Functional and physical interactions of Krr1p, a Saccharomyces cerevisiae nucleolar protein

• Autorzy: Gromadka R. , Karkusiewicz I. , Rempola B. , Rytka J.
• Języki publikacji: EN

Abstrakty

EN

The Krr1 protein of Saccharomyces cerevisiae is involved in processing of pre-rRNA and assembly of pre-ribosomal 40S subunits. To further investigate the function of Krr1p we constructed a conditional cold sensitive mutant krrl-21, and isolated seven genes from Schizosaccharomyces pombe whose products suppressed the cold sensitive phenotype of krrl-21 cells. Among the multicopy suppressors we found genes coding for translation elongation factor EF-1a, a putative ribose methyltransferase and five genes encoding ribosomal proteins. Using the tandem af­finity purification (TAP) method we identified thirteen S. cerevisiae ribosomal pro­teins interacting with Krr1p. Taken together, these results indicate that Krr1p inter­acts functionally as well as physically with ribosomal proteins. Northern blot analy­sis revealed that changes in the level of krrl-21 mRNA were accompanied by similar changes in the level of mRNAs of genes encoding ribosomal proteins. Thus, Krr1p and the genes encoding ribosomal proteins it interacts with seem to be coordinately regulated at the level of transcription.

Słowa kluczowe

EN

transcription; functional interaction; physical interaction; ribosomal protein; nucleolar protein; Saccharomyces pombe; suppressor; Saccharomyces cerevisiae

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 1
• Opis fizyczny: p.173-187,fig.,ref.

Twórcy

autor

Gromadka R.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Karkusiewicz I.

autor

Rempola B.

autor

Rytka J.

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