Larval toxocarosis in mice: immunoreactivity after multiple high dose infections

• Autorzy: Aldawek A M , Levkut M , Revajova V , Dvoroznakova E
• Języki publikacji: EN

Abstrakty

EN

Immunological responses of mice to threefold infection with infective Toxocara canis were studied up to 42 days. Mice of the experimental group were infected orally with 1000 infective eggs on days 0, 12, and 20 of the experiment (a total of 3000 eggs/mouse). The proliferative activity of spleen lymphocytes, production of specific antibodies in the serum, leukocyte counts, and T cells subpopulations in the blood and spleens were examined. The spleen T cells were significantly inhibited to 21 day post infection (dpi) while B cells were stimulated. An increased level of specific antibodies persisted almost the whole experiment. Leukocytosis with a dominant lymphocytosis was evident. In the peripheral blood the number of both CD8⁺ and CD4⁺ cells was significantly increased. Contradictory, a significant increase of the spleen CD8⁺ cells with a significant decrease of CD4⁺ cells was observed. In conclusion, the multiple high dose infection resulted in variable changes in the immune effector mechanisms. Some components were stimulated while others were inhibited which may suggest that immunosuppression is not the only major outcome of larval toxocarosis.

Słowa kluczowe

EN

infection; mice; serum; cellular immune response; blood; immunoreactivity; T cell; antibody; reinfection; humoral immune response; mouse; toxocariasis; spleen; leucocyte; Toxocara canis

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2002
• Tom: 47
• Numer: 3
• Opis fizyczny: p.249-254,fig.

Twórcy

autor

Aldawek A M

• University of Veterinary Medicine, Komenskeho 73, 041 81 Kosice, Slovak Republic
• Faculty of Veterinary Medicine, Al-Fateh University, Tripoli, Libya

autor

Levkut M

• University of Veterinary Medicine, Komenskeho 73, 041 81 Kosice, Slovak Republic

autor

Revajova V

• University of Veterinary Medicine, Komenskeho 73, 041 81 Kosice, Slovak Republic

autor

Dvoroznakova E

• Parasitological Institute SAS, Hlinkova 3, 040 01 Kosice, Slovak Republic

Bibliografia

• Aldawek A.M., Levkut M., Revajová V., Dvorožňáková E., Dubinský P. 2001. Morphological changes in mice after multiple infection with Toxocara canis. Folia Veterinaria, 45, 31-34.
• Borošková Z., Dvorožňáková E., Tomašovičová O. 1999. Cellular and humoral immune response in mice with long-term Toxocara canis reinfection. Helminthologia, 36, 13-18.
• Borošková Z., Šoltýs J., Havasiová-Reiterová K., Dubinský P., Tomašovičová O., Turčeková L. 1993. Response of lymphocytes to mitogens and the antibody level in mice with experimental larval toxocarosis. Helminthologia, 30, 115-118.
• Bowman D.D., Mika-Grieve M., Grieve R.P. 1987. Circulating excretory-secretory antigen level and specific IgG and IgM responses in mice infected with Toxocara canis. American Journal of Tropical Medicine and Hygiene, 36, 75-82.
• Dvorožňáková E., Borošková Z., Dubinský P., Tomašovičová O., Machnicka B. 2000. Toxocara canis in mice: Immune responses after infection and immunization. Helminthologia, 37, 199-204.
• Freire-Garabal M., Belmonte A., Orallo F., Couceiro J., Nunez M.J. 1991. Effects of alprazolam on T-cell immunosuppressive response to surgical stress in mice. Cancer Letters, 58, 183- 187.
• Glickman L.T., Grieve R.B., De Gregory K.T., Friedman H.S., Stockman J. A. 1984. Toxocara antigen and mitogen-induced lymphocyte blastogenesis for diagnosis of visceral larva migrans: Clinical and experimental findings in macaques and human beings. American Journal of Veterinary Research, 45, 1235-1239.
• Gupta A.K., Ayachit V.L., Lad V.J., Rodriguez J.J. 1990. Antibody response to lipopolysaccharide in experimental canine toxocariasis of mice. Indian Journal of Parasitology, 14, 63-65.
• Havasiová-Reiterová K., Tomašovičová O., Dubinský P. 1995. Effect of various doses of infective Toxocara canis eggs on the humoral response and larval distribution in mice. Parasitology Research, 81, 13-17.
• Hudson K.M., Byner C., Freeman J.C., Terry R.J. 1976. Immunosuppression, high IgM and evasion of the immune response in murine trypanosomiasis. Nature (Lond.), 264, 256-258.
• Kayes S.G. 1984. Spleen cell responses in experimental murine toxocariasis. Journal of Parasitology, 70, 522-529.
• Kayes S.G. 1997. Human toxocariasis and the visceral larva migrans syndrome: correlative immunopathology. In: Immunopathogenetic aspects of disease induced by helminth parasites (Ed. D.O. Freedman). Chemical Immunology, Karger, Basel, 66, 92-124.
• Kayes S.G., Omholt P.E., Grieve R.B. 1985. Immune responses of CBA/J mice to graded infections with Toxocara canis. Infection and Immunity, 48, 697-703.
• Komatsu T., Yoshimoto T., Okamura H., Yamatodani A., Saeki N., Shinka S. 1996. Immunological parameters affected by larval Ascaris suum infection. Japanese Journal of Parasitology, 45, 31-42.
• Levkut M., Revajová V., Dvorožňáková E., Reiterová K., Dubinský P., Krupicer I., Moncol D.J. 1999. Effect of Ascaris suum reinfection on immunoreactivity in lambs. Helminthologia, 36, 69-74.
• Lloyd S. 1987. Immunology and visceral larval migrans. In: Immune responses in parasitic infections. Vol. 1 (Ed. E.J.L. Souisby). CRC, Boca Raton, Florida, 299-324.
• Lloyd S., Amerasinghe P.H., Souisby E.J.L. 1983. Periparturient immunosuppression in bitch and its influence on infection with Toxocara canis. Journal of Small Animal Practice, 24, 237-239.
• Munck A., Guyre P.M., Holbrook N.J. 1984. Physiological functions of glucocorticoids in stress and their relation to pharmacological actions. Endocrine Reviews, 5, 25 - 44.
• Pagé M., Bejaouni N., Ciqo-Mars B., Lemieux P. 1988. Optimization of tetrazolium based colorimetric assay for the measurement of cell number and cytotoxicity. International Journal of Immunopharmacology, 10, 785-793.
• Smith H.V. 1993. Antibody reactivity in human toxocariosis. In: Toxocara and toxocariosis (Eds. J.W. Lewis and R.M. Maizels). British Society for Parasitology, Institute of Biology, London, 91-109.
• Stein M., Keller S.E., Schleifer S.J. 1985. Stress and immunomodulation: The role of depression and neuroendocrine function. Journal of Immunology, 135, 827-833.
• Šoltýs J., Borošková Z., Dubinský P., Tomašovičová O., Auer H,, Aspöck H. 1996. Effect of glucan immunomodulator on the immune response and larval burdens in mice with experimental toxocarosis. Applied Parasitology, 37, 161-167.
• Tizard I.R. 2000. Veterinary immunology: An introduction. W.B. Saunders Company, USA.
• Yamashita U., Jiang H., Inoue H., Mutoh Y., Furukawa T. 1993. Immune functions to Toxocara canis-infected mice. Japanese Journal of Parasitology, 42, 211-219.