Production of oleanolic acid glycosides by hairy root established cultures of Calendula officinalis L.

• Autorzy: Dlugosz M. , Wiktorowska E. , Wisniewska A. , Paczkowski C.
• Języki publikacji: EN

Abstrakty

EN

In order to initiate hairy root culture initiation cotyledons and hypocotyls of Calendula officinalis L. were infected with Agrobacterium rhizogenes strain ATCC 15834 or the same strain containing pCAMBIA 1381Z vector with β-glucuronidase reporter gene under control of promoter of NIK (Nematode Induced Kinase) gene. The efficiency of induction of hairy roots reached 33.8% for cotyledons and 66.6% for hypocotyls together for both transformation experiments. Finally, eight control and nine modified lines were established as a long-term culture. The hairy root cultures showed the ability to synthesize oleanolic acid mainly (97%) as glycosides; control lines contained it at the average 8.42 mg·g-1 dry weight in tissue and 0.23 mg·dm-3 in medium; modified lines: 4.59 mg·g-1 for the tissue, and 0.48 mg·dm-3 for the medium. Additionally lines showed high positive correlation between dry/fresh weight and oleanolic acid concentration in tissue. Using the Killiani mixture in acidic hydrolysis of oleanolic acid glycosides released free aglycones that were partially acetylated in such conditions.

Słowa kluczowe

EN

oleanolic acid glycoside; hairy root; marigold; Calendula officinalis; Agrobacterium rhizogenes; triterpenoid saponin; transformation

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2013
• Tom: 60
• Numer: 3
• Opis fizyczny: p.467-473,fig.,ref.

Twórcy

autor

Dlugosz M.

• Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, Warsaw, Poland

autor

Wiktorowska E.

• Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, Warsaw, Poland

autor

Wisniewska A.

• Department of Plant Physiology, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Warsaw, Poland

autor

Paczkowski C.

• Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, Warsaw, Poland

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