Genome-wide identification of Ziziphus jujuba TCP transcription factors and their expression in response to infection with jujube witches’ broom phytoplasma

• Autorzy: Chen P. , Li J. , Ye X. , Tan B. , Zheng X. , Cheng J. , Wang W. , Wang H. , Gu L. , Feng J.
• Języki publikacji: EN

Abstrakty

EN

Phytoplasmas are plant pathogenic bacteria that inhabit the phloem sieve elements in infected plants and lack a cell wall. Infection of the woody perennial Ziziphus jujuba with phytoplasma causes jujube witches’ broom (JWB), a disease that causes heavy crop losses. The TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factors (TFs) are plant-specific regulators of plant morphogenesis, development, and defense after phytoplasma infection. While the TCP gene family has been studied in many plants, there is no report on TCPs in jujube. In this study, 21 ZjTCP TFs were identified and bioinformatically analyzed based on the genome of the Dongzao cultivar. Another 2 ZjTCPs were found in the genome of the Junzao cultivar. Physicochemical properties of the ZjTCP proteins were quite varied, indicating possible versatility of functions. ZjTCP proteins ranged from 172 to 590 amino acids (aa), had isoelectric points (pIs) between 5.53 (ZjTCP1) and 9.81 (ZjTCP11), and molecular weights ranging from 19,279.98 (ZjTCP11) to 61,784.17 kDa (ZjTCP19). Interaction network mapping predicted several hubs, such as ZjTCP6, ZjTCP7, ZjTCP8, ZjTCP15, ZjTCP16, and ZjTCP19, among which ZjTCP6 and ZjTCP16 were predicted to function in plant defense and morphogenesis. Spatiotemporal expression analysis of the ZjTCPs showed that ten of the ZjTCP genes were detected after infection with ‘Candidatus Phytoplasma ziziphi’. ZjTCP6, ZjTCP7, ZjTCP16, and ZjTCP19 were up-regulated after phytoplasma infection. ZjTCP16 showed the most significant increase in transcript levels, after the emergence of disease symptoms. ZjTCP12, ZjTCP15, and ZjTCP18 were down-regulated after phytoplasma infection. We concluded that ZjTCP6 and ZjTCP16 were most likely regulatory factors with roles during the plant response to jujube witches’ broom.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2019
• Tom: 41
• Numer: 06
• Opis fizyczny: Article 86 [11p.], fig.,ref.

Twórcy

autor

Chen P.

• College of Forestry, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China

autor

Li J.

• College of Forestry, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China

autor

Ye X.

• College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Jinshui District, Zhengzhou 450002, China

autor

Tan B.

• College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Jinshui District, Zhengzhou 450002, China

autor

Zheng X.

• College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Jinshui District, Zhengzhou 450002, China

autor

Cheng J.

• College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Jinshui District, Zhengzhou 450002, China

autor

Wang W.

• College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Jinshui District, Zhengzhou 450002, China

autor

Wang H.

• College of Forestry, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China

autor

Gu L.

• College of Forestry, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China

autor

Feng J.

• College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Jinshui District, Zhengzhou 450002, China
• College of Forestry, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China

Bibliografia

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Identyfikatory

DOI

10.1007/s11738-019-2879-9