Different beta-adrenergic-specific molecular probes reveal the same DNA species in Paramecium hybridization analysis

• Autorzy: Surmacz L. , Krawczyk K. , Wyroba E.
• Języki publikacji: EN

Abstrakty

EN

In Southern blot analysis of Paramecium genomic DNA using β-adrenergic specific oligonucleotides labelled with Digoxigenin-ddUTP, the DNA species of the same molecular size were detected by different molecular probes. The 6.5 kb DNA species hybridized with the probes directed to the adjacent β2-receptor transmembrane domains: to TM3 - including Asp 113 involved in β-agonists and antagonists binding, and to TM4 - the oligonucleotide being one of the "gene-specific universal mammalian sequence-tagged site”. Since a profound physiological effect of beta-agonists and antagonists has been previously observed in Paramecium, such a hybridization pattern may suggest an existence of Paramecium DNA sequences homologous to those of metazoan eukaryotes encoding for a membrane beta-adrenergic receptor.

Słowa kluczowe

EN

Paramecium; hybridization; beta-adrenergic receptor; molecular probe; digoxigenin

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 1997
• Tom: 02
• Numer: 4
• Opis fizyczny: p.379-387,fig.

Twórcy

autor

Surmacz L.

• Nencki Institute of Experimental Biology, Pasteura 3, 02-093 Warsaw, Poland

autor

Krawczyk K.

autor

Wyroba E.

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