Role of extracellular matrix and prolactin in functional differentiation of bovine BME-UV1 mammary epithelial cells

• Autorzy: Kozlowski M. , Wilczak J. , Motyl T. , Gajewska M.
• Języki publikacji: EN

Abstrakty

EN

Interactions between extracellular matrix (ECM) and epithelial cells are necessary for proper organisation and function of the epithelium. In the present study we show that bovine mammary epithelial cell line BME-UV1 cultured on ECM components, commercially available as Matrigel™, constitutes a good model for studying mechanisms controlling functional differentiation of the bovine mammary gland. In contact with Matrigel BME-UV1 cells induce apicobasal polarity, and within 16 days form three dimensional (3D) acinar structures with a centrally localized hollow lumen, which structurally resemble mammary alveoli present in the functionally active mammary gland. We have shown that the 3D culture system enables a high expression and proper localisation of integrin receptors and tight junction proteins in BME-UV1 cells to be induced. This effect was not obtained in cells grown in the classical 2D culture system on plastic. Moreover, ECM highly stimulated the synthesis of one of the major milk proteins, β-casein, even in the absence of prolactin. Our results show that contact with ECM plays an important role in the lactogenic activity of bovine MECs, however, prolactin is necessary for the efficient secretion of milk proteins.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2011
• Tom: 14
• Numer: 3
• Opis fizyczny: p.433-442,fig.,ref.

Twórcy

autor

Kozlowski M.

• Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences- SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Wilczak J.

autor

Motyl T.

autor

Gajewska M.

Bibliografia

• Bachelot A, Binart N (2007) Reproductive role of prolactin. Reproduction 133: 361-369.
• Balda MS, Garrett MD, Matter K (2003) The ZO-1-associated Y-box factor ZONAB regulates epithelial cell proliferation and cell density. J Cell Biol 160: 423-432.
• Barcellos-Hoff MH, Aggeler J, Ram TG, Bissell MJ (1989) Functional differentiation and alveolar morphogenesis of primary mammary cultures on reconstituted basement membrane. Development 105: 223-235.
• Blatchford DR, Hendry KA, Wilde CJ (1998) Autocrine regulation of protein secretion in mouse mammary epithelial cells. Biochem Biophys Res Commun 248: 761-766.
• Brisken C, Rajaram RD (2006) Alveolar and lactogenic differentiation. J Mammary Gland Biol Neoplasia 11: 239-248.
• Capuco AV, Ellis S (2005) Bovine mammary progenitor cells: current concepts and future directions. J Mammary Gland Biol Neoplasia 10: 5-15.
• Debnath J, Mills KR, Collins NL, Reginato MJ, Muthuswamy SK, Brugge JS (2002) The role of apoptosis in creating and maintaining luminal space within normal and oncogene-expressing mammary acini. Cell 111: 29-40.
• Debnath J, Muthuswamy SK, Brugge JS (2003) Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures. Methods 30: 256-268.
• Delabarre S, Claudon C, Laurent F (1997) Influence of several extracellular matrix components in primary cultures of bovine mammary epithelial cells. Tissue Cell 29: 99-106.
• Itoh M, Bissell MJ (2003) The organization of tight junctions in epithelia: implications for mammary gland biology and breast tumorigenesis. J Mammary Gland Biol Neoplasia 8: 449-462.
• Kass L, Erler JT, Dembo M, Weaver VM (2007) Mammary epithelial cell: influence of extracellular matrix composition and organization during development and tumorigenesis. Int J Biochem Cell Biol 39: 1987-1994.
• Katz E, Streuli CH (2007) The extracellular matrix as an adhesion checkpoint for mammary epithelial function. Int J Biochem Cell Biol 39: 715-726.
• Kozłowski M, Gajewska M, Majewska A, Jank M, Motyl T (2009) Differences in growth and transcriptomic profile of bovine mammary epithelial monolayer and three-dimensional cell cultures. J Physiol Pharmacol 60 (Suppl 1): 5-14.
• Naylor MJ, Li N, Cheung J, Lowe ET, Lambert E, Marlow R, Wang P, Schatzmann F, Wintermantel T, Schuetz G, Clarke AR, Mueller U, Hynes NE, Streuli CH (2005) Ablation of beta1 integrin in mammary epithelium reveals a key role for integrin in glandular morphogenesis and differentiation. J Cell Biol 171: 717-728.
• Naylor MJ, Lockefeer JA, Horseman ND, Ormandy CJ (2003) Prolactin regulates mammary epithelial cell proliferation via autocrine/paracrine mechanism. Endocrine 20: 111-114.
• Riley LG, Gardiner-Garden M, Thomson PC, Wynn PC, Williamson P, Raadsma HW, Sheehy PA (2010) The influence of extracellular matrix and prolactin on global gene expression profiles of primary bovine mammary epithelial cells in vitro. Anim Genet 41: 55-63.
• Schmidhauser C, Casperson GF, Myers CA, Sanzo KT, Bolten S, Bissell MJ (1992) A novel transcriptional enhancer is involved in the prolactin- and extracellular matrix-dependent regulation of beta-casein gene expression. Mol Biol Cell 3: 699-709.
• Stelwagen K, McFadden HA, Demmer J (1999) Prolactin, alone or in combination with glucocorticoids, enhances tight junction formation and expression of the tight junction protein occludin in mammary cells. Mol Cell Endocrinol 156: 55-61.
• Streuli CH (2003) Cell adhesion In mam mary gland biology and neoplasia. J Mammary Gland Biol Neoplasia 8: 375-381.
• Wall EH, Crawford HM, Ellis SE, Dahl GE, McFadden TB (2006) Mammary response to exogenous prolactin or frequent milking during early lactation in dairy cows. J Dairy Sci 89: 4640-4648.
• Weaver VM, Lelicvre S, Lakins JN, Chrenek MA, Jones JC, Giancotti F, Werb Z, Bissell MJ (2002) Beta4 integrin-dependent formation of polarized three-dimensional architecture confers resistance to apoptosis in normal and malignant mammary epithelium. Cancer Cell 2: 205-216.
• Xian W, Schwertfeger KL, Vargo-Gogola T, Rosen JM (2005) Pleiotropic effects of FGFR1 on cell proliferation, survival, and migration in a 3D mammary epithelial cell model. J Cell Biol 171: 663-673.