Use of silver nitrate for the assessment of sperm measurements in selected farm and free - living animal species

• Autorzy: Andraszek K. , Banaszewska D. , Szostek M. , Wojcik E. , Czubaszek M. , Walczak-Jedrzejowska R.
• Języki publikacji: EN

Abstrakty

EN

The study was conducted on spermatozoa of selected farm and free-living animal species, isolated post mortem from the tail of the epididymis, and stained with silver nitrate - AgNO₃. The material was collected from pigs, goats, wild boar, and European roe deer. Twenty morphologically normal spermatozoa randomly selected from each animal and well visible under the microscope, were analysed. The following measurements were considered: head length, width, perimeter and area, acrosome area, mid-piece length, tail length, and overall sperm length. AgNO₃ staining differentiated the acrosomal (light hue) and distal (dark hue) part of the sperm head, and a light-hued mid-piece was visible within the sperm tail. Silver nitrate staining revealed species and variety-related differences, particularly in reference to the sperm head. Clear-cut differentiation within the head and tail area made it possible to perform detailed morphometric measurements of the spermatozoa.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2014
• Tom: 58
• Numer: 3
• Opis fizyczny: p.487-494,fig.,ref.

Twórcy

autor

Andraszek K.

• Department of Animal Genetics and Horse Breeding, Institute of Bioengineering and Animal Breeding, Siedlce University of Natural Sciences and Humanities, 08-110, Siedlce, Poland

autor

Banaszewska D.

• Department of Breeding Methods and Poultry and Small Ruminant Breeding, Institute of Bioengineering and Animal Breeding, Siedlce University of Natural Sciences and Humanities, 08-110, Siedlce, Poland

autor

Szostek M.

• Department of Animal Genetics and Horse Breeding, Institute of Bioengineering and Animal Breeding, Siedlce University of Natural Sciences and Humanities, 08-110, Siedlce, Poland

autor

Wojcik E.

• Department of Animal Genetics and Horse Breeding, Institute of Bioengineering and Animal Breeding, Siedlce University of Natural Sciences and Humanities, 08-110, Siedlce, Poland

autor

Czubaszek M.

• Department of Animal Genetics and Horse Breeding, Institute of Bioengineering and Animal Breeding, Siedlce University of Natural Sciences and Humanities, 08-110, Siedlce, Poland

autor

Walczak-Jedrzejowska R.

• Department of Andrology and Reproductive Endocrinology, Medical University in Lodz, 91-425, Lodz, Poland

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Identyfikatory

DOI

10.2478/bvip-2014-0074