Characterization of drug resistance-associated TevAT1 gene of Trypanosoma evansi from Philippine water buffaloes (Bubalus bubalis)

• Autorzy: Mingala C.N. , Pasag A.C.P. , Salinas M.B.S. , Balbin M.M. , Villanueva M.A.
• Języki publikacji: EN

Abstrakty

EN

This study detected and characterized the TevAT1 gene of Trypanosoma evansi isolates from Philippine water buffaloes (Bubalus bubalis). A total of 68 blood samples from Philippine water buffaloes were subjected to DNA extraction and PCR assay was performed using RoTat 1.2 gene to detect T. evansi. Those samples positive for T. evansi subsequently underwent another PCR assay to detect the presence of TevAT1 gene. Trypanosoma evansi was detected in 26.47% (18/68) blood samples in which distributed throughout the main islands of the country (4 from Luzon, 2 from Visayas and 12 from Mindanao). However, only 10 of these samples were positive for TevAT1 gene. Sequence alignment of the TevAT1 gene from local isolates showed no single nucleotide polymorphisms when compared to other strains in various countries. Those T. evansi without the gene of interest could be possibly resistant to some trypanocidal drugs but this needs to be further investigated in-vitro or in-vivo.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2019
• Tom: 65
• Numer: 4
• Opis fizyczny: p.381–386,fig.,ref.

Twórcy

autor

Mingala C.N.

• College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines
• Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz 3120, Nueva Ecija, Philippines
• Department of Animal Science, College of Agriculture, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines

autor

Pasag A.C.P.

• College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines

autor

Salinas M.B.S.

• College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines

autor

Balbin M.M.

• Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz 3120, Nueva Ecija, Philippines

autor

Villanueva M.A.

• College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines
• Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz 3120, Nueva Ecija, Philippines

Bibliografia

• [1] Villareal M.V., Mingala, C.N., Rivera W.L. 2013. Molecular characterization of Trypanosoma evansi isolates from water buffaloes (Bubalus bubalis) in the Philippines. Acta Parasitologica 58: 6-12. https://doi.org/10.2478/s11686-013-0110-5
• [2] Manuel M.F. 1998. Sporadic outbreaks of surra in the Philippines and its economic impact. Journal of Protozoology Research 8: 131-138.
• [3] Baticados W.N., Castro D.L., Baticados A.M. 2011. Parasitological and PCR detection of Trypanosoma evansi in buffaloes from Luzon, Philippines. Ceylon Journal of Science 40: 141-146. https://doi.org/10.4038/cjsbs.v40i2.3930
• [4] Macaraeg B.B., Lazaro J.V., Abes N.S., Mingala C.N. 2013. In-vivo assessment of the effects of trypanocidal drugs against Trypanosoma evansi isolates from Philippine water buffaloes (Bubalus bubalis). Veterinarski Arhiv 83: 381-392.
• [5] Ventura R.M., Takeda G.F, Silva R.A., Nunes V.L., Buck G.A., Teixeria M.M.G. 2002. Genetic relatedness among Trypanosoma evansi stocks by random amplification of polymorphic DNA and evaluation of a synapomorphic DNA fragment for species specific diagnosis. International Journal of Parasitology 32: 53-63.
• [6] Pruvot M., Kamyingkird K., Desquesnes M., Sarataphan N., Jittapalapong S. 2010. A comparison of six primer sets for the detection of Trypanosoma evansi by polymerase chain reaction in rodents and Thai livestock. Veterinary Parasitology 171: 185-193.https://doi.org/10.1016/j.vetpar.2010.04.001
• [7] Claes F., Radwanska M., Urakawa T., Majiwa P.A., Goddeeris B., Buscher P. 2004. Variable surface glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections. Kinetoplastid Biology and Disease 3: 1-3. https://doi.org/10.1186/1475-9292-3-3
• [8] Delespaux V., De Koning H. 2007. Drugs and drug resistance in African trypanosomiasis. Drug Resistance Updates 10: 30-50. https://doi.org/10.1016/j.drup.2007.02.004
• [9] Tsegaye B., Dagnachew S., Terefe G. 2015. Review on drug resistant animal Trypanosomes in Africa and overseas. African Journal of Basic and Applied Sciences 7: 78-83. doi:10.5829/idosi.ajbas.2015.7.2.9370
• [10] Zhang Z.Q., Giroud C., Baltz T. 1993. Trypanosoma evansi: in vivo and in vitro determination of trypanocide resistance profiles. Experimental Parasitology 77: 387-394. https://doi.org/10.1006/expr.1993.1098
• [11] Anene B.M., Onah D.N., Nawa Y. 2001. Drug resistance in pathogenic african Trypanosomes: What hopes for the future? Veterinary Parasitology 96: 83-100. https://doi.org/10.1016/s0304-4017(00)00427-1
• [12] Melaku A., Birasa B. 2013. Drugs and drug resistance in African animal trypanosomiasis: a review. European Journal of Biological Sciences 5: 82-89. doi:10.5829/idosi.ejas.2013.5.3.75164
• [13] Moti Y., De Deken R., Thys E., Van Den Abbeele J., Duchateau L., Delespaux V. 2015. PCR and microsatellite analysis of diminazene aceturate resistance of bovine trypanosomes correlated to knowledge, attitude and practice of livestock keepers in South-Western Ethopia. Acta Tropica 146: 45-52. https://doi.org/10.1016/j.actatropica.2015.02.015
• [14] Matovu E., Geiser F., Schneider V., Mäser P., Enyaru J.C., Kaminsky R., Gallati S., Seebeck T. 2001. Genetic variants of the TbAT 1 adenosine transporter from African trypanosomes in relapse infections following melarsopol therapy. Molecular and Biochemical Parasitology 117: 73-81. https://doi.org/10.1016/s0166-6851(01)00332-2
• [15] Suswam E.A., Taylor D.W., Ross C.A., Martin R.J. 2001. Changes in properties of adenosine transporters in Trypanosoma evansi and modes of selection of resistance to the melaminophenyl arsenical drug, Mel Cy. Veterinary Parasitology 102: 193-208. https://doi.org/10.1016/s0304-4017(01)00533-7
• [16] Witola W.H., Inoue N., Ohashi K., Onuma M. 2004. RNA-interference silencing of the adenosine transporter-1 gene in Trypanosoma evansi confers resistance to diminazene aceturate. Experimental Parasitology 107: 47-57. https://doi.org/10.1016/ j.exppara.2004.03.013
• [17] Witola W.H., Tsuda A., Inoue N., Ohashi K., Onuma M. 2005. Acquired resistance to berenil in a cloned isolate of Trypanosoma evansi is associated with upregulation of a novel gene, TeDR40. Parasitology 131(Pt 5): 635-646. https://doi.org/10.1017/S003118200500836X
• [18] Medina N.P., Mingala C.N. 2016. Transporter protein and drug resistance of Trypanosoma. Animal of Parasitology 62: 11-15. https://doi.org/10.17420/ap6201.26
• [19] Suswam E.A., Ross C.A., Martin R.J. 2003. Changes in adenosine transport associated with melaminophenyl arsenical (Mel CY) resistance in Trypanosoma evansi: down-regulation and affinity changes of the P2 transporter. Parasitology 127(Pt 6): 543-549.https://doi.org/10.1017/s003118200300413x
• [20] Mäser P., Sütterlin C., Kralli A., Kaminsky R. 1999. A nucleoside transporter from Trypanosoma brucei involved in drug resistance. Science 285(5425): 242-244. https://doi.org/10.1126/science.285.5425.242
• [21] Munday J.C., Eze A.A., Baker N., Glover L., Clucas C., Aguinaga Andrés D., Natto M.J., Teka I.A., McDonald J., Lee R.S., Graf F.E., Ludin P., Burchmore R.J., Turner C.M., Tait A., MacLeod A., Mäser P., Barrett M.P., Horn D., De Koning H.P. 2014. Trypanosoma brucei aquaglyceroporin 2 is a high-affinity transporter for pentamidine and melaminophenyl arsenic drugs and the main genetic determinant of resistance to these drugs. Journal of Antimicrobial Chemotherapy 69: 651-663. doi:10.1093/jac/dkt442
• [22] Stewart M.L., Burchmore R.J., Clucas C., Hertz-Fowler C., Brooks K., Tait A., Macleod A., Turner C.M., De Koning H.P., Wong P.E., Barrett M.P. 2010. Multiple genetic mechanism lead to loss of functional TbAT1 expression in drug-resistant trypanosomes. Eukaryotic Cell 9: 336-343. https://doi.org/10.1128/EC.00200-09

Identyfikatory

DOI

10.17420/ap6504.224