Identification of Scopulariopsis species by partial 28S rRNA gene sequence analysis

• Autorzy: Jagielski T. , Kosim K. , Skora M. , Macura A. B. , Bielecki J.
• Języki publikacji: EN

Abstrakty

EN

The genus Scopulariopsis contains over 30 species of mitosporic moulds, which although usually saprophytic may also act as opportunistic pathogens in humans. They have mainly been associated with onychomycosis, and only sporadically reported as a cause of deep tissue infections or systemic disease. Identification of Scopulariopsis species still largely relies on phenotype-based methods. There is a need for a molecular diagnostic approach, that would allow to reliably discriminate between different Scopulariopsis species. The aim of this study was to apply sequence analysis of partial 28S rRNA gene for species identification of Scopulariopsis clinical isolates. Although the method employed did reveal some genetic polymorphism among Scopulariopsis isolates tested, it was not enough for species delineation. For this to be achieved, other genetic loci, within and beyond the rDNA operon, need to be investigated.

Słowa kluczowe

EN

species identification; Scopulariopsis; 28S rRNA gene sequence; molecular diagnostics; mycosis; sequence analysis

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2013
• Tom: 62
• Numer: 3
• Opis fizyczny: p.303-306,fig.,ref.

Twórcy

autor

Jagielski T.

• Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland

autor

Kosim K.

• Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland

autor

Skora M.

• Chair of Microbiology, Department of Mycology, Collegium Medicum, Jagiellonian University, Krakow, Poland

autor

Macura A. B.

• Chair of Microbiology, Department of Mycology, Collegium Medicum, Jagiellonian University, Krakow, Poland

autor

Bielecki J.

• Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland

Bibliografia

• Beltrame A., L. Sarmati, L. Cudillo, R. Cerretti, A. Picardi, L. Anemona C. Fontana, M. Andreoni and W. Arcese. 2009. A fatal case of invasive fungal sinusitis by Scopulariopsis acremonium in a bone marrow transplant recipient. Int. J. Infect. Dis. 13: 488–492.
• Bontems O., P.M. Hauser and M. Monod. 2009. Evaluation of a polymerase chain reaction-restriction fragment length polymorphism assay for dermatophyte and nondermatophyte identification in onychomycosis. Br. J. Dermatol. 161: 791–796.
• Cuenca-Estrella M., A. Gomez-Lopez, E. Mellado, M.J. Buitrago, A. Monzón and J.L. Rodriguez-Tudela. 2003. Scopulariopsis brevicaulis, a fungal pathogen resistant to broad-spectrum antifungal agents. Antimicrob. Agents Chemother. 47: 2339–2341.
• de Hoog G.S., Guarro, J., Gené, J., and M.J. Figueras. 2000. Atlas of clinical fungi, 2nd ed. Centralbureau voor Schimmelcultures, Utrecht, The Netherlands.
• Isidro A.M., V. Amorosa, G.A. Stopyra, H.L. Rutenberg, W.H. Pentz and C.R.J. Bridges. 2006. Fungal prosthetic mitral valve endocarditis caused by Scopulariopsis species: case report and review of the literature. Thorac. Cardiovasc. Surg. 131: 1181–1183.
• Kriesel J.D., E.E. Adderson, W.M. Gooch and A.T. Pavia. 1994. Invasive sinonasal disease due to Scopulariopsis candida: case report and review of Scopulariopsosis. Clin. Infect. Dis. 19: 317–319.
• Larone D.H. 2002. Medically important fungi: a guide to identification, 4th ed. Washington, D.C.
• Monod M., O. Bontems, C. Zaugg, B. Léchenne, M. Fratti and R. Panizzon. 2006. Fast and reliable PCR/sequencing/RFLP assay for identification of fungi in onychomycoses. J. Med. Microbiol. 55: 1211–1216.
• Salmon A., A. Debourgogne, M. Vasbien, L. Clément, J. Collomb, F. Plénat, P. Bordigoni and M. Machouart. 2010. Disseminated Scopulariopsis brevicaulis infection in an allogeneic stem cell recipient: case report and review of the literature. Clin. Microbiol. Infect. 16: 508–512.
• Skóra M. and A.B. Macura. 2011. In vitro antifungal susceptibility testing of Scopulariopsis brevicaulis strains using agar diffusion method. Wiad. Parazytol. 57: 111–116.