Wirus choroby niebieskiego jezyka - budowa i replikacja

• Autorzy: Niedbalski W , Kesy A.

Warianty tytułu

EN

Bluetongue virus [BTV]: structure and replication

• Języki publikacji: PL

Abstrakty

EN

The article reviews the structure of BTV and the role of capsid proteins in the replication process. The BTV is an icosahedral virus with a genome of approximately 19 200 base pairs and composed of ten linear segments of double-stranded RNA (dsRNA). These RNA segments are packaged in a triple layered protein capsid (88 nm in diameter). The outer shell of capsid is composed of two structural proteins: 60 trimers of VP2, which acting as a hemagglutinin (HA) is responsible for glycoprotein receptor binding, and 120 trimers of VP5 that mediate the release of viral particles from endosomal compartments into cytoplasm and undergo pH dependent conformational changes that allow membrane fusion and syncytium formation. The intermediate layer consists of VP7 - protein the major immunodominant of BTV. This layer surrounds the subcore (54 nm) which consist of 12 decamers of the VP3 protein (role in the structural integrity of the virus core) and three minor structural proteins: VP1 (replicase), VP4 (mRNA capping enzyme) and VP6 (RNA-dependent ATPase and helicase). Moreover, the viral subcore consists of nonstructural proteins: NS1, NS2, NS3 and NS3A, which participate in the control of BTV replication, maturation and export from infected cell.

Słowa kluczowe

PL

wirusologia; czynniki chorobotworcze; wirusy; wirus choroby niebieskiego jezyka; budowa; bialka wirusowe; bialka strukturalne; bialka niestrukturalne; replikacja wirusow

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2009
• Tom: 65
• Numer: 09
• Opis fizyczny: s.597-600,rys.,tab.,bibliogr.

Twórcy

autor

Niedbalski W

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy w Pulawach, Zaklad Pryszczycy, ul.Wodna 7, 98-220 Zdunska Wola

autor

Kesy A.

Bibliografia

• 1.Anthony S., Jonem H., Darpel K. E., Elliott H., Maan S., Samuel A., Mellor P. S., Mertens P. P.: A duplex RT-PCR assay for detection of genome segment 7 (VP7 gene) from 24 BTV serotypes. J. Virol. Methods 2007, 141, 188-197.
• 2.Beaton A., Rodriguez J., Redy Y. K., Roy P.: The membrane trafficking protein calpactin forms a complex with bluetongue wirus protein NS3 and mediates wirus relase. Proc. Natl. Acad. Sci. 2002, 99, 13154-13159.
• 3.Bhattacharaya B., Noad R. J., Roy P.: Interaction between bluetongue virus outer capsid protein VP2 and vimentin is necessary for virus egress. Virol. J. 2007, 4, 7.
• 4.Boyce M., Wehrfritz J., Noad R., Roy P.: Purified recombinant bluetongue wirus VP1 exhibits RNA replicase activity. J. Virol. 2004, 78, 3994-4002.
• 5.Forzan M., Marsh M., Roy P.: Bluetongue virus entry into the cells. J. Virol. 2007, 81, 4819-4827.
• 6.Forzan M., Wirblich C., Roy P.: A capsid protein of nonenveloped bluetongue virus exhibits membrane fusion activity. Proc. Natl. Acad. Sci. USA 2004, 101, 2100-2105.
• 7.Grimes J. M., Burroughs J. N., Gouet P., Diprose J. M., Malby R., Zientara S.: The atomic structure of the bluetongue virus core. Nature 1998, 395, 470-478.
• 8.Han Z., Harty R. N.: The NS3 protein of bluetongue virus exhibits viroporin-like properties. J. Biol. Chem. 2004, 279, 43092-43097.
• 9.Hassan S. S., Roy P.: Expression and functional characterization of bluetongue virus VP2 protein: role in cell entry. J. Virol. 1999, 73, 9832-9842.
• 10.Hassan S. H., Wirblich C., Forzan M., Roy P.: Expression and functional characterization of bluetongue virus VP5 protein: role in cellular permeabilization. J. Virol. 2001, 75, 8356-8367.
• 11.Horscroft N. J., Roy P.: NTP binding and phosphohydrolase activity associated with purified bluetongue virus non-structural protein NS2. J. Gen. Virol. 2000, 81, 1961-1965.
• 12.Kar A. K., Bhattacharaya B., Roy P.: Bluetongue virus RNA binding protein NS2 is a modulator of viral replication and assembly. BMC Mol. Biol. 2007, 8, 4.
• 13.Maan S., Maan N. S., Samuel A. R., Rao S., Attoui H., Mertens P. P.: Analysis and phylogenetic comparison of full-length VP2 genes of the 24 bluetongue virus serotypes. J. Gen. Virol. 2007, 88, 621-630.
• 14.Mattos C. C. de, Mattos C. A. de, Osburn B. I., MacLachlan N. J.: Evolution of the L2 gene of strains of bluetongue virus serotype 10 isolated in California. Virology 1994, 201, 173-177.
• 15.Mellor P. S., Wittmann E. J.: Bluetongue virus in the Mediterranean Basin 1998-2001. Vet. J. 2002, 164, 20-37.
• 16.Mertens P. P., Maan N. S., Prasad G., Samuel A. R., Shaw A. E., Portgieter A. C., Anthony S. J., Maan S.: Design of primers and use of RT-PCR assays for typing European bluetongue virus isolates: differentiation of field and vaccine strains. J. Gen. Virol. 2007, 88, 2811-2823.
• 17.Mertens P. P., Sangar D. V.: Analysis of the terminal sequences of the genome segments of four orbiviruses. Virology 1985, 140, 55-67.
• 18.Nason E. L., Rothagel R., Mukherjee S. K., Kar A. K., Forzan M., Prasad D. V., Roy P.: Interactions between the inner and outer capsids of bluetongue virus. J. Virol. 2004, 78, 8059-8067.
• 19.Owens R. J., Limn C., Roy P.: Role of an arbovirus nonstructural protein in cellular pathogenesis and virus release. J. Virol. 2004, 78, 6649-6656.
• 20.Roy P.: Bluetongue virus proteins. J. Gen. Virol. 1992, 73, 3051-3064.
• 21.Roy P.: Orbiviruses, [w:] Knipe D. M., Howley P. M., Griffin D. E., Lamb R. A., Martin M. A., Roizman B., Straus S. E. (red.): Fields Virology. PA: Lippicott Williams&Wilkins, Philadelphia 2007, 1975-1977.
• 22.Saegerman C., Berkvens D., Mellor P. S.: Bluetongue epidemiology in the European Union. Emerging Infect. Dis. 2008, 14, 539-544.
• 23.Singh K. P., Maan S., Samuel A. R., Rao S., Meyer A., Mertens P. P.: Phylogenetic analysis of bluetongue virus genome segment 6 (encoding VP5) from different serotypes. Vet. Ital. 2005, 40, 479-483.
• 24.Stauber N., Martinez-Costas J., Sutton G., Monastyrskaya K., Roy P.: Bluetongue virus VP6 protein binds ATP and exhibits an RNA-dependent ATPase function and a helicase activity that catalyse the unwinding of double stranded RNA. J. Virol. 1997, 71, 7220-7226.
• 25.Sutton G., Grimes J. M., Stuart D. I., Roy P.: Bluetongue virus VP4 is an RNA-capping assembly line. Nat. Struci. Mol. Biol. 2007, 14, 449-451.
• 26.Tan B. H., Nason E., Stauber N., Jiang W., Monastyrskaya K., Roy P.: RGD tripeptyde of bluetongue virus VP7 protein is responsible for core attachment to Culicoides wells. J. Virol. 2001, 75, 3937-3947.
• 27.Wijaszka T., Truszczyński M.: Występowanie na świecie w latach 2006-2007 ważnych epizootii, według OIE. Medycyna Wet. 2007, 63, 1270-1272.