Counts and activity of microorganisms participating in nitrogen transformations in soil, four years after application of sewage sludge

• Autorzy: Joniec J , Furczak J.

Warianty tytułu

PL

Liczebnosc i aktywnosc mikroorganizmow czynnych w przemianach azotu w glebie, w czwartym roku od wzbogacenia jej osadem sciekowym

• Języki publikacji: EN

Abstrakty

EN

The objective of the study was to determine the direction, intensity and duration of changes in abundance and activity of certain microbial groups active in nitrogen transformations in soil subjected to a 4-year fertilization treatment with municipal and industrial sewage sludge. The study was conducted on podzolic soil, whose accumulation horizon had been fertilized in 1998 with fermented sewage sludge at doses of 30 Mg⋅ha-1(1%), 75 Mg⋅ha-1(2.5%), 150 Mg⋅ha-1(5%), 300 Mg⋅ha-1(10%) and 600 Mg⋅ha-1(20%) and planted with willow (Salix viminalis L.). Four years after the application of sludge, microbiological and biochemical analyses were made in two soil horizons (0-20 cm and 20-40 cm). It was found that in the soil from the 0-20 cm depth significant stimulation of the growth of proteolytic fungi and bacteria continued, but only under the effect of the highest dose of sludge. Moreover, there was a notable stimulation of protease activity and nitrification process alongside a slight inhibition of ammonification. In the soil from the 20-40 cm layer stimulation of the growth of protein-decomposing fungi was observed as well as that of proteolytic and nitrification activity of soil, while ammonification was inhibited. However, the effect of sludge was generally less pronounced in the deeper soil layer than in the surface soil horizon.

PL

Celem pracy było określenie kierunku, natężenia i czasu utrzymywania się zmian w liczebności i aktywności niektórych grup mikroorganizmów czynnych w przemianach azotu, w glebie poddanej 4-letniemu oddziaływaniu osadu ścieków komunalno-przemysłowych. Badaniami objęto glebę bielicową, której poziom akumulacyjny nawieziono w 1998 r. przefermentowanym osadem ściekowym w ilości: 30 Mg·ha-1(1%), 75 Mg·ha-1(2,5%), 150 Mg·ha-1 (5%), 300 Mg·ha-1(10%) i 600 Mg·ha-1(20%). Następnie glebę obsadzono wierzbą (Salix viminalis L.) i w 4-tym roku od wprowadzenia osadu wykonano w dwu jej warstwach (0-20 cm i 20-40 cm) analizy mikrobiologiczne i biochemiczne. Stwierdzono, że w glebie z głębokości 0-20 cm utrzymywało się nadal istotne pobudzenie rozwoju bakterii i grzybów proteolitycznych, ale tylko pod wpływem najwyższej dawki osadu. Ponadto występowała wyraźna stymulacja aktywności proteazy i procesu nitryfikacji oraz niewielkie hamowanie amonifikacji. W glebie z warstwy 20-40 cm odnotowano również stymulację rozwoju grzybów rozkładających białko, aktywności proteolitycznej i nitryfikacyjnej gleby oraz hamowanie amonifikacji. Jednak oddziaływanie osadu było w tych warunkach na ogół słabsze niż w wierzchniej warstwie gleby.

Słowa kluczowe

EN

municipal-industrial sewage sludge; podzolic soil; nitrogen; microbiological transformation; nitrogen transformation

• Wydawca: -
• Czasopismo: Journal of Elementology
• Rocznik: 2008
• Tom: 13
• Numer: 4
• Opis fizyczny: p.545-557,ref.

Twórcy

autor

Joniec J

• University of Life Sciences in Lublin, Leszczynskiego 7, 20-029 Lublin, Poland

autor

Furczak J.

Bibliografia

• Baran S., Bielińska E.J., Wiśniewski J. 1999. Effect of sewage sludge and vermicompost of the sludge on enzymatic activity of a sandy soil. Ann. UMCS, Sect. E, 54: 145-151.
• Baran S., Bielińska E.J., Wójcikowska-Kapusta A. 2000. Effect of willow culture on dehydrogenase and phosphatase activity and lead content in podzolic soil amended with sewage sludge. Fol. Univer. Agric. Stetin., 211, Agric., 84: 19-24.
• Baran S., Domżał H., Sławińska-Jurkiewicz A., Kwiecień J., Pranagal J. 1996a. Effect of sewage sludge on water-air relations in a sandy soil. Zesz. Probl. Post. Nauk Rol., 437: 53-60.
• Baran S., Sławińska-Jurkiewicz A., Pranagal J. 1996b. Morphological analysis of structure of sandy soil amended with sewage sludge. Zesz. Probl. Post. Nauk Rol., 437: 61-68.
• Bielińska E.J., Żukowska G. 2002. Protease activity in light soil amended with sewage sludge. Acta Agrophysica., 70: 41-47.
• Chazijew F.Ch.1982. Sistemno-ekologiceskij analiz fiermentativnoj aktiwnosti pocw. Nauka, Moskva.
• Cieśla W., Koper J. 1990. Effect of multiple mineral-organic fertilisation on the level of organic and available phosphorus and enzymatic activity of soil. Rocz. Glebozn., 41: 73-83.
• Czekała J. 2002. Selected properties of sewage sludge from sewage treatment plants in the Wielkopolska Region. Part I. Reaction, dry mass, organic matter carbon, and macrocomponents. Acta Agrophysica, 70: 75-82.
• Dick R.P. 1994. Soil enzyme activities as indicators of soil quality. In: Defining soil quality for a sustainable environment. Doran J.V., Coleman D.C., Bezdicek D.F., Stewart B.A. Soil Sci. Soc. Am., Am. Soc. Agric., Madison, 107-124.
• Furczak J., Joniec J. 2007a. Numbers of microorganisms and activity of biochemical processes related with carbon transformations in podzolic soil under willow culture, in fourth year of soil treatment with sewage sludge. Pol. J. Environ. Stud., 16: 587-592.
• Furczak J., Joniec J. 2007b. Preliminary study of sludge effect on soil microbial activity of podzolic soil under willow culture. Int. Agrophysics, 21: 39-47.
• Furczak J., Joniec J. 2007c. Changes in biochemical activity of podzolic soil under willow culture in the second year of treatment with municipal-industrial sewage sludge. Int. Agrophysics, 21: 145-152.
• Garcia-Gil G.L., Plaza C., Senesi N., Brunetti G., Polo A. 2004. Effect of sewage sludge amendment on humic acids and microbiological properties of a semiarid Mediterranean soil. Biol. Fertil. Soils, 39: 320-328.
• Hattori H., Mukai S. 1986. Decomposition of sewage sludge in soil as affected by their organic matter composition. Soil Sci. Plant Nutr., 32: 421-432.
• Herrero O., Canet R., Albiach R., Pomares F. 1998. Enzymatical activities and content of mineral nitrogen in soil after the application of two rates of different organic products. Agrochimica, 62: 296-303.
• Joniec J., Furczak J. 2007a. Numbers of selected microbial groups in a podzolic soil under willow culture, amended with sewage sludge, in the second year of its effect. Ann. UMCS, Sect. E, 62: 93-104.
• Joniec J., Furczak J. 2007b. Biochemical characterization of podzolic soil under willow culture after three year from its amendment with sewage sludge. Pol. J. Soil Sci., 40: 207-215.
• Joniec J., Furczak J. 2007c. Numbers of selected microbial groups in a podzolic soil under willow culture (Salix viminalis L.), amended with sewage sludge, in the third year of its effect. Ann. UMCS, Sect. E, 62: 105-116.
• Kobus J. 1995. Biological processes and soil fertility. Zesz. Probl. Post. Nauk Rol., 421a: 209-219.
• Kobus J., Czaban J., Gajda A. 1990. Effect of sewage sludge on the activity of degraded soils on carbon, nitrogen, phosphorus and zinc transformations in such soils. Pam. Puł. — Pr. IUNG, 96: 121-137.
• Ladd J.N., Butler J.A.H. 1972. Short-term assays of soil proteolytic enzyme actiyities using proteins and dipeptide derivatives as substrates. Soil Biol. Biochem., 4: 19-30.
• Martin J. 1950. Use of acid rose bengal and streptomycin in the plate method for estimating soil fungi. Soil Sci., 19: 215-233.
• Nowosielski O. 1974. Methods for determination of fertilization requirements. PWRiL, Warszawa.
• Pascual I., Antolin C.M., Garcia C., Polo A., Sanchez-Diaz M. 2007. Effect of water deficit on microbial characteristics in soil amended with sewage sludge or inorganic fertilizer under laboratory conditions. Biores. Techn., 98: 29-37.
• Paul E.A. Clark F.E. 2000. Microbiology and biochemistry of soils. UMCS, Lublin.
• Rodina A. 1968. Microbiological methods for the study of waters. PWRiL, Warszawa.
• Sastre J., Vincente M.A., Lobo H.C. 1996. Influence of the application of sewage sludge on soil microbial activity. Biores. Techn., 57: 19-23.
• Siuta J.1998. Conditions and methods of agricultural utilisation of sewage sludge. In: The bases and practice of recovery and utilization sludges. Proc. Inter. Sem., 4.1-4.19, LEM s.c., Kraków.
• Szczukowski S., Tworkowski J., Stolarski M. 2001. Biomass of fruticose willows obtained from field plantations as a source of ecological fuel and raw materials. Zesz. Probl. Post. Nauk Rol., 478: 343-350.
• Wick B., Kuhne R.F., Vielhauer K., Vlek P.L.G. 2002. Temporal variability of selected soil microbiological and biochemical indicators under different soil quality conditions in south-western Nigeria. Biol. Fertil, Soils, 35: 155-167.
• Żukowska G., Flis-Bujak M., Baran S. 2002. Effect of fertilisation with sewage sludge on organic matter of light soil under willow culture. Acta Agrophysica, 73: 357-367.