Effect of brewers' yeast [Saccharomyces cerevisiae] extract on selected parameters of humoral and cellular immunity in lambs

• Autorzy: Wojcik R
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to determine the stimulating effect of the brewers' yeast Saccharomyces cerevisiae dietary supplement on selected parameters of specific and non-specific humoral and cellular immunity in lambs. The study involved 32 lambs aged 30 ±3 d, divided into two equal groups: control and experimental. Animals in the experimental group were fed a C-J concentrate mixed with a prebiotic, the extract of dried brewers' yeast containing 10%-15% MOS and 25%-30% ß-l,3/l,6-D-glucan in the amount of 3 g/kg of the concentrate. At the beginning of the experiment (day 0) and on the 15th, 30th, and 60th d of the study, blood was sampled from the jugular vein to determine γ-globulin levels, lysozyme and ceruloplasmin activity, proliferative response of blood lymphocytes (MTT) after stimulation with LPS or ConA, metabolic activity (RBA), and potential killing activity (PKA) of phagocytes. As regards humoral immunity parameters, significantly higher γ-globulin levels and higher lysozyme and ceruloplasmin activity were noted in the blood serum of experimental lambs supplemented with the yeast extract, in comparison with control lambs not fed the supplement. No statistically significant differences in serum total protein were found between the control and experimental groups. The analysis of cellular immunity indicators revealed significantly higher levels of RBA and PKA, and higher MTT rates after stimulation with LPS or ConA in the experimental group, in comparison with the control group.

Słowa kluczowe

PL

brewery yeast; Saccharomyces cerevisiae; yeast extract; extract effect; prebiotic; protein content; humoral immunity; cellular immunity; lamb; prebiotic property; animal feeding

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 2
• Opis fizyczny: p.181-187,ref.

Twórcy

autor

Wojcik R

• University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland

Bibliografia

• 1. Akremienė D., Kondrotas A., Didžiapetrienė J., Egidijus K.: Effects of ß-glucans on the immune system. Medicina 2007, 43, 597-606.
• 2. Borchers A.T., Keen C.L., Gershwin M.E.: Mushrooms, tumors and immunity: an update. Exp Biol Med 2004, 229, 393-406.
• 3. Brown G.D., Gordon S.: Immune recognition. A new receptor for ß-glucans. Nature 2001, 413, 36-37.
• 4. Brown G.D., Gordon S.: Fungal ß-glucans and mammalian immunity. Immunity 2003, 19, 311-315.
• 5. Burkey T.E., Dritz S.S., Nietfeld J.C., Johnson B.J., Minton J.E.: Effect of dietary mannanoligosaccharide and sodium chlorate on the growth performance, acute-phase response, and bacterial shedding of weaned pigs challenged with Salmonella enterica serotype Typhimurium. J Anim Sci 2004, 82, 397-404.
• 6. Chung S., Secombes S.J.: Analysis of events occurring within teleost macrophages during the respiratory burst. Comp Biochem Physiol 1988, 89 B, 539-544.
• 7. Collins M.D., Ribson G.R.: Probiotics, prebiotics, and synbiotics: approaches for modulating the microbial ecology of the gut. Am J Clin Nutr 1999, 69, 1052S- 1057S.
• 8. Dobicki A., Preś J., Zachwieja A., Mordak R., Jakus W.: Influence of yeast preparations on chosen biochemical blood parameters and the composition of cow milk. Medycyna Wet 2007, 63, 955-959.
• 9. Grela E.R., Semeniuk V.: Consequences of the withdrawal of antibiotic growth promoters from animal feeding. Medycyna Wet 2006, 5, 502-507.
• 10. Guzdek A., Rokita H.: Curdlan sulphate modulates protein synthesis and enhances NF-κB asn C/EBP binding activity in HepG2 cells. Mediators Inflamm 1997, 6, 58- 63.
• 11. Hamuro J., Röllinghoff M., Wagner H.: Induction of cytotoxic peritoneal exudate cells by T-cell immune adjuvants of the ß(l→3)glucan-type lentinan and its analogues. Immunology 1980, 39, 551-559.
• 12. Heinrichs A.J., Jones C.M., Heinrichs B.S.: Effects of mannan oligosaccharide or antibiotics in neonatal diets on health and growth of dairy calves. J Diary Sci 2003, 86, 4064-4069.
• 13. Kataoka K., Tatsuti M., Yamazaki S., Takeshoge K.: Activation of macrophages by linear (1→3)-ß-D-glucans. J Biol Chem 2002, 277, 36825-36831.
• 14. Kokoshis P.L., Williams D.L., Cook J.A., Di Luzio N.R.: Increased resistance to Staphylococcus aureus infection and enhancement in serum lysozyme activity by glucan. Science 1978, 199, 1340-1342.
• 15. Krakowski L., Krzyżanowski J., Wrona Z., Siwicki A.K.: The effect of nonspecific immunostimulation of pregnant mares with 1,3/1,6 glucan and levamisiole on the immunoglobulin level in colostrum, selected indices of nonspecific cellular and humoral immunity in foals in neonatal and postnatal period. Vet Immunol Immunopathol 1999, 68, 1-11.
• 16. Kuprechtova D., Illek J.: Effect of mannan oligosaaccharides supplemented via milk replacer on the immune status and growth of calves. Slov Vet Zbr 2006, 43, 311-313.
• 17. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R: Protein measurements with the folin phenol reagent. J Biol Chem 1951, 193, 265-275.
• 18. Luhm J., Langenkamp U., Hensel J., Frohn C., Brand J.M., Henning H., Rink L., Koritke P., Wittkopf N., Williams D.L., Mueller A.: ß-(l→3)-D-glucan modulates DNA binding of nuclear factors κB, AT and IL-6 leading to an anti-inflammatory shift of the IL-lß/IL-1 receptor antagonist ratio. BMC Immunol 2006, 7, 5-20.
• 19. Lyons P.: A time for answer: solution for the 2001 feed industry. In: Science and Technology in the Feed Industry. Proceedings of Alltech's Seventeenth Annual Symposium, edited by Lyons T.P. and Jacques K.A., Nottingham University Press, Nottingham, 2001, pp. 1- 23.
• 20. Mao X.F., Piao X.S., Lai C.H., Li D.F., Xing J.J., Shi B.L.: Effects of β-glucan obtained from the Chinese herb Astragalus membranaceus and lipopolysaccharide challenge on performance, immunological, adrenal, and somatotropic responses of weanling pigs. J Anim Sci 2005, 83, 2775-2782.
• 21. Michalek M., Melican D., Brunke-Reese D., Langevin M., Lemerise K., Galbraith W., Patchen M., Mackin W.: Activation of rat macrophages by Betafectin® PGG- glucan requires cross-linking of membrane receptors distinct from complement receptor three (CR3). J Leukoc Biol 1998, 64, 337-344.
• 22. Milewski S., Wójcik R., Małaczewska J., Trapkowska S., Siwicki A.K.: Effect of ß-1.3/1.6-D-glucan on meat performance and non-specific humoral defense mechanisms in lambs. Medycyna Wet 2007, 63, 360-363.
• 23. Mosmann T.: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Meth 1983, 65, 55-63.
• 24. Muchmore A.V., Sathymoorty N., Decker J.M., Sherblom A.P.: Evidence that specific high-mannose oligosaccharides can directly inhibit antigen-driven T-cell responses. J Leukoc Biol 1990, 48, 457-464.
• 25. Parry R.M., Chandau R.C., Shahani R.M.: A rapid and sensitive assay of muramidase. Proc Soc Exp Biol Med 1965, 119, 384-386.
• 26. Rook G.A.W., Steele J., Umar J., Dockrel H.M.: A simple method for the solubilization of reduced NBT and its use as a colorimetric assay for activation of human macrophages by gamma interferon. J Immunol Meth 1995, 82, 161-167.
• 27. Sakurai T., Ohno N., Yadomae T.: Effects of fungal beta- glucan and interferon-γ on the secretory functions of murine alveolar macrophages. J Leukoc Biol 1996, 60, 118-124.
• 28. Savage T.F., Cotter P.F., Zakrzewska E.I.: The effect of feeding mannanoligosaccharide on immunoglobulins, plasma IgG and bile IgA of Wrolstad MW male turkeys. Poult Sci 1996, 75, 143.
• 29. Siwicki A.K., Anderson D.P.: Immunostimulation in fish: measuring the effects of stimulants by serological and immunological methods. Nordic Symposium on Fish Immunology, Lysekil, Sweden, 1993.
• 30. Siwicki A.K., Krzyżanowski J., Bartoszcze M., Mizak Z., Paluch S., Szmigielski S., Jeljaszewicz J., Pulverer G.: Adjuvant properties of killed Propionibacterium avidum KP-40 in vaccination of dogs against canine parvovirosis. Dtsch Tierärztl Wschr 1998, 105, 173-208.
• 31. Siwicki A.K., Studnicka M.: Ceruloplasmin activity in carp (Cyprinus carpio). Bamidgeh 1986, 38, 126-129.
• 32. Spring P., Privulescu M.: Mannanoligosaccharide: its logical role as a natural feed additive for piglets. In: Alltech's 14th Annual Biotechnology in the Feed Industry Symposium. Symposium Proceedings Summaries. Edited by Lyons T.P. and Jacques K.A., Nottingham University Press, Nottingham, 1998, pp. 72-73
• 33. Suzuki I., Hashimoto K., Ohno N., Yadomae T.: Immunomodulation by orally administered ß-glucan in mice. Int J Immunopharmacol 1989, 11, 761-769.
• 34. Szymańska-Czerwińska M., Bednarek D., Kowalski C.: Effect of prebiotic additives on interleukin-1 activity and alternations of peripheral blood leukocyte subpopulations in calves. Medycyna Wet 2007, 63, 1591-1594.
• 35. Wójcik R., Małaczewska J., Trapkowska S., Siwicki A.K.: Influence of ß-l,3/l,6-D-glucan on non-specific cellular defence mechanisms in lambs. Medycyna Wet 2007, 63, 84-86.
• 36. Vetvicka V., Yvin J-C.: Effects of marine ß-1,3 glucan on immune reactions. Int Immunopharm 2004, 4, 721-730.
• 37. Zhao Ch., Li M., Luo Y., Wu W.: Isolation and structural characterization of an immunostimulating polysaccharide from fuzi, Aconitum carmichaeli. Carbohydr Res 2006, 341, 485-491.