Small interfering RNA treatment can inhibit Cyprinid herpesvirus 3 associated cell death in vitro

• Autorzy: Adamek M. , Rauch G. , Brogden G. , Steinhagen D.
• Języki publikacji: EN

Abstrakty

EN

A Cyprinid herpesvirus 3 infection of carp induces a disease which causes substantial losses in carp culture. Here we present the use of a possible strategy for the management of the virus infection RNA interference based on small interfering RNAs. As a result of in vitro studies, we found that a mixture of short interfering RNAs specific for viral DNA enzyme synthesis and capsid proteins of the CyHV-3 can be a potential inhibitor of virus replication in fibroblastic cells. This gives the basis for the development of a combinatorial RNA interference strategy to treat CyHV-3 infections.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2014
• Tom: 17
• Numer: 4
• Opis fizyczny: p.733-735,ref.

Twórcy

autor

Adamek M.

• Fish Disease Research Unit, Centre of Infectious Diseases, University of Veterinary Medicine Hannover, Bunteweg 17, D-30559 Hannover, Germany

autor

Rauch G.

• Institute for Evolution and Biodiversity, Westfalische Wilhelms-Universitat Munster, Hufferstrasse 1, D-48149 Munster, Germany

autor

Brogden G.

• Fish Disease Research Unit, Centre of Infectious Diseases, University of Veterinary Medicine Hannover, Bunteweg 17, D-30559 Hannover, Germany

autor

Steinhagen D.

• Fish Disease Research Unit, Centre of Infectious Diseases, University of Veterinary Medicine Hannover, Bunteweg 17, D-30559 Hannover, Germany

Bibliografia

• Adamek M, Rakus KL, Chyb J, Brogden G, Huebner A, Irnazarow I, Steinhagen D (2012) Interferon type I responses to virus infections in carp cells: in vitro studies on Cyprinid herpesvirus 3 and Rhabdovirus carpio infections. Fish Shellfish Immunol 33: 482-493.
• Gotesman M, Soliman H, Besch R, El-Matbouli M (2014) In vitro inhibition of Cyprinid herpesvirus-3 replication by RNAi. J Virol Methods 206: 63-66.
• Lima PC, Harris JO, Cook M (2013) Exploring RNAi as a therapeutic strategy for controlling disease in aquaculture. Fish Shellfish Immunol 34: 729-743.
• Schyth BD, Bramsen JB, Pakula MM, Larashati S, Kjems J, Wengel J, Lorenzen N (2012) In vivo screening of modified siRNAs for non-specific antiviral effect in a small fish model: number and localization in the strands are important. Nucleic Acids Res 40: 4653-4665.

Identyfikatory

DOI

10.2478/pjvs-2014-0108