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2011 | 71 | S |
Tytuł artykułu

Microfabrication techniques and surface functionalisation for the culture of neural stem cells

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
We present different processes used for the patterning and growth of stem cells based on microspotting, microcontact printing and 3D patterning. Microspotting and microcontact-printing technique have been performed to produce micropatterned surfaces for cell-biological applications. Biomolecules have been micropsotted and microstamped on plasma-polymerized poly ethylene glycol substrates. The patterns exhibited a firm stability and an improved feasibility for controlling cell localization, proliferation and even differentiation. Production and application of 3D substrate by combination of lithography and in situ UV cross linking of photoresist are also detailed. An application of these processes is the biofunctional surfaces were incubated with suspensions of human umbilical cord blood neural stem cells (HUCB-NSC). It was clearly observed how the cells adhered and grew in the protein patterned regions. After 4 weeks of culturing the cells were still anchored in the patterns. Immunocytochemistry studies indicate that cell differentiation can be controled by the combination of interface engineering and culture conditions.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
71
Numer
S
Opis fizyczny
p.19
Twórcy
autor
  • Joint Research Centre, IHCP, Brussels, Belgium
autor
  • Joint Research Centre, IHCP, Brussels, Belgium
autor
  • Joint Research Centre, IHCP, Brussels, Belgium
autor
  • Joint Research Centre, IHCP, Brussels, Belgium
autor
  • Department of Pharmacology, Chemotherapy and Medical Toxicology, University of Milan, Milan, Italy
Bibliografia
Uwagi
Rekord w opracowaniu
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-b4cd81dd-ada9-488e-9c76-5905ecfa5698
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