Acetylcholinesterase from mature Hymenolepis diminuta [Cestoda]

• Autorzy: Moczon T , Swietlikowska A.
• Języki publikacji: EN

Abstrakty

EN

Acetylcholinesterase (AChE) sequentially extracted from mature specimens of Hymenolepis diminuta was shown to be a globular protein, the monomeric form of which (Ga₁) had molecular mass of 66 kDa as determined by SDS-PAGE. Amphiphilic character of the enzyme was revealed by Triton X-l14 phase partitioning. The cestode AChE preferred acetylthiocholine over propionyl- and butyrylthiocholine as substrate, split N-acetyl-ß-methylthiocholine and myristoylcholine but did not hydrolyze ß-carbonaphthoxycholine, a substrate for butyrylcholinesterases. It was sensitive to 10⁻⁵ M physostigmine and 10⁻⁵ M BW284C51 but not to 10⁻³ M iso-OMPA. No butyrylcholinesterase activity was detected in extracts from the parasite.

Słowa kluczowe

EN

Cestoda; Hymenolepis diminuta; biochemistry; parasite extract; acetylcholinesterase; electrophoresis

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2005
• Tom: 50
• Numer: 4
• Opis fizyczny: p.344-351,fig.,ref.

Twórcy

autor

Moczon T

• Polish Academy of Sciences, 51/55 Twarda Street, 00-818 Warsaw, Poland

autor

Swietlikowska A.

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