Light affects in vitro organogenesis of Linum usitatissimum L. and its cyanogenic potential

• Autorzy: Siegien I , Adamczuk A. , Wroblewska K.
• Języki publikacji: EN

Abstrakty

EN

The relationships between organogenesis of oil flax (Linum usitatissimum L., cv. ‘Szafir’) in vitro, cyanogenic potential (HCN-p) of these tissues and light were investigated. Shoot multiplication obtained on Murashige and Skoog medium containing 0.05 mg L-1 2,4-dichlorophenoxyacetic acid and 1 mg L-1 6-benzyladenine (BA), was about twice higher in light-grown cultures than those in darkness. Light-grown explants showed also higher rate of roots regeneration (in medium containing 1 mg L-1 a-naphtaleneacetic acid and 0.05 mg L-1 BA) than darkgrown ones. The cyanogenic potential (expressed both as linamarin and lotaustralin content and linamarase activity) of flax cultured in vitro was tissue-specific and generally was higher under light conditions than in darkness. The highest concentration of linamarin and lotaustralin was detected in light-regenerated shoots, and its amount was twice as high as in roots, and about threefold higher than in callus tissue. The activities of linamarase and β-cyanoalanine synthase in light-regenerated organs were also higher than those in darkness. Thus, higher frequency of regeneration of light-grown cultures than dark-grown ones seems to be correlated with higher HCN-p of these tissues. We suggest that free HCN, released from cyanoglucosides potentially at higher level under light conditions, may be involved in some organogenetic processes which improve regeneration efficiency.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 03
• Opis fizyczny: p.781-789,fig.,ref.

Twórcy

autor

Siegien I

autor

Adamczuk A.

autor

Wroblewska K.

Bibliografia

• Abbadi A, Domergue F, Bauger J, Napier JA, Welti R, Zahringer U, Cirpus P, Heinz E (2004) Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oilseeds: constrains on their accumulation. Plant Cell 16:2734–2748
• Alam EA (2010) Phytochemical screening on calli of Fagonia indica and Fagonia bruguieti Dc. N Y Sci J 3:158–164
• Bertazza G, Baraldi R, Predieri S (1995) Light effects on in vitro rooting of pear cultivars of different rhizogenic ability. Plant Cell Tissue Organ Cult 41:139–143
• Bertram L, Lercari B (2000) Phytochrome A and phytochrome B1 control the acquisition of competence for shoot regeneration in tomato hypocotyls. Plant Cell Rep 19:604–609
• Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
• Burns AE, Gleadow RM, Woodrow IE (2002) Light alters the allocation of nitrogen to cyanogenic glycosides in Eucalyptus cladocalyx. Oecologia 133:288–294
• Caillot S, Rosiau E, Laplace C, Thomasset B (2009) Influence of light intensity and selection on regeneration time of transgenic flax plants. Plant Cell Rep 28:359–371
• Collinge DB, Hughes MA (1982) Developmental and physiological studies on the cyanogenic glucosides of white clover, Trifolium repens L. J Exp Bot 33:154–161
• Cutler AJ, Sternberg M, Conn EE (1985) Properties of microsomal enzyme system from Linum usitatissimum (linen flax) which oxidizes valine to acetone cyanohydrin and isoleucine to 2-methylbutanone cyanohydrin. Arch Biochem Biophys 238:272–279
• Dedicˇova´ B, Hricova´ A, S ˇ amaj J, Obert B, Boba´k M, Pret’ova´ A (2000) Shoot and embryo like structures regenerated from cultured flax (Linum usitatissimum L.) hypocotyl segments. J Plant Physiol 157:327–334
• Elias M, Sudhakaran PR, Nambisan B (1997) Purification and characterisation of b-cyanoalanine synthase from cassava tissues. Phytochemistry 46:469–472
• Gaisser S, Heide L (1996) Inhibition and regulation of shikonin biosynthesis in suspension cultures of Lithospermum. Phytochemistry 41:1065–1072
• Garcia R, Cidade D, Castellar A, Lips A, Magioli A, Callado C, Mansur E (2007) In vitro morphogenesis patterns from shoot apices of sugar cane are determined by light and type of growth regulator. Plant Cell Tissue Organ Cult 90:181–190
• Gleadow RM, Woodrow IE (2002) Constrains on effectiveness of cyanogenic glycosides in herbivore defense. J Chem Ecol 28:1301–1313
• Gniazdowska A, Krasuska U, Bogatek R (2010) Dormancy removal in apple embryos by nitric oxide or cyanide involves modifications in ethylene biosynthetic pathway. Planta 232:1397–1407
• Gomes da Cunha AC, Ferreira A (1996) Somatic embryogenesis, organogenesis and callus growth kinetics of flax. Plant Cell Tissue Organ Cult 47:1–8
• Hahlbrock K, Conn EE (1971) Evidence for the formation of linamarin and lotaustralin in flax seedlings by the same glucosyltransferase. Phytochemistry 10:1019–1023
• Hassanein A, Dorion N (2005) Efficient plant regeneration system from leaf discs of zonal (Pelargonium x hortorum) and two scented (P. capitatum and P. graveolens) geraniums. Plant Cell Tissue Organ Cult 83:231–240
• Ho¨sel W, Berlin J, Hanzlik TN, Conn EE (1985) In vitro biosynthesis of 1-(40-hydroxyphenyl)-2-nitroethane and production of cyanogenic compounds in osmotically stressed cell suspension cultures of Escholtzia californica Cham. Planta 166:176–181
• Istock U, Lieberei R, Harms H (1990) Pattern of enzymes involved in cyanogenesis and HCN metabolism in cell cultures of Phaseolus lunatus L. varieties. Plant Cell Tissue Organ Cult 22:105–112
• Ja¨ger AK, McAlister BG, van Staden J (1995) Cyanogenic glycosides in leaves and callus cultures of Schlechterina mitostemmatoides. S Afr Tydskr Plant 61:274–275
• Joseph T, Yeoh H-H, Loh C-S (1999) Cyanogenesis in somatic embryos and plantlets of cassava (Manihot esculenta Crantz). J Sci Food Agric 79:1071–1074
• Kadkade P, Seibert M (1977) Phytochrome-regulated organogenesis in lettuce tissue culture. Nature 270:49–50
• Kaira C, Babbar SB (2010) Nitric oxide promotes in vitro organogenesis in Linum usitatissimum L. Plant Cell Tissue Organ Cult 103:353–359
• Kakes P (1990) Properties and functions of the cyanogenic system in higher plants. Euphytica 48:25–43
• Kakes P (1991) A rapid and sensitive method to detect cyanogenesis using microtiterplates. Biochem Syst Ecol 19:519–522
• Kojima M, Poulton JE, Thayer SS, Conn EE (1979) Tissue distributions of dhurrin and of enzymes involved in its metabolism in leaves of Sorghum bicolor. Plant Physiol 63:1022–1028
• Lieberei R, Nahrstedt A, Selmar D, Gasparotto L (1986) The occurrence of lotaustralin in the genus Hevea and changes of HCN-potential in developing organs of Hevea brasiliensis. Phytochemistry 25:1573–1578
• Liu JR, Sink KC, Dennis FG (1983) Plant regeneration from apple seedling explants and callus cultures. Plant Cell Tissue Organ Cult 2:293–304
• Liu C, Guo C, Wang Y, Ouyang F (2002) Effect of light irradiation on hairy root growth and artemisinin biosynthesis of Artemisia annua L. Process Biochem 38:581–585
• Millam S, Obert B, Pret’ova´ A (2005) Plant cell and biotechnology studies in Linum usitatissimum—a review. Plant Cell Tissue Organ Cult 82:93–103
• Møller BL (2010) Functional diversifications of cyanogenic glucosides. Curr Opin Plant Biol 13:338–347
• Mundhara R, Rashid A (2001) Regeneration of shoot-buds on hypocotyl of Linum seedlings: a stress-related response. Plant Sci 161:19–25
• Mundhara R, Rashid A (2002) Stimulation of shoot-bud regeneration on hypocotyl of Linum seedlings, on a transient withdrawal of calcium: effect of calcium, cytokinin and thidiazuron. Plant Sci 162:211–214
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
• Nahrstedt A, Kant J-D, Ho¨sel W (1984) Aspect on the biosynthesis of the cyanogenic glucoside triglochinin in Triglochin maritima. Planta Med 36:394–397
• Niedźwiedź-Siegień I (1998) Cyanogenic glucosides in Linum usitatissimum L. Phytochemistry 49:59–63
• Niedźwiedź-Siegień I, Giersimiuk A (2001) Environmental factors affecting the cyanogenic potential of flax seedlings. Acta Physiol Plant 23:383–390
• Obert B, Benson EE, Millam S, Pret’ova´ A, Bremner DH (2005) Moderation of morphogenetic and oxidative stress responses in flax in vitro cultures by hydroxynonenal and desferrioxamine. J Plant Physiol 162:537–547
• Oracz K, El-Maarouf-Bouteau H, Kranner I, Bogatek R, Corbineau F, Bailly C (2009) The mechanisms involved in seed dormancy alleviation by hydrogen cyanide unravel the role of reactive oxygen species as key factors of cellular signaling during germination. Plant Physiol 150:494–505
• Pa´dua VLM, Fernandes LD, Oliveira DE, Mansur E (1998) Effects of auxin and light treatments of donor plants on shoot production from Indica-type rice (Oryza sativa L.). In vitro Cell Dev Biol Plant 34:285–288
• Rutkowska-Krause I, Mankowska G, Łukaszeiwcz M, Szopa J (2003) Regeneration of flax (Linum usitatissimum L.) plants from anther culture and somatic tissue with increased resistance to Fusarium oxysporum. Plant Cell Rep 22:110–116
• Sanchez-Perez R, Jorgensen K, Motawia MS, Dicenta F, Møller BL (2009) Tissue and cellular localization of individual betaglycosidases using a substrate specific sugar reducing assay. Plant J 60:894–906
• Selmar D, Lieberei R, Biehl B (1988) Mobilisation and utilisation of cyanogenic glycosides. Plant Physiol 86:711–716
• Siegień I (2009) Cyanogenic compounds. In: Narwal SS, Bogatek R, Zagdan´ska BM, Sampietro DA, Vattuone MA (eds) Plant biochemistry. Studium Press LLC, Houston, pp 337–366
• Siegień I, Bogatek R (2006) Cyanide action in plants—from toxic to regulatory. Acta Physiol Plant 28:483–497
• Szechyńska-Hebda M, Skrzypek E, Dabrowska G, Biesaga-Kościelak J, Filek M, Wędzony M (2007) The role of oxidative stress induced by growth regulators in the regeneration process of wheat. Acta Physiol Plant 29:327–337
• Tyburski J, Tretyn A (2004) The role of light and polar auxin transport in root regeneration from hypocotyls of tomato seedling cuttings. Plant Growth Regul 42:39–48
• Verdus M-C, Thellier M, Ripol C (1997) Storage of environmental signals in flax. Their morphogenic effect as enabled by a transient depletion of calcium. Plant J 12:1399–1410
• Vetter J (2000) Plant cyanogenic glycosides. Toxicon 38:11–36
• Vickery PJ, Wheeler JL, Mulcahy C (1987) Factors affecting the hydrogen cyanide potential of white clover (Trifolium repens L.). Aust J Agric Res 38:1053–1059
• Wurtele ES, Nikolau BJ, Conn EE (1984) Tissue distribution of betacyanoalanine synthase in leaves. Plant Physiol 75:979–982
• Yeoh HH (1989) Kinetic properties of beta-glucosidase from cassava. Phytochemistry 28:721–724
• Yildiz M, O¨ zgen M (2004) The effect of a submersion pretreatment on in vitro explant growth and shoot regeneration from hypocotyls of flax (Linum usitatissimum). Plant Cell Tissue Organ Cult 77:111–115
• Zagrobelny M, Bak S, Rasmussen AV, Jørgensen B, Naumann CM, Møller BL (2004) Cyanogenic glucosides and plant-insect interactions. Phytochemistry 65:293–306
• Zhao P, Wang W, Feng F-S, Wu F, Yang Z-Q, Wang W-J (2007) High-frequency shoot regeneration through transverse thin cell layer culture in Dendrobium Candidum Wall Ex Lindl. Plant Cell Tissue Organ Cult 90:131–139

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