The effect of mono- and divalent cations on Tetrahymena thermophila telomeric repeat fragment. A photon correlation spectroscopy study

• Autorzy: Wlodarczyk A. , Patkowski A. , Grzybowski P. , Dobek A.
• Języki publikacji: EN

Abstrakty

EN

The structure of the Tetrahymena thermophila telomeric sequence d(TGGGGT)4 was studied by photon correlation spectroscopy (PCS) in aqueous solution in the presence of NaCl, KCl and SrCl2. The sample studied was polydisperse in all conditions studied. Translational diffusion coefficients DT describing the diffusion modes observed were determined. On the basis of a comparison between the experimental DT values with those calculated assuming the bead model, two forms were identified as telomeric quadruplex structures: monomer and tetramer. In the presence of SrCl2 formation of aggregates was observed, with a size that reached several micrometres. The relative weighted concentrations of the structures observed for different concentrations of a salt and DNA were determined. The results obtained in the presence of monovalent ions were qualitatively similar and could be presented in a coherent plot in which the concentration of salt was expressed by the number of ions per DNA molecule. A large number of ions per DNA molecule favoured tetramer formation while a small number favoured the monomer form. A structural phase transition from the monomer to the tetramer induced by a change in the number of ions per DNA molecule was observed. The main difference between the results for Na+ and K+ was a greater effectiveness of the K+ ions in formation of tetramers. The effect of Sr2+ ions on the structures formed was different than that of the monovalent ions. The results obtained in the presence of Sr2+ could not be described as a function of the number of ions per DNA molecule.

Słowa kluczowe

EN

chromosome; structure transition; telomeric DNA; photon correlation spectroscopy; telomeric sequence; Tetrahymena thermophila

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 4
• Opis fizyczny: p.971-981,fig.,ref.

Twórcy

autor

Wlodarczyk A.

• Royal Institution of Great Britain, 21 Albemarle Street, London W1S 4BS, U.K.

autor

Patkowski A.

autor

Grzybowski P.

autor

Dobek A.

Bibliografia

• Banachowicz E, Gapinski J, Patkowski A. (2000) Solution structure of biopolymers: a new method of constructing a bead model. Biophys J.; 78: 70-8.
• Biessmann H, Mason JM. (1994) Telomeric repeat sequences. Chromosoma.; 103: 154-61.
• Blackburn EH. (1999) The telomere and telomerase: how do they interact? Mt Sinai J Med.; 66: 292-300.
• Blackburn EH. (1991) Structure and function of telomeres. Nature.; 350: 569-73.
• Chen F-M. (1992) Sr2+ faciliates intermolecular G-quadruplex formation of a telomeric sequence. Biochemistry.; 31: 3769-76.
• Greider CW. (1999) Telomeres do D-loop-T-loop. Cell.; 97: 419-22.
• Greider CW, Blackburn EH. (1985) Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell.; 43: 405-13.
• Griffith JD, Comeau L, Rosenfield S, Stansel RM, Bianchi A, Moss H, de Lange T. (1999) Mammalian telomeres end in a large duplex loop. Cell.; 97: 503-14.
• Hardin CC, Henderson E, Watson T, Prosser JK. (1991) Monovalent cation-induced structural transitions in telomeric DNAs: G-DNA folding intermediates. Biochemistry.; 30: 4460-72.
• Henderson E, Hardin CC, Walk SK, Tinoco I Jr, Blackburn EH. (1987) Telomeric DNA oligonucleotides form novel intramolecular structures containing guanine-guanine base pairs. Cell.; 51: 899-908.
• Keniry MA. (2001) Quadruplex structures in nucleic acids. Biopolymers.; 56: 123-46.
• Lee JS. (1990) The stability of polypurine tetraplexes in the presence of mono- and divalent cations. Nucleic Acids Res.; 18: 6057-60.
• Linger J, Cooper JP, Cech TR. (1995) Telomerase and DNA end replication: no longer a lagging strand problem? Science.; 269: 1533-54.
• Liu K, Schoonmaker MM, Levine BL, June CH, Hodes RJ, Weng N. (1999) Constitutive and regulated expression of telomerase reverse transcriptase (hTERT) in human lymphocytes. Proc Natl Acad Sci USA.; 96: 5147-52.
• Makarov VL, Hirose Y, Langmore JP. (1997) Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening. Cell.; 88: 647-66.
• Marsh TC, Vesenka J, Henderson E. (1995) A new DNA nanostructure, the G-wire, imaged by scanning probe microscopy. Nucleic Acids Res.; 23: 696-700.
• McCormick-Graham M, Haynes WJ, Romero DP. (1997) Variable telomeric repeat synthesis in Paramecium tetraurelia is consistent with misincorporation by telomerase. EMBO J.; 16: 3233-42.
• Miura T, Thomas GJ Jr. (1994) Structural polymorphism of telomere DNA: interquadruplex and duplex- quadruplex conversions probed by Raman spectroscopy. Biochemistry.-, 33: 7848-56.
• Miura T, Benevides JM, Thomas GJ Jr. (1995) A phase diagram for sodium and potassium ion control of polymorphism in telomeric DNA. J Mol Biol.; 248: 233-8.
• Neidle S, Read MA. (2001) G-Quadruplexes as therapeutic targets. Biopolymers.; 56: 195-208.
• Rotne J, Prager S. (1969) Variational treatment of hydrodynamic interaction in polymers. J Chem Phys.; 50: 4831-7.
• Sen D, Gilbert W. (1990) A sodium-potassium switch in the formation of four-stranded G4-DNA. Nature.; 344: 410-4.
• Sen D, Gilbert W. (1992) Guanine quartet structures. Methods Enzymol.; 211: 191-9.
• Wang Y, Patel DJ. (1994) Solution structure of the Tetrahymena telomeric repeat d(T2G4)4 G-tetraplex. Structure.; 2: 1141-56.
• Williamson JR. (1994) G-quartet structures in telomeric DNA. Annu RevBiophys Biomol Struct.; 23: 703-30.
• Williamson JR, Raghuraman MK, Cech TR. (1989) Monovalent cation-induced structure of telomeric DNA: the G-quartet model. Cell.; 59: 871-80.
• Wlodarczyk A, Gapinski J, Patkowski A, Dobek A. (1999) Structural polymorphism of telomeres studied by photon correlation spectroscopy. Acta Biochim Polon.; 46: 609-13.