Sodium azide mutagenesis within temporary immersion bioreactors modifies sugarcane in vitro micropropagation rates and aldehyde, chlorophyll, carotenoid, and phenolic profiles

• Autorzy: Gomez D. , Hernandez L. , Martinez J. , Escalante D. , Zevallos B.E. , Yabor L. , Trethowan R. , Beemster G.T.S. , Sershen , Lorenzo J.C.
• Języki publikacji: EN

Abstrakty

EN

Chemical mutagens such as sodium azide (NaN₃) have been widely used to increase genetic variability in crops, but the undirected mutations induced can have undesirable effects, which need to be characterized. This study investigated the effects of in vitro NaN₃ (0–0.45 mM) exposure (30 days) on the micropropagation of sugarcane within temporary immersion bioreactors (TIB). Shoot multiplication rate and cluster fresh weight, and aldehyde, phenolic, carotenoid, and chlorophyll levels were measured on in vitro produced shoots. The soluble phenolic content of the culture medium was also assessed. NaN₃ concentration was negatively correlated with sugarcane shoot multiplication rate and fresh weight; at 0.45 mM NaN₃, these parameters were only 20% and 39% that of the untreated control, respectively. Shoot multiplication rate and fresh weight, and chlorophyll a and b levels were negatively correlated with NaN₃ concentration. In contrast, malondialdehyde, other aldehyde, carotenoid, and exuded phenol levels were positively correlated with NaN₃ concentration. Statistical comparisons suggest that shoot multiplication rate and the biochemical parameters that were positively correlated with NaN₃ concentration may be the most suitable indicators of stress when optimizing the concentration of NaN₃ for sugarcane explants. An interpolated 50% reduction of multiplication rates at 0.23 mM NaN₃ suggests that this concentration to be suitable for TIB-based induction of mutagenesis in shoots and eventual production of agriculturally useful mutants.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2019
• Tom: 41
• Numer: 07
• Opis fizyczny: Article 114 [7p.], fig.,ref.

Twórcy

autor

Gomez D.

• Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba

autor

Hernandez L.

• Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba

autor

Martinez J.

• Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba

autor

Escalante D.

• Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba

autor

Zevallos B.E.

• Escuela Superior Politecnica Agropecuaria de Manabi Manuel Felix Lopez (ESPAMMFL), Campus Politecnico El Limon, Carrera de Ingeniería Agrícola, Calceta, Manabí, Ecuador

autor

Yabor L.

• Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba

autor

Trethowan R.

• Plant Breeding Institute, Faculty of Agriculture and Environment, University of Sydney, Sydney, Australia

autor

Beemster G.T.S.

• Laboratory for Integrated Plant Physiology Research (IMPRES), University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium

autor

Sershen

• School of Life Sciences, University of KwaZulu-Natal, Durban 4001, South Africa

autor

Lorenzo J.C.

• Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba

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Identyfikatory

DOI

10.1007/s11738-019-2911-0