Phylogenetic analysis based on 16S rRNA gene of a thermophilic protease-secreting Bacillus gelatini-TPNK-3 isolate from Kiteezi Landfill, Uganda

• Autorzy: Omara J. , Ekeya T. P. , Hawumba J. F.
• Języki publikacji: EN

Abstrakty

EN

A thermophilic protease-secreting bacterial isolate, TPNK-3, from Kiteezi landfill, is an aerobic Gram-positive spore-forming bacterium with rod-shaped cells (3.28 μm long and 0.45 μm wide). Optimal growth was observed at 55°C and pH of 7.0, and the isolate tolerates up to 5% (w/v) NaCl, exhibits extracellular amylolytic, cellulolytic and caseinolytic activities, utilizes a range of carbon and nitrogen sources and has a GC content of 45 mol%. The 16S rRNA gene sequence analysis showed that the studied bacterium belongs to the genus Bacillus, and closest to Bacillus gelatini (> 99.9%). Consequently, isolate TPNK3 is tentatively described as Bacillus gelatini strain TPNK3.

Słowa kluczowe

EN

phylogenetic analysis; 16S rRNA gene; thermophilic protease; bacteria; Bacillus gelatini; isolate; Kiteezi Landfill; Uganda

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2012
• Tom: 61
• Numer: 3
• Opis fizyczny: p.227-231,fig.,ref.

Twórcy

autor

Omara J.

• Department of Biochemistry, Makerere University, P.O.Box 7062, Kampala, Uganda

autor

Ekeya T. P.

autor

Hawumba J. F.

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