Mitochondrial potassium channels in Dictyostelium discoideum

• Autorzy: Laskowski M. , Kicinska A. , Jarmuszkiewicz W. , Szewczyk A.
• Języki publikacji: EN

Abstrakty

EN

The strict control of inner mitochondrial membrane permeability and selective ion transport is essential for mitochondria functioning. Potassium ions homeostasis is an important process for mitochondrial optimal functioning. Currently we characterize mitochondrial potassium channels from one of Dictyostelium species. Preliminary experiments are focused on biophysical and pharmacological characterization of mitochondrial ion channels. Purified inner mitochondrial membranes (submitochondrial particles) were reconstituted into planar lipid bilayer. To form model membranes asolectin from soybean mixture of phospholipids was used. We observed two types of potassium selective ion channels in submitochondrial particle samples: a large- and small-conductance channels. Experiments were performed both in gradient solution 50/150 mM KCl (cis-trans) and in symmetrical solution 150/150 mM KCl at voltages from −50 to 50 mV. Regulation of the channel activity by divalent cations such as Ca2+ and Mg2+ was explored. Additionally, interaction of the ATP with mitochondrial potassium channels was characterized. The knowledge on mitochondrial ion channels may contribute to understanding molecular mechanism of Dictyostelium discoideum functioning.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2012
• Tom: 72
• Numer: 2
• Opis fizyczny: p.198

Twórcy

autor

Laskowski M.

• Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, Warsaw, Poland

autor

Kicinska A.

• Department of Animal Physiology and Development, Adam Mickiewicz University, Poznan, Poland

autor

Jarmuszkiewicz W.

• Department of Animal Physiology and Development, Adam Mickiewicz University, Poznan, Poland

autor

Szewczyk A.

• Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, Warsaw, Poland

Uwagi

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