The role of PnACO1 in light- and IAA-regulated flower inhibition in Pharbitis nil

• Autorzy: Wilmowicz E. , Frankowski K. , Kesy J. , Glazinska P. , Wojciechowski W. , Kucko A. , Kopcewicz J.
• Języki publikacji: EN

Abstrakty

EN

In this study, the first ACC oxidase (PnACO1) cDNA from model short-day plant Pharbitis nil was isolated. The expression pattern of PnACO1 was studied under different conditions (photoperiod and auxin), an adequate balance of which determines P. nil flowering. It was shown that the gene was transcribed in all the examined organs of the 5-day-old seedling and was strongly activated by auxin. Our results also revealed that PnACO1 transcript accumulation in the cotyledons showed diurnal oscillations under both LD and SD conditions. On the basis of presented and previously obtained data, we suggest that flowering inhibition evoked by IAA in P. nil results from its stimulatory effect on both ACC synthase and oxidase gene expression and, consequently, enhances ethylene production.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 03
• Opis fizyczny: p.801-810,fig.,ref.

Twórcy

autor

Wilmowicz E.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Torun, Poland

autor

Frankowski K.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Torun, Poland

autor

Kesy J.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Torun, Poland

autor

Glazinska P.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Torun, Poland

autor

Wojciechowski W.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Torun, Poland

autor

Kucko A.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Torun, Poland

autor

Kopcewicz J.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Torun, Poland

Bibliografia

• Abel S, Nguyen MD, Chow W, Theologis A (1995) ACS4, a primary indoleacetic acid-responsive gene encoding 1-aminocyclopropane-1-carboxylate synthase in Arabidopsis thaliana. J Biol Chem 270:19093–19099
• Amagasa T, Suge H (1987) The mode of flower inhibiting action of ethylene in Pharbitis nil. Plant Cell Physiol 28:1159–1161
• Arteca JM, Arteca RN (1999) A multi-responsive gene encoding 1-aminocyclopropane-1-carboxylate synthase (ACS6) in mature Arabidopsis leaves. Plant Mol Biol 39:209–219
• Bleecker AB, Kende H (2000) Ethylene: a gaseous signal molecule in plants. Annu Rev Cell Dev Biol 16:1–18
• Blume B, Grierson D (1997) Expression of ACC oxidase promoter–GUS fusions in tomato and Nicotiana plumbaginifolia regulated by developmental and environmental stimuli. Plant J 12:731–746
• Bodson M (1985) Sinapis alba. In: Halevy AH (ed) Handbook of flowering, vol IV. CRC Press, Boca. Raton, pp 336–354
• Bouquin T, Lasserre E, Pradier J, Pech JC, Balague C (1997) Wound and ethylene induction of the ACC oxidase melon gene CMACO1 occurs via two direct and independent transduction pathways. Plant Mol Biol 35:1029–1035
• Finlayson SA, Lee IJ, Mullet JE, Morgan PW (1999) The mechanism of rhythmic ethylene production in Sorghum. The role of phytochrome B and simulated shading. Plant Physiol 119:1083–1089
• Frankowski K, Ke˛sy J, Wojciechowski W, Kopcewicz J (2009) Lightand IAA-regulated ACC synthase gene (PnACS) from Pharbitis nil and its possible role in IAA-mediated flower inhibition. J Plant Physiol 166:192–202
• Friedman H, Spiegelstein H, Goldschmidt EE, Halevy AH (1990) Flowering response of Pharbitis nil to agents affecting cytoplasmic pH. Plant Physiol 94:114–119
• Galoch E, Burkacka-Łałkajtys E, Kopcewicz J (1996) Effect of cytokinins on flower differentiation in cultured plantlets of Pharbitis nil Chois. Acta Physiol Plant 18:223–227
• Galoch E, Czaplewska J, Burkacka-Łałkajtys E, Kopcewicz J (2002) Induction and stimulation of in vitro flowering of Pharbitis nil by cytokinin and gibberellin. Plant Growth Regul 37:199–205
• Groenewald EG, van der Westhuizen AJ (2001) The flowering stimulus and possible involvement of prostaglandins in the flowering of Pharbitis nil. S Afr J Sci 97:313–317
• Halevy AH, Spiegelstein H, Goldschmidt EE (1991) Auxin inhibition of flower induction of Pharbitis nil is not mediated by ethylene. Plant Physiol 95:652–654
• Hudgins JW, Ralph SG, Franceschi VR, Bohlmann J (2006) Ethylene in induced conifer defense: cDNA cloning, protein expression, and cellular and subcellular localization of 1-aminocyclopropane-1-carboxylate oxidase in resin duct and phenolic parenchyma cells. Planta 224:865–877
• Hunter DA, Yoo SD, Butcher SM, McManus MT (1999) Expression of 1-aminocyclopropane-1-carboxylate oxidase during leaf ontogeny in white clover. Plant Physiol 120:131–141
• Kathiresan A, Reid DM, Chinnappa CC (1996) Light-entrained and temperature-entrained circadian regulation of activity and messenger RNA accumulation of 1-aminocyclopropane-1-carboxylic acid oxidase in Stellaria longipes. Planta 199:329–335
• Kathiresan A, Nagarathna KC, Moloney MM, Reid DM, Chinnappa CC (1998) Differential regulation of 1-aminocyclopropane-1-carboxylate synthase gene family and its role in phenotypic plasticity in Stellaria longipes. Plant Mol Biol 36:265–274
• Ke˛sy J, Trzaskalska A, Galoch E, Kopcewicz J (2003) Inhibitory effect of brassinosteroids on the flowering of the short-day plant Pharbitis nil. Biol Plant 47:597–600
• Ke˛sy J, Maciejewska B, Sowa M, Szumilak M, Kawałowski K, Borzuchowska M, Kopcewicz J (2008) Ethylene and IAA interactions in the inhibition of photoperiodic flower induction of Pharbitis nil. Plant Growth Regul 55:43–50
• Ke˛sy J, Frankowski K, Wilmowicz E, Glazin´ska P, Wojciechowski W, Kopcewicz J (2010) The possible role of PnACS2 in IAAmediated flower inhibition in Pharbitis nil. Plant Growth Regul 61:1–10
• Kieber JJ, Rothenberg M, Roman G, Feldmann KA, Ecker JR (1993) CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases. Cell 72:427–441
• Kim YS, Choi D, Lee MM, Lee SH, Kim WT (1998) Biotic and abiotic stress-related expression of 1-aminocyclopropane-lcarboxylate oxidase gene family in Nicotiana glutinosa L. Plant Cell Physiol 39:565–573
• King RW, Pharis RP, Mander LN (1987) Gibberellins in relation to growth and flowering in Pharbitis nil. Plant Physiol 84:1126–1131
• Kulikowska-Gulewska H, Cymerski M, Czaplewska J, Kopcewicz J (1995) IAA in the control of photoperiodic flower induction of Pharbitis nil Chois. Acta Soc Bot Pol 64:45–50
• Lee SH, Reid DM (1996) The role of endogenous ethylene in the expansion of Helianthus annuus leaves. Can J Bot 75:501–508
• Liu JH, Lee-Tamon SH, Reid DM (1997) Differential and woundinducible expression of 1-aminocyclopropane-1-carboxylate oxidase genes in sunflower seedlings. Plant Mol Biol 34:923–933
• Machackova I, Chauvaux N, Dewitte W, van Onkelen H (1997) Diurnal fluctuations in ethylene formation in Chenopodium rubrum. Plant Physiol 113:981–985
• Maciejewska BD, Kopcewicz J (2003) Inhibitory effect of methyl jasmonate (JA-Me) on flowering and elongation growth in Pharbitis nil. J Plant Growth Regul 21:216–223
• Nie X, Singh RP, Tai GCC (2001) Molecular characterization and expression analysis of 1-aminocyclopropane-1-carboxylate oxidase homologs from potato under abiotic and biotic stresses. Genome 45:905–913
• Ogawa Y, Zeevaart JAD (1967) The relation of growth regulators to flowering: auxin. In: Imamura S (ed) Physiology of flowering in Pharbitis nil. Japanese Society of Plant Physiologists, Kyoto, pp 107–108
• Osborne DJ (1991) Ethylene in leaf ontogeny and abscission. In: Mattoo AK, Suttle JC (eds) The plant hormone ethylene. CRC Press, Boca Raton, pp 193–214
• Overmyer K, Tuominen H, Kettunen R, Betz C, Langebartels C, Sandermann HJ, Kangasja¨rvi J (2000) Ozone-sensitive Arabidopsis rcd1 mutant reveals opposite roles for ethylene and jasmonate signaling pathways in regulating superoxide dependent cell death. Plant Cell 12:1849–1862
• Peck SC, Kende H (1995) Sequential induction of the ethylene biosynthetic enzymes by indole-3-acetic acid in etiolated peas. Plant Mol Biol 28:293–301
• Peck SC, Kende H (1998) Differential regulation of genes encoding 1-aminocyclopropane-1-carboxylate (ACC) synthase in etiolated pea seedlings: effects of indole-3-acetic acid, wounding, and ethylene. Plant Mol Biol 38:977–982
• Petruzzelli L, Coraggio I, Leubner-Metzger G (2000) Ethylene promotes ethylene biosynthesis during pea seed germination by positive feedback regulation of 1-aminocyclo-propane-1-carboxylic acid oxidase. Planta 211:144–149
• Pidgeon CM, Reid DM, Facchini PJ (1997) Light-induced changes in ethylene production in Phaseolus vulgaris cv. Taylor (abstract no. 801). Plant Physiol 114:S–165
• Smith JM, Arteca RN (2000) Molecular control of ethylene production by cyanide in Arabidopsis thaliana. Physiol Plant 109:180–187
• Song JD, Lee DH, Rhew TH, Lee CH (2003) Effects of light on the expression of 1-aminocyclopropane-1-carboxylic acid synthase and oxidase genes in mung bean hypocotyls. J Photosci 10:189–193
• Song JD, Kim JH, Lee DH, Rhew TH, Cho SH, Lee CH (2005) Developmental regulation of the expression of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase genes in hypocotyls of etiolated mung bean seedling. Plant Sci 168:1149–1155
• Tian Q, Uhlir NJ, Reed JW (2002) Arabidopsis SHY2/IAA3 inhibits auxin regulated gene expression. Plant Cell 14:301–319
• Tsuchisaka A, Theologis A (2004a) Heterodimeric interactions among the 1-amino-cyclopropane-1-carboxylate synthase polypeptides encoded by the Arabidopsis gene family. Proc Natl Acad Sci USA 101:2275–2280
• Tsuchisaka A, Theologis A (2004b) Unique and overlapping expression patterns among the Arabidopsis 1-aminocyclopropane-1-carboxylate synthase gene family members. Plant Physiol 136:2982–3000
• Vriezen WH, Hulzink R, Mariani C, Voesenek LACJ (1999) 1-aminocyclopropane-1-carboxylate oxidase activity limits ethylene biosynthesis in Rumex palustris during submergence. Plant Physiol 121:189–195
• Wijayanti L, Fujioka S, Kobayashi M, Sakurai A (1996) Effect of uniconazole and gibberellin on the flowering of Pharbitis nil. Biosci Biotech Biochem 60:852–855
• Wijayanti L, Fujioka S, Kobayashi M, Sakurai A (1997) Involvement of abscisic acid and indole-3-acetic acid in the flowering of Pharbitis nil. J Plant Growth Regul 16:115–119
• Wilmowicz E, Ke˛sy J, Kopcewicz J (2008) Ethylene and ABA interactions in the regulation of flower induction in Pharbitis nil. J Plant Physiol 165:1917–1928
• Yamagami T, Tsuchisaka A, Yamada K, Haddon WF, Harden LA, Theologis A (2003) Biochemical diversity among the 1-aminocyclopropane-1-carboxylate synthase isozymes encoded by the Arabidopsis gene family. J Biol Chem 278:49102–49112
• Yoo A, Seo YS, Jung JW, Sung SK, Kim WT, Lee W, Yang DR (2006) Lys296 and Arg299 residues in the C-terminus of MDACO1 are essential for a 1-aminocyclopropane-1-carboxylate oxidase enzyme activity. J Struct Biol 156:407–420
• Yu SJ, Kim S, Lee JS, Lee DH (1998) Differential accumulation of transcripts for ACC synthase and ACC oxidase homologs in etiolated mung bean hypocotyls in response to various stimuli. Mol Cells 30:350–358
• Zanetti ME, Terrile MC, Arce D, Godoy AV, Segundo BS, Casalongue´ C (2002) Isolation and characterization of a potato cDNA corresponding to a 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene differentially activated by stress. J Exp Bot 53:2455–2457

Uwagi

rekord w opracowaniu