Arc functional neighbourhood in the neuronal cell nucleus

• Autorzy: Parobczak K. , Rutkowska-Wlodarczyk L. , Szczepankiewicz A. A. , Khanema-Jakobsen T. , Schubert M. , Bramham C. R. , Wilczynski G. M.
• Języki publikacji: EN

Abstrakty

EN

Arc protein is a versatile factor connecting memory formation, plasticity changes and neuronal activity. Through regulation of actin polymerization, Arc contributes to synapse expansion and may furthermore influence synapse strength via management of AMPA receptor turnover. The function of Arc in the neuronal cell nucleus is poorly understood. In this work we performed structural, functional and biochemical analysis to identify Arc`s nuclear interactome. Using confocal microscopy we investigated Arc`s functional neighborhood and found that it occupied internal parts of the nucleus, closely to hnRNPs. This observation were confirmed with electron microscopy, which demonstrated that Arc localizes mainly at the peripheral areas of chromatin. Furthermore, pull-down-based biochemical experiments suggested that Arc interacts with splicing machinery. Collectively, our data suggest that nuclear Arc is involved in the gene expression phenomena.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: Suppl.1
• Opis fizyczny: p.28

Twórcy

autor

Parobczak K.

• Deptartment of Neurophysiology, Nencki Insitute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Rutkowska-Wlodarczyk L.

• Deptartment of Molecular and Cellular Neurobiology, Nencki Insitute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Szczepankiewicz A. A.

• Deptartment of Neurophysiology, Nencki Insitute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland

autor

Khanema-Jakobsen T.

• Department. of Biomedicine, Jebsen Centre for Research on Neuropsychiatric Disorders, University of Bergen, Bergen, Norway

autor

Schubert M.

• Department. of Biomedicine, Jebsen Centre for Research on Neuropsychiatric Disorders, University of Bergen, Bergen, Norway

autor

Bramham C. R.

• Department. of Biomedicine, Jebsen Centre for Research on Neuropsychiatric Disorders, University of Bergen, Bergen, Norway

autor

Wilczynski G. M.

• Deptartment of Neurophysiology, Nencki Insitute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland