Isolation, identification and pathogenicity assessment of a new isolate of entomopathogenic fungus, Beauveria bassiana in Iran

• Autorzy: Safavi S A

Warianty tytułu

PL

Izolacja, identyfikacja i okreslenie nowego izolatu entomopatogenicznego grzyba Beauveria bassiana w Iranie

• Języki publikacji: EN

Abstrakty

EN

A new isolate (BEH) of entomopathogenic fungus, Beauveria bassiana was isolated from soil using DOC2 selective medium. This isolate was characterized by conidiophores consisting of whorls and dense clusters of short conidiophorous cells with one-celled spherical conidia. Conidial length and width were 2.27±0.22 μm and 1.85±0.32 μm, respectively with length/width ratio of 1.23. Colonies on SDYA medium were normally white to pale yellow and sometimes red pigmented in reverse. Because of importance of this pathogen in biocontrol programs around the world and difficulties with morphological identification, a molecular technique was developed to assist complementary identification of the fungus. Pr1, a pathogenicity-related alkaline cuticle-degrading serine protease, with defined sequence in B. bassiana was amplified using PCR technique. The presence of this gene in isolated fungus (BEH) with 744 bp sequence length, as visualized on agarose gel affirmed the data from morphological studies that the new isolate (BEH) pertained to entomopathogenic fungus, B. bassiana. Pathogenicity of new isolate against Tenebrio molitor and its recovering was the other confirmation that the isolated fungus belonged to B. bassiana, using further light microscope studies.

PL

Nowy izolat (BEH) wyosobniono z ziemi wykorzystując selektywną pożywkę DOC2. Izolat ten charakteryzował się trzonkami konidialnymi składającymi się z gęstych skupień krótkich komórek konidioforów z jednokomórkowymi, sferycznymi konidiami. Długość i szerokość konidiów wynosiła odpowiednio - 2,27±0,22 μm i 1,85±0,32 μm, przy stosunku długości do szerokości 1,23. Kolonie na pożywce SDYA pierwotnie były barwy od białej do jasno-żółtej czasem od spodu były zabarwione na czerwono. Z powodu ważności tego patogena w programach biologicznego zwalczania i trudności z morfologiczną identyfikacją, opracowano technikę molekularną w celu uzupełnienia procedury określenia grzyba. Wykorzystując technikę PCR amplifikowano związaną z patogenicznością alkaliczną protezę seryny Pr1 degradującą kutikulę (skórkę), z określoną sekwencją w B. bassiana. Obecność tego genu w wyosobnionym grzybie (BEH) z sekwencją o długości 744 bp, potwierdziła dane uzyskane w toku badań morfologicznych - nowy izobat należy do entomopatogenicznego gatunku B. bassiana. Patogeniczność nowego izolatu dla Tenebrio monitor i jego reizolacja jest także potwierdzeniem przynależności do B. bassiana, co było zgodne z badaniem mikroskopem świetlnym.

Słowa kluczowe

EN

isolation; identification; pathogenicity; assessment; new isolate; isolate; entomopathogenic fungi; Beauveria bassiana; Iran; virulence; morphology

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2010
• Tom: 50
• Numer: 2
• Opis fizyczny: p.158-163,fig.,ref.

Twórcy

autor

Safavi S A

• Urmia University, Urmia, Iran

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