Endonuclease restriction of SCAR amplicons SC811 is required to identify Ns-false-positive markers in PVS-susceptible potato cultivars

• Autorzy: Szajko K , Chrzanowska M , Strzelczyk-Zyta D , Zagorska H , Marczewski W
• Języki publikacji: EN

Abstrakty

EN

The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811₄₅₄ revealed additional amplicons with a similar electrophoretic migration rate as that of SC81₄₅₄, which resulted in ambiguous determination of the genotype at the Ns locus. MboI or FokI treatment of the PCR products allowed to detect their Ns-unspecificity in PVS-susceptible potato cultivars.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2008
• Tom: 49
• Numer: 1
• Opis fizyczny: p.45-47,fig.,ref.

Twórcy

autor

Szajko K

• Plant Breeding and Acclimatization Institute, Platanowa 19, 05831 Mlochow, Poland

autor

Chrzanowska M

• Plant Breeding and Acclimatization Institute, Platanowa 19, 05831 Mlochow, Poland

autor

Strzelczyk-Zyta D

• Plant Breeding and Acclimatization Institute, Platanowa 19, 05831 Mlochow, Poland

autor

Zagorska H

• Plant Breeding and Acclimatization Institute, Platanowa 19, 05831 Mlochow, Poland

autor

Marczewski W

• Plant Breeding and Acclimatization Institute, Platanowa 19, 05831 Mlochow, Poland

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