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2007 | 47 | 2 |

Tytuł artykułu

Transmission, ELISA and SDS-PAGE results of some Maize streak virus isolates from different parts of Nigeria

Warianty tytułu

PL
Przenoszenie sie oraz wyniki testow ELISA i SDS-PAGE wybranych izolatow Maize streak virus pochodzacych z roznych regionow Nigerii

Języki publikacji

EN

Abstrakty

EN
Field surveys were undertaken in 1997-1999 across five ecological zones in Nigeria to collect isolates of Maize streak virus (MSV), genus Mastrevirus. Apart from maize (Zea mays L.), 15 other grass species were found with MSV symptoms in Nigeria. These hosts showed two types of symptoms viz: mild (with or without mottle) or severe (typical symptoms in maize). When Cicadulina storeyi China was used to attempt transmission of these isolates of MSV to seedlings a susceptible maize hybrid CML 254 X CML 247, six isolates were not transmissible to maize. Seven isolates that were transmissible to maize produced mild symptoms. The viral agents causing typical or severe streak symptoms in Axonopus compressus (Sw.) P. Beauv., Brachiaria distichophylla (Tm.) Stapf, Dactyloctenium aegyptium (Linn.) P. Beauv. and Setaria barbata (Lam.) Kunth produced symptoms that were typical of MSV in farmers fields, when transmitted to maize. Out of 33 plant species that seedlings were challenged with MSV, only eight proved susceptible. Four of them showed mild symptoms while the other four showed severe symptoms of MSV. Only three isolates collected during the surveys did not react with a MSV polyclonal antiserum produced in mice in Double Antibody Sandwich-Enzyme-Linked Immunosorbent Assay (DAS-ELISA). These isolates were found in Andropogon gayanus Kunth (from Ka- duna), Thelepogon elegans Roth ex Toem & Schult (from Kadawa) and Rottboellia cochinchinensis (Lour.) Clayton (from Jos) exhibited mild streak/mottle symptoms. Specific monoclonal antibodies, raised against MSV, reacted with 12 out of 25 samples tested. The DAS-ELISA data also showed significant variation in concentration of the virus in the different plant hosts. The relationship dendogram through SDS-PAGE among eight purified virus isolates show 55-90% variation. At 0.55 coefficient of similarity, the dendogram divided the samples into two groups while at 0.9 coefficient of similarity, the 8 isolates were identified as distinct genetic entities.
PL
W latach 1997-1999 podjęto badania w pięciu strefach ekologicznych Nigerii w celu zebrania izolatów Maize streak virus z rodzaju Mastervirus. Oprócz kukurydzy (Zea mays L.) stwierdzono 15 innych gatunków traw z symptomami infekcji MSV. Badane rośliny żywicielskie miały dwa rodzaje objawów, mianowicie łagodne (z plamami lub bez) albo ostre (typowe objawy, jak w przypadku kukurydzy). Kiedy w próbie przeniesienia izolatów MSV na sadzonki podatnej hybrydy kukurydzy CML 254 X CML 247 użyto Cicadulina storeyi China, sześć z nich nie przeniosło się. Siedem izolatów, które się przenosiły spowodowało wystąpienie łagodnych objawów. Czynniki wirusowe powodujące typowe lub ostre objawy na Axonopus compressus (Sw.) P. Beauv., Brachiaria distichophylla (Tm.) Stapf, Dadyloctenium aegyptinum (Linn.) P. Beauv. i Setaria barbata (Lam.) Kunth spowodowały wystąpienie objawów typowych dla infekcji MSV na polach farmerów kukurydzy, na które zawleczono wirusa. Spośród 33 gatunków roślin, których sadzonki infekowano MSV, tylko osiem było podatnych. Cztery z nich miały łagodne objawy, natomiast cztery pozostałe ostre. Jedynie trzy uzyskane izolaty nie reagowały podczas testu DAS-ELISA (Double Antibody Sandwich - Enzyme Linked Immunosorbent Assay) z poliklonalnym antyserum wyhodowanym na myszach. Izolaty te zebrano z Andropogon gayanus Kunth (z Kaduny), Thelepogon elegans Roth ex Toem & Schult (z Kadawy) i Rottboellia cochinchinensis (Lour.) Clayton (z Jos); rośliny wykazywały łagodne objawy (pasemka/plamki). Zastosowane specyficzne przeciwciała monoklonalne skierowane przeciwko MSV reagowały z 12 próbkami z 25 badanych. Dane DAS-ELISA wykazały również znaczne zróżnicowanie stężeń wirusa w różnych roślinach żywicielskich. Drzewo pokrewieństwa wynikające z SDS-PAGE pomiędzy ośmioma oczyszczonymi izolatami wirusa wykazało 55-90% zróżnicowania. Przy współczynniku prawdopodobieństwa 0,55 dendrogram dzieli próbki na dwie grupy, natomiast przy współczynniku 0,9 osiem powyższych izolatów stanowiło odrębne jednostki genetyczne.

Wydawca

-

Rocznik

Tom

47

Numer

2

Opis fizyczny

p.197-212,fig.,ref.

Twórcy

autor
  • International Institute of Tropical Agriculture, Oyo Road, P.M.B.5320, Ibadan, Nigeria

Bibliografia

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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