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2013 | 35 | 05 |

Tytuł artykułu

Effect of bioreactor angle and aeration rate on growth and hydromechanics parameters in bioreactor culture of ginseng suspension cells

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Suspension cells of Panax ginseng C.A. Meyer were cultivated in 3-L balloon-type bubble bioreactors and the bioreactor with the angle of 90º at the bottom side was optimized. The gaseous composition in plant cell and tissue cultures is regarded as an important factor affecting the plant growth. Gas hold-up was remarkably higher in the bioreactor with an angle of 90º than the other ones. Aeration rates impacted on the growth ratio, the specific O2 uptake rate (SOUR) of ginseng cells were investigated. 0.4 vvm was selected as the optimal aeration rate with a dry weight of 6.45 g L-1. The specific O2 uptake rate in the culture time was detected and reached the top value at the maximum growth ratio.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

35

Numer

05

Opis fizyczny

p.1497-1501,fig.,ref.

Twórcy

autor
  • Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
autor
  • School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin 300072, China
autor
  • Key Laboratory of Industrial Fermentation Microbiology, Tianjin University of Science and Technology, Ministry of Education, Tianjin 300457, China
autor
  • Key Laboratory of Industrial Fermentation Microbiology, Tianjin University of Science and Technology, Ministry of Education, Tianjin 300457, China
autor
  • School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin 300072, China
autor
  • Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
autor
  • Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100193, China

Bibliografia

  • Ali MB, Yu KW, Hahn EJ, Paek KY (2005) Differential responses of anti-oxidants enzymes, lipoxygenase activity, ascorbate content and the production of saponins in tissue cultured root of mountain Panax ginseng C.A. Mayer and Panax quinquefolium L. in bioreactor subjected to methyl jasmonate stress. Plant Sci 169:83–92
  • Angelova N, Kong HW, van der Heijden R, Yang SY, Choi YH, Kim HK, Wang M, Hankemeier T, van der Greef J, Xu G, Verpoorte R (2008) Recent methodology in the phytochemical analysis of ginseng. Phytochem Anal 19:2–16
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  • Ballica R, Ryu DD (1993) Effects of rheological properties and mass transfer on plant cell bioreactor performance: production of tropane alkaloids. Biotechnol Bioeng 42:1181–1189
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  • Chinese Pharmacopoeia, 2010 (edition), p 8 Han J, Zhong JJ (2003) Effects of oxygen partial pressure on cell growth and ginsenoside and polysaccharide production in high density cell cultures of P. Notoginseng. Enzym Microb Technol 32:498–503
  • Huang T, Gao WY, Wang J, Cao Y, Zhao YX, Huang LQ, Liu CX (2010) Selection and optimization of a high-production tissue culture of Panax ginseng C.A. Meyer. Acta Physiol Plant 32:765–772
  • Jeong GT, Park DH (2005) Comparative evaluation of modified bioreactors for enhancement of growth and secondary metabolite biosynthesis using Panax ginseng hairy roots. Biotechnol Bioprocess Eng 10:528–534
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  • Paek KY, Murthy HN, Hahn EJ, Zhong JJ (2009) Large scale culture of ginseng adventitious roots for production of ginsenosides. Adv Biochem Eng Biotechnol 113:151–176
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  • Shin KS, Murthy HN, Ko JY, Paek KY (2002) Growth and betacyanin production by hairy roots of Beta vulgaris in airlift bioreactors. Biotechnol Lett 24:2067–2069
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Typ dokumentu

Bibliografia

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Identyfikator YADDA

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