evaluate the effect of addition of L-cysteine to tris-citric acid (TCA) extender on the post-thaw quality of Sahiwal bull semen. For this purpose, two consecutive ejaculates were collected from three Sahiwal bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50×106 motile spermatozoa ml-1) in TCA extender having L-cysteine either 0.0 (control) or 0.5, 1.0 or 2.0 mM. Diluted semen was cooled to 4°C for 2 h, equilibrated for 4 h at 4°C, filled in straws at 4°C, kept in liquid nitrogen vapours for 10 min and then stored in the liquid nitrogen. Thawing was performed after 24 h of storage, at 37°C for 30 s. and the sperm motility, viability, plasma membrane and acrosomal integrity were assessed. Higher (P<0.05) sperm motility, viability, plasma membrane and acrosomal integrity were observed using extenders containing 1.0 or 2.0 mM compared to those containing 0.5 or 0.0 mM L-cysteine. It is concluded that addition of L-cysteine (to reach 1.0-2.0 mM) in TCA extender improves the post-thaw quality of Sahiwal bull semen.

Słowa kluczowe

EN

L-cysteine; extender; post-thaw quality; Sahiwal; bull; semen; semen quality; cryopreservation; cysteine; bovine spermatozoon

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2011
• Tom: 29
• Numer: 3
• Opis fizyczny: p.197-203,fig.,ref.

Twórcy

autor

Ansari M.S.

• Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shab Arid Agriculture University of Ravalpindi, Ravalpindi-46300, Pakistan

autor

Rakha B. A.

autor

Akhter S.

Bibliografia

• ALVAREZ J.G., STOREY B.T., 1983 – Role of superoxide dismutase in protecting rabbit spermatozoa from O2 toxicity due to lipid peroxidation. Biology of Reproduction 28, 183-197.
• ANDRABI S.M.H., SIDDIQUE M., ULLAH N., KHAN L.A., 2006 – Effect of reducing sperm number per insemination dose on fertility of cryopreserved buffalo bull semen. Pakistan Veterinary Journal 26, 17-19.
• ANSARI M.S., RAKHA B.A., ANDRABI S.M.H., ULLAH N., AKHTER S., 2010 – Cryopreservation of Sahiwal bull epididymal spermatozoa. Pakistan Journal of Zoology 42, 741-743.
• ANSARI M.S., RAKHA B.A., ULLAH N., ANDRABI S.M.H., KHALID M., AKHTER S., 2011 – Effect of L-cysteine in tris-citric egg yolk extender on post thaw quality of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa. Pakistan Journal of Zoology 43 (in press).
• ATESSAHIN A., BUCAK M.N., TUNCER P.B., KIZIL M., 2008 – Effects of antioxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze–thawing process. Small Ruminant Research 77, 38-44.
• BAILEY JL., BILODEAU J.F., CORMIER N., 2000 – Semen cryopreservation in domestic animals:A damaging and capacitating phenomena. Journal of Andrology 22, 508-515.
• BAUMBER J., BALL B.A., LINFOR J.J., 2005 – Assessment of the cryopreservation of equine spermatozoa in the presence of enzyme scavengers and antioxidants. American Journal of Veterinary Research 66, 772-779.
• BILODEAU J.F., BLANCHETTE S., GAGNON C., SIRAD M.A., 2000 – Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Molecular Reproduction and Development 55, 282-288.
• BUCAK M.N., ATESSAHIN A., YUCE A., 2008 – Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze-thawing process. Small Ruminant Research 75, 128-134.
• COTRAN R.S., KUMAR V., ROBINS S.L., 1989 – Pathologic basis of diseases, 4th edition, WB Saunders Co. Philadelphia, pp. 9-16.
• GADEA J., SELLES E., MARCO M.A., COPY P., MATAS C., ROMAR R., RUIZ S., 2004 –Decrease in glutathione content in boar sperm cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology 62, 690-701.
• GARCIA O., MAHMOOD K., HEMME T., 2003 – A review of milk production in Pakistan with particular emphasis on small scale producers. International Farm Comparison Network (IFCN).
• JEYENDRAN R.S., VAN DER VEN H.H., PEREZ-PELAEZ M., CRABO B.G., ZANEVELD L.J.D.,1984 – Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility 70, 219-228.
• KANKOFER M., KOLM G., AURICH J., 2005 – Activity of glutathione peroxidase, superoxide dismutase and catalase and lipid peroxidation intensity in stallion semen during storage at 5oC.Theriogenology 63, 1354-1365.
• KOVACS A., FOOTE R.H., 1992 – Viability and acrosome staining of bull, boar and rabbit spermatozoa. Biotechnic and Histochemistry 67, 119-124.
• LENZI A., GANDINI L., LOMABARDO F., PICARDO M., MARESCA V., PANFILI E., 2002 –Polyunsaturated fatty acids of germ cell membranes, glutathione and glutathione-dependant enzyme-PHGPx from basic to clinic. Contraception 65, 301-304.
• MEISTER A., ANDERSSSON M.E., 1983 – Glutathione. Annual Review of Biochemistry 52, 711-760.
• NICHI M., BOLS P.E.G., ZUGE R. M., BARNABE V.H., GOOVAERTS I.G.F., BARNABE R.C.,CORTADA C.N.M., 2006 – Seasonal variation in semen quality in Bos indicus and Bos taurus bulls raised under tropical conditions. Theriogenology 66, 822-828.
• OZKAN S.S., BUCAK M.N., TUNCER P.B., ULUTAŞ P.A., BILGEN A., 2009 – The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation. Cryobiology 58, 134-138.
• SAACKE R.G., WHITE J.M., 1972 – Semen quality tests and their relationship to fertility. Proceedings of the 4th Technical Conference on Artificial Insemination and Reproduction, N.A.A.B., 22-27.
• TARTAGLIONE CM., RITTA M.N., 2004 – Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen. Theriogenology 62, 1245-1252.
• THOMAS C.A., GARNER D.L., DE-JARNETTE J.M., MARSHALL C.E., 1997 – Fluorometric assessment of acrosomal integrity and viability in cryopreserved bovine spermatozoa. Biology of Reproduction 45, 880-887.
• UYSAL O., BUCAK M.N., 2007 – Effect of oxidized serum albumin, cysteine and lycopene on the quality of frozen thawed ram semen. Acta Veterinaria 76, 383-390.