Expression of selected markers in immunohistochemical diagnosis of canine and human testicular tumours

• Autorzy: Ciaputa R. , Nowak M. , Madej J. A. , Janus I. , Gorzynska E. , Kandefer-Gola M.
• Języki publikacji: EN

Abstrakty

EN

Immunohistochemical profiles of the most common canine testicular tumours, including the Leydig cell tumours, seminomas, and Sertoli cell tumours were analysed, and the results were compared with those obtained in the corresponding txpes of human testicular neoplasms. The expressions of vimentin, von Willebrand factor (FVIII), chromogranin A, synaptophysin, and MCM3 were quantified. In the case of Sertoli cell tumours, only canine ones were analysed, since this type of tumour is very rareh diagnosed in men. The expression of the analysed proteins in the testicular tumours was similar. The von Willebrand factor exhibited the strongest expression in Lexdig cell tumours in dogs and men, while vimentin was expressed more strongly in dogs (96.7% had an intensity at +++) than in men (62.5% had +++) in the Leydigioma. The immunoexpression of MCM3 in seminomas was high in both men and dogs - 90% +++ and 100% +++ respectively. The lack of chromogranin A and synaptophysin was observed in almost 100% of seminomas in men and dogs. This differed from the results obtained for Leydigioma, where chromogranin A was expressed in 70% of dogs at +++ and in 100% of men at ++++. The results may indicate that the antibodies were selected correctly. Their anah sis and interpretation provides valuable information concerning the nature of the studied tumours.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2015
• Tom: 59
• Numer: 1
• Opis fizyczny: p.99-106,fig.,ref.

Twórcy

autor

Ciaputa R.

• Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland

autor

Nowak M.

• Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland

autor

Madej J. A.

• Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland

autor

Janus I.

• Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland

autor

Gorzynska E.

• Histopathological Laboratory Hist-Med N.Z., M. Kosinski, P. Prajs, 51-124 Wroclaw, Poland

autor

Kandefer-Gola M.

• Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland

Bibliografia

• 1. Banco B., Giudice C., Veronesi M.C., Gerosa E., Grieco V.: An immunohistochemical study of normal and neoplastic canine sertoli cells. J Comp Pathol 2010, 143, 239-247.
• 2. Ciaputa R., Nowak M., Madej J.A., Poradowski D., Janus I., Dziegiel P., Gorzynska E., Kandefer-Gola M.: Inhibin-α, E-cadherin, calretinin and Ki-67 antigen in the immunohistochemical evaluation of canine and human testicular neoplasms. Folia Histochem Cytobiol 2014, 52, 326-334.
• 3. Davidoff M.S., Schulze W., Middendorff R., Holstein A.F.: The Leydig cell of the human testis - a new member of the diffuse neuroendocrine system. Cell Tissue Res 1993, 271, 429-439.
• 4. De Lellis R.A., Shin S.J.: Immunohistology of endocrine tumors. In: Diagnostic immunohistochemistry, edited by D.J. Dabbs, Elsevier, Philadelphia, 2006, pp. 262-263.
• 5. Denis C.V.: Molecular and cellular biology of von Willebrand factor. Int J Hematol 2002, 75, 3-8.
• 6. Grieco V., Riccardi E., Greppi G.F., Teruzzi F., Iermano V., Finazzi M.: Canine testicular tumours: a study on 232 dogs. J Comp Path 2008. 138, 86-89.
• 7. Herrmann H., Aebi U.: Intermediate filaments and their associates: multi - talented structural elements specifying cytoarchitecture and cytodynamics. Curr Opin Cell Biol 2000. 12, 79-90.
• 8. Kennedy P.C., Cullen J.M., Edwards J.F., Goldschmidt M.H., Larsen S., Munson L., Nielsen S.: Histological Classification of Tumors of the Genital System of Domestic Animals. Armed Forces Institute of Pathology, Washington, 1998, pp. 12-20.
• 9. Kuroda N., Senzaki T., Yamasaki Y., Miyazaki E., Hayashi Y., Toi M., Hiroi M., Enzan H.: Sertoli cell tumor of the testis (not otherwise specified) with the expression of neuroendocrine markers and without the expression of inhibin-alpha. Pathol Int 2004, 54, 719-724.
• 10. Kwon S.E., Chapman E.R.: Synaptophysin regulates the kinetics of synaptic vesicle endocytosis in central neurons. Neuron 2011, 70, 847-885.
• 11. Lei M., Tye B.K.: Initiating DNA synthesis: from recruiting to activating the MCM complex. J Cell Sci 2001, 114, 1447-1454.
• 12. Levin H.S.: Neoplasms of the testis. In: Genitourinary Pathology: A Volume in Foundations in Diagnostic Pathology Series, edited by M. Zhou, C. Magi-Galluzzi, Elsevier, Philadelphia, 2007, pp. 582-588.
• 13. Mooney E.E., Nogales F.F., Bergeron C., Tavassoli F.A.: Retiform Sertoli-Leydig cell tumours: clinical, morphological and immunohistochemical findings. Histopathology 2002, 41, 110-117.
• 14. Padró T., Ruiz S., Bieker R., Bürger H., Steins M., Kienast J., Büchner T., Berdel W.E., Mesters R.M.: Increased angiogenesis in the bone marrow of patients with acute myeloid leukemia. Blood 2000, 95, 2637-2644.
• 15. Remmele W., Stegner H.E.: Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue. Pathologie. 1987, 8, 138-140.
• 16. Reyes A., Moran C.A., Suster S., Michal M., Dominguez H.: Neuroendocrine carcinomas (carcinoid tumor) of the testis. A clinicopathologic and immunohistochemical study of ten cases. Am J Clin Pathol 2003, 120, 182-187.
• 17. Saegusa Y., Hayashi H., Taniai E., Imaoka M., Ohishi T., Wang L., Mitsumori K., Shibutani M.: Spermatocytic seminoma with neuroectodermal differentiation and sertoli cell tumor in a dog. Vet Pathol 2011, 48, 1024-1028.
• 18. Tye B.K., Sawyer S.: The hexametric eukaryotic MCM helicase: building symmetry from non identical parts. J Biol Chem 2000, 275, 34833-34836.
• 19. Wang Z., Yang S., Shi H., Du H., Xue L., Wang L., Dong Y., Han A.: Histopathological and immunophenotypic features of testicular tumour of the adrenogenital syndrome. Histopathology 2011, 58, 1013-1018.
• 20. Werner M., Kaloutsi V., Walter K.., Buhr T., Bernhards J., Georgii A.: Immunohistochemical examination of routinely processed bone marrow biopsies. Pathol Res Pract 1992, 188, 707-713.

Identyfikatory

DOI

10.1515/bvip-2015-0015