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2017 | 77 | Suppl.1 |
Tytuł artykułu

Impact of environmental enrichment on anxiety and learning in the rat model of epilepsy induced by electrical stimulation of the amygdala

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
INTRODUCTION: Environment plays influential role in the development of many brain disorders, however its role in modulation of epilepsy phenotype has not been studied in details. AIM(S): The aim of this study was to investigate whether environmental enrichment impacts anxiety and learning in experimental model of epilepsy. METHOD(S): Male Sprague-Dawley rats were allocated to either environmentally enriched (EE; n=13) or standard housing condition (SH; n=13). Epilepsy was induced by SE (Status epilepticus) evoked by electrical stimulation of the amygdala (25 min, 100 ms train of 1 ms, 60 Hz bipolar pulses, 400 μA, every 0.5 s). Following tests were conducted to assess the behavior of animals: behavioral hyperexcitability, open field, new object recognition, elevated plus maze, social interactions, and Morris water maze. Blood was withdrawn on days 7 and 29 after stimulation and on the day of perfusion to assess cortisol levels. RESULTS: Environmental enrichment significantly reduced anxiety levels. We observed, reduced mobility in the open field test (EE=2.6±3.3; SH=179.1±107.8 s; p<0,0001), decrease in total distance travelled in the social interactions test (EE=1210.2±574.4; SH=2937.0±711.3 cm; p<0,0001) or decreased touch-response test in the behavioral hyperexcitability test (score: EE=2.1±1.1; SH=3.6±1.8; p<0,0001). SH animals showed impaired spatial memory and learning compared to EE animals. Rats from EE group spent more time near platform (EE=25.5±4.7; SH=21.5±5.0 s; p<0,05) in Morris Water Maze test. Moreover, SH rats showed hyperactivity and thigmotaxis. Blood analysis demonstrated that SH rats had significantly higher level of cortisol (EE=0.4±0.7; SH=1.1±0.6 µg/dl; p<0,01) compared to EE rats. CONCLUSIONS: The present study indicates that environmental enrichment had beneficial effects on anxiety and learning and memory, which may be caused by lower stress hormone levels. FINANCIAL SUPPORT: This work was supported by the FP7-HEALTH project 602102 (EPITARGET) and Polish Ministry of Science and Education grant W19/7. PR/2014.
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-
Rocznik
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77
Numer
Opis fizyczny
p.119
Twórcy
autor
  • Department of Molecular and Cellular Neurobiology, Institute of Experimental Biology, Warsaw, Poland
autor
  • Department of Molecular and Cellular Neurobiology, Institute of Experimental Biology, Warsaw, Poland
autor
  • Department of Molecular and Cellular Neurobiology, Institute of Experimental Biology, Warsaw, Poland
  • Department of Molecular and Cellular Neurobiology, Institute of Experimental Biology, Warsaw, Poland
Bibliografia
Typ dokumentu
Bibliografia
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bwmeta1.element.agro-b06196d7-16c7-47df-b6ea-3a23a41b10df
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