Epidemiological analysis of Mycobacterium tuberculosis strains isolated from patients of small communities living in the South-East of Poland

• Autorzy: Zaczek A. , Szwaja I. , Skiba M. , Brzostek A. , Puchalski C. , Lewandowska K. , Dziadek J.
• Języki publikacji: EN

Abstrakty

EN

The diversity of Mycobacterium tuberculosis clinical isolates, collected from a single hospital, was analyzed by ligation-mediated PCR techniques: FLiP and FLAP, and hybridization technique, IS6110-RFLP. The isolated strains were divided in terms of location (3 towns of Podkarpackie voivodeship differing in population size) and relationship (8 members of 4 families, each represented by 2 patients). Within each family identical DNA profiles, as well as drug resistance patterns were identified indicating a great chance of transmission of strains within the same family. Identical, or very similar patterns were also shared by strains isolated from unrelated patients living in a very small town (1 200 inhabitants) or hospitalized in the same place and time.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2015
• Tom: 64
• Numer: 3
• Opis fizyczny: p.289-293,fig.,ref.

Twórcy

autor

Zaczek A.

• Faculty of Biology and Agriculture, University of Rzeszow, Rzeszow, Poland

autor

Szwaja I.

• Independent Public Health Care Facility “Sanatorium”, Gorno, Poland

autor

Skiba M.

• Independent Public Health Care Facility “Sanatorium”, Gorno, Poland

autor

Brzostek A.

• Institute of Medical Biology, Polish Academy of Sciences, Lodz, Polamd

autor

Puchalski C.

• Faculty of Biology and Agriculture, University of Rzeszow, Rzeszow, Poland

autor

Lewandowska K.

• Institute of Medical Biology, Polish Academy of Sciences, Lodz, Polamd
• Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, Poland

autor

Dziadek J.

• Faculty of Biology and Agriculture, University of Rzeszow, Rzeszow, Poland
• Institute of Medical Biology, Polish Academy of Sciences, Lodz, Polamd

Bibliografia

• Barnes P.F and M.D. Cave. Molecular epidemiology of tuberculosis. 2003. N. Engl. J. Med. 349: 1149–1156.
• Covan L.S., L. Diem, T. Monson, P. Wand, D. Temporado, T.V. Oemig and J.T. Crawford. 2005. Evaluation of a two-step approach for large-scale, prospective genotyping of Mycobacterium tuberculosis isolates in the United States. J. Clin. Microbiol. 43: 688–695.
• Crawford J.T. 2003. Genotyping in contact investigations: a CDC perspective. Int. J. Tuberc. Lung Dis. 7: 8453–57.
• Janowiec M. 1988. Microbiology and serology (in Polish). National Institute of Medical Publishing.
• Korzeniewska-Koseła M. 2014. Tuberculosis in Poland in 2012 (in Polish). Przegl. Epidemiol. 68: 389–393.
• Kozińska M., Z. Zwolska and E. Augustynowicz-Kopeć. 2011. Transmission of drug-resistant tuberculosis among closely related (in Polish). Postępy Nauk Medycznych 10: 824–830.
• Krawczyk B., A. Samet, J. Leibner, A. Sledzinska and J. Kur. 2006. Evaluation of a PCR melting profile technique for bacterial strain differentiation. J. Clin. Microbiol. 44: 2327–2332.
• Krawczyk B., J. Leibner-Ciszak, K. Stojowska and J. Kur. 2011. The new LM-PCR/shifter method for the genotyping of microorganisms based on the use of a class IIS restriction enzyme and ligation mediated PCR. J. Microbiol. Biotechnol. 21: 1336–1344.
• Kremer K., C. Arnold, A. Cataldi, M.C. Gutiérrez, W.H. Haas, S. Panaiotov, R.A. Skuce, P. Supply, A.G. van der Zanden and D. van Soolingen. 2005. Discriminatory power and reproducibility of novel DNA typing methods for Mycobacterium tuberculosis complex strains. J. Clin. Microbiol. 43: 5628–5638.
• Masny A. and A. Plucienniczak. 2003. Ligation mediated PCR performed at low denaturation temperatures-PCR melting profiles. Nucleic Acids Res. 31(18):e114.
• Moström P., M. Gordon, C. Sola, M. Ridell and N. Rastogi. 2002. Methods used in the molecular epidemiology of tuberculosis. Clin. Microbiol. Infect. 8: 694–704.
• Reisig F., K. Kremer, B. Amthor, D. van Soolingen and W.H. Haas. 2005. Fast ligation-mediated PCR, a fast and reliable method for IS6110-based typing of Mycobacterium tuberculosis complex. J. Clin. Microbiol. 43: 5622–5627.
• Statistical bulletin. 2013. Ministry of Health. http://www.csioz.gov.pl/publikacja.php?id=6, 1.08.2015.
• Supply P., C. Allix, S. Lesjean, M. Cardoso-Oelemann, S. Rusch-Gerdes, E. Willery and others. 2006. Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of M. tuberculosis. J. Clin. Microbiol. 44: 4498–4510.
• Valcheva V., I. Mokrousov, N. Rastogi, O. Narvskaya and N. Markova. 2008. Molecular characterization of Mycobacterium tuberculosis isolates from different regions of Bulgaria. J. Clin. Microbiol. 46: 1014–1018.
• van Embden J.D., M.D. Cave, J.T. Crawford, J.W. Dale, K.D. Eisenach, B. Gicquel, P. Hermans, C. Martin, R. McAdam and T.M. Shinnick. 1993. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J. Clin. Microbiol. 31: 406–409.
• World Health Organization, Global Tuberculosis Report. 2012. Zaczek A., A. Brzostek, A. Gorna, A. Sajduda, A. Wojtasik and J. Dziadek. 2013a. Application of FLiP method for differentiation of Mycobacterium tuberculosis strains in comparison to commonly used methods, spoligotyping and MIRU-VNTR typing. Pol. J. Microbiol. 62: 73–76.
• Zaczek A., M. Ziolkiewicz, A. Wojtasik, J. Dziadek and A. Sajduda. 2013b. IS6110-based differentiation of Mycobacterium tuberculosis strains. Pol. J. Microbiol. 62: 201–204.
• Zaczek A., A. Brzostek, A. Wojtasik, J. Dziadek and A. Sajduda. 2013c. Genotyping of clinical Mycobacterium tuberculosis isolates based on IS6110 and MIRU-VNTR polymorphisms. Biomed. Res. Int. 2013: 865197.
• Zaczek A., A. Brzostek, A. Kuroń, A. Wojtasik, A. Sajduda and J. Dziadek. 2014a. Development of a new ligation-mediated PCR method for the differentiation of Mycobacterium tuberculosis strains. Int. J. Tuberc. Lung Dis. 18: 302–309.
• Zaczek A., A. Brzostek, A. Wojtasik, A. Sajduda and J. Dziadek. 2014b. Comparison of ligation-mediated PCR methods in differentiation of Mycobacterium tuberculosis strains. Biomed. Res. Int. 2014: 782071.
• Zozio T., C. Allix, S. Gunal, Z. Saribas, A. Alp, R. Durmaz, M. Fauville-Dufaux, N. Rastogi and C. Sola. 2005. Genotyping of Mycobacterium tuberculosis clinical isolates in two cities of Turkey: description of a new family of genotypes that is phylogeographically specific for Asia Minor. BMC Microbiol. 5: 44.