Effect of pretilachlor on nitrogen uptake and assimilation by the cyanobacterium Desmonostoc muscorum PUPCCC 405.10

• Autorzy: Singh D.P. , Khattar J.I.S. , Kaur G. , Gupta M. , Singht Y. , Gulati A.
• Języki publikacji: EN

Abstrakty

EN

Tolerance limit, kinetics of nitrogen uptake and activity of nitrate reductase (NR), nitrite reductase (NiR) and glutamine synthetase (GS) enzymes of cyanobacterium Desmonostoc muscorum PUPCCC 405.10 were studied under the regime of chloroacetanilide herbicide pretilachlor. The organism was isolated from the paddy field under application of the herbicide and tolerated pretilachlor up to 10 ppm under laboratory conditions. The incubation of the cyanobacterium in 2.5 ppm pretilachlor did not cause significant effect on N uptake while supplementation of 5–10 ppm pretilachlor in medium reduced the rate of nitrate and nitrite uptake by 35–73 % with 50 % decrease in Vmax and no change in Km. These results indicated that herbicide did not affect the affinity of uptake system to nitrate and nitrite. In presence of herbicide, the activity of nitrogen assimilation enzymes was inhibited by 16 % for NR and 18 % for NiR. Unchanged Km and decreased Vmax (50–60 %) in presence of herbicide indicated non-competitive- type inhibition of the enzymes.A50 %decrease in Vmax and Km of ammonium uptake indicated un-competitive type inhibition of the ammonium transport system by the herbicide. GS activity also exhibited non-competitive inhibition in presence of pretilachlor with 21 % decrease in activity with unchanged Km and 40 % decrease in Vmax. Likewise, decreases in nitrogenase activity by 43 % and heterocyst formation by 40 % were recorded in presence of herbicide. Results indicated that pretilachlor affected nitrogen assimilation at uptake level and interacted with nitrogen-assimilating enzymes in a non-competitive manner.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 09
• Opis fizyczny: Article: 177 [14 p.], fig.,ref.

Twórcy

autor

Singh D.P.

• Department of Botany, Punjabi University, Patiala, Punjab, 147002, India

autor

Khattar J.I.S.

• Department of Botany, Punjabi University, Patiala, Punjab, 147002, India

autor

Kaur G.

• Department of Botany, Punjabi University, Patiala, Punjab, 147002, India

autor

Gupta M.

• Department of Botany, Punjabi University, Patiala, Punjab, 147002, India

autor

Singht Y.

• Department of Botany, Punjabi University, Patiala, Punjab, 147002, India

autor

Gulati A.

• Plant Pathology and Microbiology Laboratory, CSIR-Institute of Himalayan Bioresource Technology, Post Box No. 6, Palampur, Himachal Pradesh, 176 061, India

Bibliografia

• Adhya TK, Rao VR, Ramakrishan B, Sethunathan N (2000) Pesticide and Soil health: problems and perspective. In: Dhailwal GS, Singh B (eds) Pesticide and Environment Commonwealth Press, New Delhi, pp 128
• Ahluwalia AS, Dahuja S (1997) Toxicity of a carbamate pesticide in a nitrogen fixing algae Nostoc muscorum Ag. ex Born. et Flah. In: Grover IS, Thukral AK (eds) Environment and Development. Thukral Scientific Publication, Jodhpur, pp 199–204
• Bagchi SN, Tatjana B, Pistorius EK, Michel KP (2007) A Synechococcus elongatus PCC 7942 mutant with a higher tolerance towards bentazone also confers resistance to sodium chloride stress. Photosynth Res 92:87–101
• Begam ZNT, Mandal R, Islam S (2011) Effect of cyanobacterial biofertilizer on the growth and yield components of two hyv of rice. J Algal Biomass Utln 2:1–9
• Bhunia AK, Marik R, Banerjee SK (1994) Biochemical effects of carbaryl on nitrogen assimilating enzymes of cyanobacterium Nostoc muscorum. Bull Environ Contam Toxicol 52:886–892
• Broser M, Glöckner C, Gabdulkhakov A, Guskov A, Buchta J, Kern J, Müh F, Dau H, Saenger W, Zouni A (2011) Structural basis of cyanobacterial photosystem II inhibition by the herbicide terbutryn. J Biol Chem 286:15964–15972
• Chen Z, Juneau P, Qiu B (2007) Effects of three pesticides on the growth, photosynthesis and photoinhibition of the edible cyanobacterium Ge-Xian-Mi (Nostoc). Aquat Toxicol 81:256–265
• Desikachary TV (1959) Cyanophyta. Indian council of agricultural research, New Delhi
• Dowidar SMA, Osman MEH, El-Naggar AH, Khalefa AE (2010) Effect of butachlor and thiobencarb herbicides on protein content and profile and some enzyme activities of Nostoc muscorum. J Genet Engg Biotechnol 8:89–95
• El-Shahed AM (2005) Interaction of 2,4-D with a nitrogen fixing Nostoc rivulare Kutzing associated with wheat plants. Bull Fac Sci Assiut Univ 15:123–132
• El-Shahed AM, Abdel-Wahab MA (2006) Para-nodule induction in wheat, maize and rice with 2,4-D and its infection with Nostoc rivulare Kutzing. Pak J Biol Sci 9:1693–1699
• Fadl-Allah ME, El-komy HM, Sholkamy NE (2010) The effect of cyanobacteria, Nostoc muscorum as biofertilizer on growth and N-yield of wheat (Triticum aestivum L.) under different doses of N-fertilizer. J Assoc Arab Univ Basic Appl Sci Bahrain 8:61–74
• Fernández-Valiente E, Ucha A, Quesada A, Leganés F, Carreres R (2000) Contribution of N2 fixing cyanobacteria to rice production: availability of nitrogen from 15N-labelled cyanobacteria and ammonium sulphate to rice. Plant Soil 221:107–112
• Flores E, Herrero A (2005) Nitrogen assimilation and nitrogen control in cyanobacteria. Biochem Soc Transac 33:164–167
• Flores E, Ramos JL, Herrero A, Guerrero MG (1983) Nitrate assimilation by cyanobacteria. In: Packer GC, Papageorgiou L (eds) Photosynthetic prokaryotes: cell differentiation and function. Elsevier Amsterdam, The Netherlands, pp 363–387
• Flores E, Erias JE, Rubio LM, Herrero A (2005) Photosynthetic nitrate assimilation in cyanobacteria. Photosynth Res 83:117–133
• Forlani G, Pavan M, Gramek M, Kafarski P, Lipok J (2008) Biochemical basis for a wide spread tolerance of cyanobacteria to the phosphonate herbicide glyphosate. Plant Cell Physiol 49:443–456
• Galhano V, Peixoto F, Gomes-Laranjo J, Fernández-Valiente E (2010a) Comparative toxicity of bentazon and molinate on growth, photosynthetic pigments, photosynthesis and respiration of the Portuguese rice field cyanobacterium Nostoc muscorum. Environ Toxicol 25:147–156
• Galhano V, Peixoto F, Gomes-Laranjo J (2010b) Bentazon triggers the promotion of oxidative damage in the Portuguese ricefield cyanobacterium Anabaena cylindrica: response of the antioxidant system. Environ Toxicol 25:517–526
• Galhano V, Gomes-Laranjo J, Peixoto F (2011) Exposure of the cyanobacterium Nostoc muscorum from Portuguese rice fields to molinate (Ordram): effects on the antioxidant system and fatty acid profile. Aquat Toxicol 101:367–376
• Gill MS, Bajwa HS (2012) Package of practices for crops of Punjab (Kharif-2012). Punjab Agricultural University, Ludhiana, India
• Herrero A, Flores E, Guerrero MG (1981) Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp. strain 7119 and Nostoc sp. strain 6719. J Bacteriol 145:175–180
• Hrouzek P, Lukešová A, Mareš J, Ventura S (2013) Description of the cyanobacterial genus Desmonostoc gen. nov. including D. muscorum comb. nov. as a distinct, phylogenetically coherent taxon related to the genus Nostoc. Fottea 13:201–213
• Inderjit Kaushik S (2010) Effect of herbicide with different modes of action on physiological and cellular traits of Anabaena fertilissima. Paddy Water Environ 8:277–282
• Irisarri P, Gonnet S, Monza J (2001) Cyanobacteria in Uruguayan rice fields: diversity, nitrogen fixing ability and tolerance to herbicides and combined nitrogen. J Biotechnol 91:95–103
• Jha MN, Mishra SK (2005) Biological response of cyanobacteria to insecticides and their insecticide degrading potential. Bull Environ Contam Toxicol 75:374–381
• Kannaiyan S (1990) Blue green algal biofertilizer for rice. In: Kannaiyan S (ed) Biotechnology of biofertilizers for rice crop. TNAU Publication, Coimbatore, India, p 212
• Kaur M, Ahluwalia AS, Dahuja S (2002) Toxicity of a rice field herbicide in a nitrogen fixing alga Cylindrospermum sp. J Environ Biol 23:359–363
• Kim JM, Lee CG (2006) Differential response of two fresh water cyanobacteria, Anabaena variabilis and Nostoc commune to sulfonylurea herbicide bensulfuron methyl. J Microbiol Biotechnol 16:52–56
• Komárek J, Anagnostidis K (1989) Modern approach to the classification system of cyanophytes. 4: nostocales. Arch Hydrobiol/Algol Stud 56:247–345
• Komárek J, Hauer T (2013) Cyanodb.cz Online database of cyanobacterial genera. Word-wide electronic publication, Univ. of South Bohemia and Institute of Botany AS CR
• Kumar NJI, Kumar RN, Bora A, Amb MK (2012) Differential effects of agricultural pesticides endosulfan and tebuconazole on photosynthetic pigments, metabolism and assimilating enzymes of three heterotrophic, filamentous cyanobacteria. J Biol Environ Sci 6:67–75
• Lange OL, Kilian E, Zeigler H (1994) Water vapour uptake and photosynthesis of lichens: performance difference in species with green and blue green algae as phycobionts. Oecologia 71:425–437
• Leganés F, Fernández-Valiente E (1992) Effects of phenoxy acetic herbicides on growth, photosynthesis and nitrogenase activity in cyanobacteria from rice fields. Arch Environ Contam Toxicol 22:130–134
• López-Rodas V, Flores-Moya A, Manerio E, Perdigones N, Marva F, García ME, Costas E (2007) Resistance to glyphosate in the cyanobacterium Microcystis aeruginosa as result of pre selective mutations. Evol Ecol 21:535–547
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
• Mallory-Smith CA, Retzinger EJ Jr (2003) Revised classification of herbicides by site of action for weed resistance management strategies. Weed Technol 17:605–619
• Marco E, Orus MI (1993) Trichlorfon-induced inhibition of nitrate and ammonium uptake in cyanobacteria. J Exp Bot 44:501–508
• Mishra U, Pabbi S (2004) Cyanobacteria a potential biofertilizer for rice. Resonance 9:6–10
• Montesinos MS, Muro-Pastor AM, Herrero A, Flores E (1998) Ammonium/methylammonium permeases of a cyanobacterium. Identification and analysis of three nitrogen-regulated amt genes in Synechocystis sp. PCC 6803. J Biol Chem 273:31463–31470
• Narusaka Y, Narusaka M, Kobayashi H, Satoh K (1998) The herbicide-resistant species of the cyanobacterial Dl protein obtained by thorough and random in vitro mutagenesis. Plant Cell Physiol 39:620–626
• Nicholas DJ, Nason A (1957) Determination of nitrate and nitrite. Methods Enzymol 3:981–984
• Nübel U, Garcia-Pichel F, Muyzer G (1997) PCR primers to amplify 16S rRNA genes from cyanobacteria. Appl Environ Microbiol 63:3327–3332
• Okmen G, Ugur A (2011) Influence of bispyribac sodium on nitrogenase activity and growth of cyanobacteria isolated from paddy fields. African J Microbiol Res 5:2760–2764
• Okmen G, Turkcan O, Erdal P (2013) Effect of herbicides on chlorophyll-a, β- carotene, phycocyanin and allophycocyanin content of Anabaena sp. J Appl Biol Sci 7:20–27
• Pandey FK, Kumar S, Bhatnagar T (2011) Toxic effect of an herbicide on growth and heterocyst formation of two N2-fixing cyanobacteria. Intel J Curr Res 3:14–16
• Roberts TR (1998) Chloroacetanilides. In: Roberts TR, Hutson DH, Lee PW, Nicholls PH, Plimmer JR, Roberts MC, Croucher L (eds) Metabolic pathways of agrochemicals part 1: herbicides and plant growth regulators, Royal society of chemistry, pp 179–208
• Robinson JBD, de Allen MV, Gacoka P (1959) The determination of soil nitrates with a brucine reagent. Analyst 84:635–640
• Roger PA, Ladha JK (1992) Biological N2 fixation in wetland rice fields: estimation and contribution to nitrogen balance. Plant Soil 141:41–55
• Safferman RS, Morris ME (1964) Growth characteristics of the blue green algal virus LPP-1. J Bacteriol 88:771–775
• Shapiro BM, Stadtman ER (1970) Glutamine synthetase of Escherchia coli. In: Tabor H, Tabor CW (eds) Methods of enzymology, vol XVII A. Academic Press, New York, pp 910–922
• Shen J, Jiang J, Zheng P (2009) Effects of light and monosulfuron on growth and photosynthetic pigments of Anabaena flos-aquae Breb. J Water Resour Prot 1:408–413
• Sholkamy EN, El-Komy H, Al-Arfaj A, Abdel-Megeed A, Mostafa AA (2012) Potential role of Nostoc muscorum and Nostoc rivulare as biofertilizers for the enhancement of maize growth under different doses of N-fertilizer. Afr J Microbiol Res 6:7435–7448
• Singh S, Datta P (2006) Screening and selection of most potent diazotrophic cyanobacterial isolate exhibiting natural tolerance to rice field herbicides for exploration as biofertilizers. J Basic Microbiol 46:219–225
• Singh S, Datta P (2007) Outdoor evaluation of herbicide resistant strains of Anabaena variabilis as biofertilizer for rice plants.Plant Soil 296:95–102
• Singh DP, Sandhu BS (2010) Effect of anilofos on growth, photosynthetic pigments and stress enzymes of cyanobacterium Oscillatoria simplicissima. Res J Biotechnol 5:27–32
• Singh DP, Khattar JIS, Nadda J, Singh Y, Garg A, Kaur N, Gulati A (2011) Chlorpyrifos degradation by the cyanobacterium Synechocystis sp. strain PUPCCC 64. Environ Sci Pollut Res 18:1351–1359
• Singh DP, Khattar JIS, Kaur K, Sandhu BS, Singh Y (2012) Toxicological impact of anilofos on some physiological processes of a rice field cyanobacterium Anabaena torulosa. Toxicol Environ Chem 94:1304–1318
• Singh DP, Khattar JIS, Kaur M, Kaur G, Gupta M, Singh Y (2013) Anilofos tolerance and its mineralization by the cyanobacterium Synechocystis sp. strain PUPCCC 64. PLoS One 8:e53445. doi:10.1371/journal.pone.0053445
• Solarzano L (1969) Determination of ammonia in natural waters by the phenol hypochlorite method. Limnol Oceang 14:799–801
• Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue green algae (order Chroococcales). Bacteriol Rev 35:171–205
• Stewart WDP, Fitzgerald GP, Burris RH (1968) Acetylene reduction by nitrogen fixing blue-green algae. Arch Microbiol 62:336–348
• Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
• Tiwari AK, Kumar A, Mishra AK (1991) Use of cyanobacterial diazotrophic technology in rice agriculture. Appl Biochem Biotechnol 28:387–396
• Tomitani A, Knoll AH, Cavanaugh CM, Ohno T (2006) The evolutionary diversification of cyanobacteria: molecular—phylogenetic and paleontological perspectives. Proc Natl Acad Sci 103:5442–5447
• Way MJ (1976) Entomology and the world food situation. Bull Entomol Soc Am 22:125–129
• Whitton BA, Potts M (2000) Introduction to cyanobacteria. In: Whitton BA, Potts M (eds) The ecology of cyanobacteria: their diversity in time and space, Kluwer Academic Press, The Netherlands, pp 1–11
• Yaduraju NT (2003) Potential losses caused due to weeds in some selected crops in India. In: Saraswat VN, Bhan VM, Yaduraju NT (eds) Weed Management. ICAR, New Delhi, p 3

Identyfikatory

DOI

10.1007/s11738-015-1923-7