Humoralna odpowiedz na wybrane antygeny paleczek Yersinia enterocolitica i Yersinia pseudotuberculosis w przebiegu jersiniozy u ludzi. I. Wystepowanie i poziom przeciwcial dla somatycznych antygenow paleczek Yersinia oraz wydzielniczych bialek Yop wykrytych odczynem ELISA

• Autorzy: Rastawicki W.

Warianty tytułu

EN

Humoral response to selected antigens of Yersinia enterocolitica and Yersinia pseudotuberculosis in the course of yersiniosis in humans. I. Occurrence of antibodies to Yersinia lipopolisaccharydes and Yop proteins by ELISA

• Języki publikacji: PL

Abstrakty

PL

Analiza wyników badań próbek surowicy uzyskanych od osób chorych podejrzanych o jersiniozę wykazała, że humoralna odpowiedź zależna jest od objawów klinicznych, wieku badanych osób, płci i okresu choroby. Najczęściej, bez względu na dominujące objawy kliniczne, wykrywano przeciwciała dla antygenu 03 Y. enterocolitica w badanych klasach immunoglobulin A, G i M, najrzadziej zaś dla antygenu 08 pałeczek tego gatunku.

EN

The antibodies against the somatic antigens of Y. enterocolitica 03, 08, 09, 05,27,Y. pseudotuberculosis I, and released proteins Yop were detected using the ELISA in 1634 serum samples and 84 synovial fluids collected from 1290 persons suspected for yersiniosis, as well as 200 serum samples from healthy individuals (blood donors). The presence of antibody in diagnostically significant titres for somatic antigens of Yersinia were detected by ELISA in 20.5% and 50.6%, antibodies for released proteins Yop in 11.5% and 28.4% respectively of blood donors and patients suspected for yersiniosis. The antibody against the 03 antigen of Y. enterocolitica was the most frequently detected antibody while the most infrequent was the antibody for the antigen from the 08 serologic group. The results of the study showed that the humoral response picture to Yersinia antigens in the course of yersiniosis in humans is dependent on the age and sex of the patient, duration of the infection, and clinical manifestations. Most frequently the elevated antibody levels were detected among patients with erythema nodosum and patients with gastrointestinal symptoms. The frequency of occurrence of antibodies for most antigens of Yersinia, together with age increased reaching its peak, on the average, among individuals aged 21-40 years. Analysis of individual cases showed that by the end of the first week of infection, elevated levels of antibodies for somatic antigens of Yersinia are evident. On the other hand, antibodies for released proteins Yop as a matter of rule appear in the second week from the onset of clinical symptoms. Within this early phase of infection immunoglobulins of the A and M classes dominate reaching their highest level in the second to third week of the infection. In majority of the individuals studied antibodies of the IgG class reached their highest level much later in relation to those of the IgA and IgM classes. Significant differences were found in IgA antibody detection among individuals with clinical manifestations of stomachaches and arthritis. Nevertheless, among individuals with clinical symptoms of stomachaches, these immunoglobulins as a matter of principle disappear with a period of 2-3 months from the onset of clinical symptoms. In individuals with arthritis however the aforementioned immunoglobulins maintained at considerable levels even after a year. In joint-fluid samples obtained from patients with arthritis antibodies for Yersinia antigens were detected in similar levels just as obtained simultaneously serum from those individuals.

Słowa kluczowe

PL

choroby czlowieka; jersynioza; przebieg choroby; Yersinia enterocolitica; Yersinia pseudotuberculosis; antygeny; odpowiedz humoralna; przeciwciala; bialko Yop; test ELISA; wykrywanie; diagnostyka chorob

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2006
• Tom: 58
• Numer: 4
• Opis fizyczny: s.303-319,rys.,tab.,wykr.,bibliogr.

Twórcy

autor

Rastawicki W.

• Panstwowy Zaklad Higieny w Warszawie, ul.Chocimska 24, 00-791 Warszawa

Bibliografia

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