Studies on the translation mechanism of subgenomic RNA of potato leafroll virus

• Autorzy: Juszczuk M. , Zagorski-Ostoja W. , Hulanicka D.M.
• Języki publikacji: EN

Abstrakty

EN

The expression of open reading frames located on the subgenomic RNA (sgRNA) has been studied in an in vitro transcription and translation system. The obtained results indicate: a) translation of sgRNA occurs according to the scanning model since the insertion of a palindrome (AGo = -61 kcal/mol) pre­vents the initiation of translation; b) ORF6 is translated by suppression of the stop codon separating ORF4 from ORF6 and the presence of suppressor tRNA is necessary for the read through; c) the presence of leader sequence of sgRNA (212 nucleotides) decreases the translation efficiency of ORFs located down­stream and it afTects the ratio of products of ORF4 and ORF5; d) 3UTR does not influence on an expression of genes located on the sgRNA.

Słowa kluczowe

EN

RNA; transcription system; translation mechanism; infection; tRNA; potato leafroll virus; in vitro; genetic information

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1997
• Tom: 44
• Numer: 1
• Opis fizyczny: p.69-77,fig.

Twórcy

autor

Juszczuk M.

• Polish Academy of Sciences, A.Pawinskiego 5a, 02-106 Warsaw, Poland

autor

Zagorski-Ostoja W.

autor

Hulanicka D.M.

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