Nanospray mass spectrometry for identification of peptides. Application of a novel interface

• Autorzy: Smoluch M. T. , Sallberg M. , Silberring J.
• Języki publikacji: EN

Abstrakty

EN

Electrospray ionization mass spectrometry is a powerful tool for identification of biomolecules such as peptides, proteins, oligosaccharides and neurotransmitters. Recent development of the nanospray techniques, applied at ultralow flow-rates, allowed a sensitive analysis of compounds at femto/attomolar level. Here, we present application of a novel nanospray device for the analysis and fragmentation of peptides with high sensitivity on a sector instrument. The lowest applied flow-rate of the mobile phase was maintained at 50 nl/min with a sample load of 90 fmol. Nanospray also provided a complete analysis of 500 nl of the sample for over 10 min, including sequencing of as little as 40 pmol of a substance. Such analysis provides full structural information necessary to identify the molecules.

Słowa kluczowe

EN

collision-induced dissociation; peptide; mass spectrometry; electrospray ionization mass spectrometry; sequencing

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 2
• Opis fizyczny: p.475-481,fig.

Twórcy

autor

Smoluch M. T.

• Jagiellonian University, R.Ingardena 3, 30-060 Krakow, Poland

autor

Sallberg M.

autor

Silberring J.

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