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2015 | 18 | 1 |

Tytuł artykułu

Selected qualitative and biochemical parameters of cryopreserved semen of Holstein-Friesian (HF) AI bulls

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Selected qualitative and biochemical parameters were determined in cryopreserved semen used for artificial insemination, sampled from 120 bulls reared at the Animal Breeding and Insemination Center in Bydgoszcz. The total average motility of the analyzed sperm samples was determined at 62.51%. The percentage of motile spermatozoa displaying progressive forward motility was 21.65%. Analyzed samples were characterized by a high percentage of sperm cells with a intact plasma membrane (71.21%) and active mitochondria (71.32%). High efficiency of the enzymatic antioxidant system of the evaluated sperm cells was demonstrated by high activity of CAT, GPx and SOD (494.37, 2847.83 and 5.31U/1x109 spermatozoa, respectively) values and low values of the DNA Fragmentation Index (9.32). The results of the study, obtained with the involvement of advanced analytical methods, indicate a high fertilizing capability of the analyzed sperm samples.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

18

Numer

1

Opis fizyczny

p.237-239,ref.

Twórcy

autor
  • Departament of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland
autor
  • Departament of Animal Genetics, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland
autor
  • Departament of Animal Genetics, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland
autor
  • Departament of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland
autor
  • Departament of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

Bibliografia

  • Fraser L, Lecewicz M, Strzeżek J (2002) Fluorometric assessments of viability and mitochondrial status of boar spermatozoa following liquid storage. Pol J Vet Sci 5: 85-92.
  • Fraser L, Strzeżek J (2005) Effects of freezing-thawing on DNA integrity of boar spermatozoa assessed by the neutral comet assay. Reprod Domest Anim 40: 530-536.
  • Hirano Y, Shibahara H, Obara H, Suzuki T, Takamizawa S, Yamaguchi C Tsunoda H, Sato I (2001) Relationship between Sperm Motility Characteritics Assessed by the Computer-Aided Sperm Analysis (CASA) and fertilization rates in vitro. J Assist Reprod Genet 18: 213-218.
  • Kasimanickam R, Kasimanickam V, Thatcher CD, Nebel RL, Cassell BG (2007) Relationships among lipid peroxidation, glutathione peroxidase, superoxide dismutase, sperm parameters, and competitive index in dairy bulls. Theriogenology 67: 1004-1012.
  • Koziorowska-Gilun M, Gilun P, Fraser L, Koziorowski M, Kordan W, Stefanczyk-Krzymowska S (2013) Antioxidant enzyme activity and mRNA expression in reproductive tract of adult male European bison (Bison bonasus, Linnaeus 1758). Reprod Domest Anim 48: 7-14.
  • Taşdemir U, Buyukleblebici S, Tuncer PB, Coşkun E, Ozgurtas T, Aydyn FN, Buyukleblebici O, Gurcan IS (2013) Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters. Cryobiology 66: 38-42.
  • Thomas CA, Garner DL, DeJarnette JM, Marshall CE (1998) Effect of cryopreservation of bovine sperm organelle function and viability as determined by flow cytometry. Biol Reprod 58: 786-793.
  • Walsh SW, Williams EJ, Evans AC (2011) A review of the causes of poor fertility in high milk producing dairy cows. Anim Reprod Sci 123: 127-138.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-b4035aba-3f48-417a-804a-43faf6c3393e
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