Enzymatic profile, adhesive and invasive properties of Candida albicans under the influence of selected plant essential oils

• Autorzy: Budzynska A. , Saowska B. , Wieckowska-Szakiel M. , Rozalska B.
• Języki publikacji: EN

Abstrakty

EN

 The influence of essential oils (EOs) used at sublethal level, on the presence and intensity of Candida albicans virulence factors was evaluated. Minimal inhibitory concentrations (MICs) of Lemon balm, Citronella, Geranium and Clove oils were established as 0.097% (v/v). Using the agar plates with substrates for proteases, phospholipases and hemolysins it was shown that C. albicans ATCC 10231 and C. albicans ATCC 90028 strains differed in the type and amount of enzymes produced. No significant difference in their total amount could be detected after pretreatment for 24 h with EOs at ½ MIC. However, the short-term (1 h) acting oils at MIC caused a statistically significant reduction in this activity. In the API ZYM test it was demonstrated that both strains exhibited activity of the same 9 out of 19 enzyme types and that EOs caused a significant decrease in the release of some of them. In the presence of subMIC of EOs, or when the fungus had previously been exposed to the MIC of oil, germ tubes formation was significantly and irreversibly reduced. Such C. albicans spotted on the Spider agar containing EOs at subMICs were unable to penetrate the agar. A significant decrease in the C. albicans adhesion to the fibroblast monolayer with respect to controls was also demonstrated when yeasts had been exposed to EOs at MIC (1 h) in liquid medium. Thus, it has been shown that tested oils, used even at subMIC, exhibit significant activity reducing the presence/quantity of important C. albicans virulence factors.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2014
• Tom: 61
• Numer: 1
• Opis fizyczny: p.115-121,fig.,ref.

Twórcy

autor

Budzynska A.

• Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Lodz, Poland

autor

Saowska B.

• Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Lodz, Poland

autor

Wieckowska-Szakiel M.

• Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Lodz, Poland

autor

Rozalska B.

• Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Lodz, Poland

Bibliografia

• Agarwal V, Lal P, Pruthi V (2008) Prevention of Candida albicans biofilm by plant oils. Mycopathologia 165: 13-19. 
• Alviano DS, Alviano CS (2009) Plant extracts: search for new alternatives to treat microbial diseases. Curr Pharm Biotechnol 10: 106-121. 
• Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils - a review. Food Chem Toxicol 46: 446-475. 
• Biswas S, Dijck PV, Datta A (2007) Environmental sensing and signal transduction pathways regulating morphopathogenic determinants of Candida albicans. Microbiol Mol Biol Rev 71: 348-376. 
• Budzyńska A, Sadowska B, Lipowczan G, Maciąg A, Kalemba D, Różalska B (2013) Activity of selected essential oils against Candida spp. strains. Evaluation of new aspects of their specific pharmacological properties, with special reference to Lemon Balm. Adv Microbiol 3: 317-325.
• Carrasco H, Raimondi M, Svetaz L, Di Liberto M, Rodriguez MV, Espinoza L, Madrid A, Zacchino S (2012) Antifungal activity of eugenol analogues. Influence of different substituents and studies on mechanism of action. Molecules 17: 1002-1024. 
• Cleary IA, Saville SP (2010) An analysis of the impact of NRG1 overexpression on the Candida albicans response to specific environmental stimuli. Mycopathologia 170: 1-10. 
• Deorukhkar S, Saini S (2013) Evaluation of phospholipase activity in biofilm forming Candida species isolated from intensive care unit patients. Br Microbiol Res J 3: 440-447.
• Edris AE (2007) Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytother Res 21: 308-323. 
• Gácser A, Stehr F, Kröger C, Kredics L, Schäfer W, Nosanchuk JD (2007) Lipase 8 affects the pathogenesis of Candida albicans. Infect Immun 75: 4710-4718. 
• Hammer KA, Carson CF, Riley TV. (2000) Melaleuca alternifolia (tea tree) oil inhibits germ tube formation by Candida albicans. Med Mycol 38: 355-362. 
• Hiller E, Zavrel M, Hauser N, Sohn K, Burger-Kentischer A, Lemuth K, Rupp S (2011) Adaptation, adhesion and invasion during interaction of Candida albicans with the host - focus on the function of cell wall proteins. Int J Med Microbiol 301: 384-389. 
• Kabir MA, Ahmad Z (2013) Candida infections and their prevention. ISRN Preventive Medicine 2013:  http://dx.doi.org/10.5402/2013/763628.
• Khan MSA, Ahmad I (2012) In vitro influence of certain essentials oils on germ tube formation, cell surface hydrophobicity, and production of proteinase and hemolysin in Candida albicans. J Nat Pharm 3: 110-117.
• Liu Y, Filler SG (2011) Candida albicans Als3, a multifunctional adhesin and invasin. Eukaryot Cell 10: 168-173.  
• Martin KW, Ernst E (2004) Herbal medicines for treatment of fungal infections: a systematic review of controlled clinical trials. Mycoses 47: 87-92. 
• Meiller TF, Hube B, Schild L, Shirtliff ME, Scheper MA, Winkler R, Ton A, Jabra-Rizk MA (2009) A novel immune evasion strategy of Candida albicans. Proteolytic cleavage of a salivary antimicrobial peptide. PLoS One 4: e5039. 
• Messer SA, Jones RN, Fritsche TR (2006) International Surveillance of Candida spp. and Aspergillus spp. Report from the SENTRY Antimicrobial Surveillance Program (2003) J Clin Microbiol 44: 1782-1787. 
• Naglik J, Albrecht A, Bader O, Hube B (2004) Candida albicans proteinases and host/pathogen interactions. Cell Microbiol 6: 915-926. 
• Naglik JR, Moyes DL, Wächtler B, Hube B (2011) Candida albicans interactions with epithelial cells and mucosal immunity. Microbes Infect 13: 963-976. 
• Negri MF, Faria MG, Guilhermetti E, Alves AA, Paula CR, Svidzinski TIE (2010) Hemolytic activity and production of germ tubes related to pathogenic potential of clinical isolates of Candida albicans. Rev Ciênc Farm Básica Apl 31: 89-93.
• Papon N, Courdavault V, Clastre M, Bennett RJ (2013) Emerging and emerged pathogenic Candida species: beyond the Candida albicans paradigm. PLoS Pathog 9: e1003550.  
• Rajeshkumar R, Sundararaman M (2012) Emergence of Candida spp. and exploration of natural bioactive molecules for anticandidal therapy - status quo. Mycoses 55: e60-e73. 
• Reichling J, Schnitzler P, Suschke U, Saller R (2009) Essential oils of aromatic plants with antibacterial, antifungal, antiviral and cytotoxic properties - an overview. Forsch Komplementmed 16: 79-90. 
• Silva F, Ferreira S, Duarte A, Mendonça DI, Domingues FC (2011) Antifungal activity of Coriandrum sativum essential oil, its mode of action against Candida species and potential synergism with amphotericin B. Phytomedicine 19: 42-47. 
• Sorgo AG, Heilmann CJ, Brul S, de Koster CG, Klis FM (2013) Beyond the wall: Candida albicans secret(e)s to survive. FEMS Microbiol Lett 338: 10-17. 
• Thompson DS, Carlisle PL, Kadosh D (2011) Coevolution of morphology and virulence in Candida species. Eukaryot Cell 10: 1173-1182. 
• Tlamçani Z, Er-rami M (2013) Fungal opportunist infection: common and emerging fungi in immunocompromised patients. J Immunol Tech Infect Dis 2: doi: 10.4172/2329-9541.1000110
• Wächtler B, Citiulo F, Jablonowski N, Förster S, Dalle F, Schaller M, Wilson D, Hube B (2012) Candida albicans-epithelial interactions: dissecting the roles of active penetration, induced endocytosis and host factors on the infection process. PLoS One 7: e36952. 
• Warnke PH, Sherry E, Russo PA, Açil Y, Wiltfang J, Sivananthan S, Sprengel M, Roldàn JC, Schubert S, Bredee JP, Springer IN (2006) Antibacterial essential oils in malodorous cancer patients: Clinical observations in 30 patients. Phytomedicine 13: 463-467.