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2019 | 41 | 07 |

Tytuł artykułu

Sodium azide mutagenesis within temporary immersion bioreactors modifies sugarcane in vitro micropropagation rates and aldehyde, chlorophyll, carotenoid, and phenolic profiles

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Chemical mutagens such as sodium azide (NaN₃) have been widely used to increase genetic variability in crops, but the undirected mutations induced can have undesirable effects, which need to be characterized. This study investigated the effects of in vitro NaN₃ (0–0.45 mM) exposure (30 days) on the micropropagation of sugarcane within temporary immersion bioreactors (TIB). Shoot multiplication rate and cluster fresh weight, and aldehyde, phenolic, carotenoid, and chlorophyll levels were measured on in vitro produced shoots. The soluble phenolic content of the culture medium was also assessed. NaN₃ concentration was negatively correlated with sugarcane shoot multiplication rate and fresh weight; at 0.45 mM NaN₃, these parameters were only 20% and 39% that of the untreated control, respectively. Shoot multiplication rate and fresh weight, and chlorophyll a and b levels were negatively correlated with NaN₃ concentration. In contrast, malondialdehyde, other aldehyde, carotenoid, and exuded phenol levels were positively correlated with NaN₃ concentration. Statistical comparisons suggest that shoot multiplication rate and the biochemical parameters that were positively correlated with NaN₃ concentration may be the most suitable indicators of stress when optimizing the concentration of NaN₃ for sugarcane explants. An interpolated 50% reduction of multiplication rates at 0.23 mM NaN₃ suggests that this concentration to be suitable for TIB-based induction of mutagenesis in shoots and eventual production of agriculturally useful mutants.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

41

Numer

07

Opis fizyczny

Article 114 [7p.], fig.,ref.

Twórcy

autor
  • Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba
autor
  • Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba
autor
  • Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba
autor
  • Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba
  • Escuela Superior Politecnica Agropecuaria de Manabi Manuel Felix Lopez (ESPAMMFL), Campus Politecnico El Limon, Carrera de Ingeniería Agrícola, Calceta, Manabí, Ecuador
autor
  • Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba
autor
  • Plant Breeding Institute, Faculty of Agriculture and Environment, University of Sydney, Sydney, Australia
  • Laboratory for Integrated Plant Physiology Research (IMPRES), University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium
autor
  • School of Life Sciences, University of KwaZulu-Natal, Durban 4001, South Africa
autor
  • Laboratory for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Avila, 69450 Ciego de Avila, Cuba

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Bibliografia

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