A note on the growth hormone [GH1-AluI] polymorphism in Podolian cattle in Southern Italy

• Autorzy: Dario C , Carnicella D. , Bufano G.
• Języki publikacji: EN

Abstrakty

EN

Individual blood samples were collected from 132 unrelated Podolian cattle belonging to 13 farms located in the inland territories of Southern Italy. Genomic DNA samples were genotyped for the GH1-AluI polymorphism by Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP). The mutation analysed causes the amino acid substitution leucine→valine at position 127 of the GH polypeptide. Allele frequencies for GH1 A and GH1 B were 0.85 and 0.15, respectively. Hardy-Weinberg equilibrium was found in the analysed animals. Due to the rather low frequency of GH1 B in the breed, a larger sample including a higher number of animals carrying GH1 B is necessary to assess possible association between GH1-AluI genotypes and beef traits in the Podolian cattle.

PL

Krew pobrano od niespokrewnionego ze sobą bydła podolskiego (n=132) utrzymywanego w 13 fermach na terenie południowych Włoch, w rejonach Basilicata i Apulia. Bydło zwane tu podolskim reprezentowało do niedawna typ użytkowy roboczy, a obecnie prowadzone są prace nad przekształceniem go w typ dwukierunkowy, z przewagą mięsności. Próbki genomowego DNA genotypowano pod kątem widzenia polimorfizmu genu GH1-AluI, posługując się metodą PCR-RFLP. Analizowana mutacja polega na zastąpieniu leucyny waliną w pozycji 127 polipeptydu GH. Frekwencje alleli GH1 A i GH1 B wyniosły odpowiednio 0,85 i 0,15. Badany materiał pozostawał w równowadze genetycznej zgodnie z prawem Hardy-Weinberga. Autorzy wnioskują, że z racji niskiej częstości występowania GH1 B, do wykrycia ewentualnego związku między polimorfizmem genu GH1-AluI a cechami mięsności bydła podolskiego potrzebne jest zbadanie większej populacji zwierząt, która zawierałaby więcej nosicieli GH1 B.

Słowa kluczowe

EN

beef trait; GH1-AluI genotype; gene polymorphism; Italy; cattle; Southern Italy; growth hormone; Podolian breed; animal health

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2005
• Tom: 23
• Numer: 1
• Opis fizyczny: p.43-49,fig.,ref.

Twórcy

autor

Dario C

• University of Study of Bari, Strada Provinciale per Casamassima km 3, 70010 Valenzano [Ba], Italy

autor

Carnicella D.

autor

Bufano G.

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