Specific binding properties of 125I-apamin in various structures of the rat central nervous system

• Autorzy: Janicki P.K.
• Języki publikacji: EN

Abstrakty

EN

Janicki, P. K.: Specific binding properties of ¹²⁵ I-apamin to various structures of the rat central nervous system. Acta physiol, pol., 1989, 40 (2): 235-239. The properties of ¹²⁵ I-apamin binding with raft ceaxtrail nervous system slices were analysed in vitro using computerized densitometric autoradiography. Scatchard analysis performed for the data of binding experiments in rat brain and spinal cord demonstrates that apamin binds to a single class of non-interacting binding sites in all investigated structures. The dissociation constant values (KD) weTe similar in all investigated structures (31-38 pM). The maximal binding capacity (Bmax) was observed in the structures of limbic olfactory system (30 fmol/mg protein), the lowest in brain white matter (0.5 fmol/mg protein). It is concluded that the observed pattern of ¹²⁵ I-apamin binding might represent the topography of a class of Ca²⁺ dependent K⁺ channels in the rat central nervous system.

• Wydawca: -
• Czasopismo: Acta Physiologica Polonica
• Rocznik: 1989
• Tom: 40
• Numer: 2
• Opis fizyczny: p.235-239,fig.,ref.

Twórcy

autor

Janicki P.K.

• Department of Pharmacology, Institute of Physiological Sciences, School of Medicine, Warsaw, Poland

Bibliografia

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